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13 条记录 (耗时 0.02 秒)

    METHOD OF INCREASING SPECIFICITY OF NUCLEIC ACID HYBRIDIZATION USING ZWITTERIONIC COMPOUND
    1.
    发明申请
    METHOD OF INCREASING SPECIFICITY OF NUCLEIC ACID HYBRIDIZATION USING ZWITTERIONIC COMPOUND 审中-公开
    使用ZWITTERICIC化合物增加核酸杂交特异性的方法

    公开(公告)号：US20080199857A1

    公开(公告)日：2008-08-21

    申请号：US11612862

    申请日：2006-12-19

    申请人： Jung-nam LEE ,  Sang-hyun PEAK

    发明人： Jung-nam LEE ,  Sang-hyun PEAK

    IPC分类号： C12Q1/68 ,  C07H1/00 ,  C12P19/34

    CPC分类号： C12Q1/6832 ,  C12Q1/6848 ,  C12Q2527/125

    摘要： A method of increasing the specificity of nucleic acid hybridization, comprising hybridizing nucleic acid in a solution containing a zwitterionic compound selected from the group consisting of 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), 3-(cyclohexylamino)-2-hydroxy-1-propane sulfonate (CAPSO), and 2-(cyclohexylamino)ethane sulfonate (CHES) is provided. The method allows a reduction in the yield of non-specific amplification products in multiplex PCRs while maintaining the yield of target amplification products.

    摘要翻译： 一种提高核酸杂交特异性的方法，包括将核酸混合于含有选自3-（环己基氨基）-1-丙磺酸（CAPS），3 - [（3-胆酰胺丙基） 二甲基铵] -1-丙磺酸盐（CHAPS），3-（环己基氨基）-2-羟基-1-丙磺酸盐（CAPSO）和2-（环己基氨基）乙烷磺酸盐（CHES）。 该方法允许在多重PCR中降低非特异性扩增产物的产量，同时保持靶扩增产物的产量。

    APPARATUS AND METHOD FOR REDUCING NON-SPECIFIC AMPLIFICATION IN MULTIPLEX PCR
    3.
    发明申请
    APPARATUS AND METHOD FOR REDUCING NON-SPECIFIC AMPLIFICATION IN MULTIPLEX PCR 审中-公开
    用于减少多重PCR中非特异性扩增的装置和方法

    公开(公告)号：US20080124769A1

    公开(公告)日：2008-05-29

    申请号：US11936321

    申请日：2007-11-07

    申请人： Sang-hyun PAEK ,  Jung-nam LEE

    发明人： Sang-hyun PAEK ,  Jung-nam LEE

    IPC分类号： C12P19/34 ,  C12M1/00

    CPC分类号： B01L3/50853 ,  B01L3/50851 ,  B01L2200/141 ,  B01L2300/042 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851

    摘要： Provided are a well plate for multiplex PCR including: a plurality of wells for multiplex PCR each well comprising at least two partitioned chambers formed by at least one barrier that extends in an upward direction from the bottom of the wells and has a lower height than the height of the wells, wherein the wells optionally comprise plugs that seal the partitioned chambers to be separated from each other, a multiplex PCR apparatus including the well plate, and a method of performing multiplex PCR using the multiplex PCR apparatus.

    摘要翻译： 提供了一种用于多重PCR的孔板，其包括：用于多重PCR的多个孔，每个孔包括由至少一个屏障形成的至少两个分隔室，所述至少一个屏障从孔的底部沿向上的方向延伸，并且具有比 孔的高度，其中所述孔任选地包括密封彼此分离的分隔室的塞子，包括所述孔板的多重PCR装置以及使用所述多重PCR装置进行多重PCR的方法。

    LYOPHILIZING TRAY EMPLOYING REAGENT CARTRIDGE, LYOPHILIZING SYSTEM EMPLOYING THE SAME, AND METHOD OF PREPARING LYOPHILIZED REAGENT
    4.
    发明申请
    LYOPHILIZING TRAY EMPLOYING REAGENT CARTRIDGE, LYOPHILIZING SYSTEM EMPLOYING THE SAME, AND METHOD OF PREPARING LYOPHILIZED REAGENT 有权
    使用试纸盒的LYOPHILISING托盘，使用该试剂盒的LYOPHILISING系统和制备LYOPHILED试剂的方法

    公开(公告)号：US20100101106A1

    公开(公告)日：2010-04-29

    申请号：US12574206

    申请日：2009-10-06

    申请人： Do-gyoon KIM ,  Han-sang KIM ,  Jung-nam LEE

    发明人： Do-gyoon KIM ,  Han-sang KIM ,  Jung-nam LEE

    IPC分类号： F26B5/06 ,  F26B25/08

    CPC分类号： F26B5/06 ,  B01L3/52 ,  F26B25/001 ,  G01N35/1002

    摘要： A method of manufacturing a lyophilized reagent, including mounting a plurality of reagent cartridges in which a liquid reagent is loaded in a tray including one or more tunnel-shaped first rails having opened end portions in a lengthwise direction and an opening formed in the top of the one or more first rails; closing the opened end portions, placing the tray in a lyophilizer and lyophilizing the liquid reagent according to a lyophilizing program.

