摘要:
Provided herein are a primer set capable of amplifying target sequence(s) of nine respiratory disease-associated bacterial species, a probe set specifically hybridizing with the target sequence(s), a microarray comprising the probe set, and a method of detecting one or more of the nine respiratory disease-associated bacterial species using the probe set.
摘要:
A method of increasing the specificity of nucleic acid hybridization, comprising hybridizing nucleic acid in a solution containing a zwitterionic compound selected from the group consisting of 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), 3-(cyclohexylamino)-2-hydroxy-1-propane sulfonate (CAPSO), and 2-(cyclohexylamino)ethane sulfonate (CHES) is provided. The method allows a reduction in the yield of non-specific amplification products in multiplex PCRs while maintaining the yield of target amplification products.
摘要:
Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R1, R2, R3, R10, n and l are as defined in the specification.
摘要:
Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R1, R2, R3, R10, n and I are as defined in the specification.