    摘要翻译： 一种冻干试剂的制造方法，其特征在于，将多个试剂盒安装在其中，所述试剂盒中装有液体试剂，所述试剂盒包括在长度方向上具有开口端部的一个或多个隧道状的第一导轨的托盘， 一个或多个第一轨道; 关闭打开的端部，将托盘放置在冻干机中，并根据冻干程序冻干液体试剂。

    METHOD OF DESIGNING PROBES FOR DETECTING TARGET SEQUENCE AND METHOD OF DETECTING TARGET SEQUENCE USING THE PROBES
    8.
    发明申请
    METHOD OF DESIGNING PROBES FOR DETECTING TARGET SEQUENCE AND METHOD OF DETECTING TARGET SEQUENCE USING THE PROBES 有权
    设计用于检测目标序列的探针的方法和使用探测器检测目标序列的方法

    公开(公告)号：US20080139398A1

    公开(公告)日：2008-06-12

    申请号：US11739354

    申请日：2007-04-24

    申请人： Tae-jin AHN ,  Ji-young OH ,  Jung-nam LEE ,  Jong-suk CHUNG

    发明人： Tae-jin AHN ,  Ji-young OH ,  Jung-nam LEE ,  Jong-suk CHUNG

    IPC分类号： C40B30/04 ,  G01N33/50 ,  C07H21/04

    CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/689 ,  Y10T436/145555 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2525/185

    摘要： A method of designing probes for detecting a target sequence and a method of detecting target sequence using the probes are provided. The method of designing probes for detecting target sequence includes: selecting an anchoring location in the target sequence, wherein the anchoring location is a location in the target sequence at which a first non-target sequence in a plurality of non-target sequences is different from the target sequence, wherein the first non-target sequence has a sequence similarity to the target sequence that is identical to or higher than the sequence similarity of other non-target sequences in the plurality of non-target sequences; selecting a first probe designing region, wherein the first probe designing region is a fixed region in the target sequence comprising the anchoring location; selecting a probe selection location, wherein the probe selection location is a location in the target sequence at which a second non-target sequence in the plurality of non-target sequences is different from the target sequence; selecting a matched probe, wherein the matched probe comprises a sequence complementary to the target sequence, wherein the probe selecting location is at the center of the sequence of the matched probe; and selecting a mismatched probe, wherein the mismatched probe is longer than the matched probe, wherein the mismatched probe comprises a sequence complementary to the target sequence at all locations except at a mismatched location, wherein the probe selection location is at a first location in the sequence of the mismatched probe and the mismatched location is at a second location in the sequence of the mismatched probe.

    摘要翻译： 提供了一种设计用于检测靶序列的探针的方法和使用探针检测靶序列的方法。 设计用于检测目标序列的探针的方法包括：在目标序列中选择锚定位置，其中锚定位置是目标序列中多个非靶序列中的第一非靶序列不同于 所述靶序列，其中所述第一非靶序列与所述靶序列具有与所述多个非靶序列中的其他非靶序列的序列相似性相同或更高的序列相似性; 选择第一探针设计区域，其中所述第一探针设计区域是包括所述锚定位置的所述目标序列中的固定区域; 选择探针选择位置，其中所述探针选择位置是所述多个非靶序列中的第二非靶序列与所述靶序列不同的所述靶序列中的位置; 选择匹配的探针，其中所述匹配的探针包含与所述靶序列互补的序列，其中所述探针选择位置位于所述匹配探针的序列的中心; 以及选择不匹配的探针，其中所述不匹配的探针长于所述匹配的探针，其中所述不匹配的探针包括除所述不匹配位置之外的所有位置处与所述靶序列互补的序列，其中所述探针选择位置位于所述匹配探针的第一位置 不匹配探针和不匹配位置的序列位于错配探针序列中的第二个位置。

    METHOD AND COMPOSITIONS FOR DISCRIMINATING GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA
    9.
    发明申请
    METHOD AND COMPOSITIONS FOR DISCRIMINATING GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIA 审中-公开
    用于分离GRAM-Negative和GRAM-POSITIVE BACTERIA的方法和组合物

    公开(公告)号：US20080118923A1

    公开(公告)日：2008-05-22

    申请号：US11739437

    申请日：2007-04-24

    申请人： Kyung-hee PARK ,  Jung-nam LEE ,  Sook-young KIM

    发明人： Kyung-hee PARK ,  Jung-nam LEE ,  Sook-young KIM

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/689 ,  C12Q2600/16

    摘要： Provided are an oligonucleotide primer set for amplifying at least one target sequence of the 16S rRNA gene in gram-negative bacteria and at least one target sequence of the 16S rRNA gene in gram-positive bacteria, a probe set specifically hybridizing with at least one target sequence of the 16S rRNA gene in gram-negative bacteria and at least one target sequence of the 16S rRNA gene in gram-positive bacteria, a microarray comprising the probe set, a method of discriminating gram-negative bacteria and gram-positive bacteria, and a kit for discriminating gram-negative bacteria and gram-positive bacteria, including the primer set.

    摘要翻译： 提供了用于扩增革兰氏阴性细菌中16S rRNA基因的至少一个靶序列和革兰氏阳性细菌中16S rRNA基因的至少一个靶序列的寡核苷酸引物组，与至少一个靶标特异性杂交的探针组 革兰氏阴性细菌中16S rRNA基因的序列和革兰氏阳性细菌中16S rRNA基因的至少一个靶序列，包含探针组的微阵列，区分革兰氏阴性细菌和革兰氏阳性细菌的方法，以及 用于区分革兰氏阴性细菌和革兰氏阳性菌的试剂盒，包括引物组。