公开(公告)号：US20130237432A1

公开(公告)日：2013-09-12

申请号：US13807660

申请日：2011-06-30

申请人： Jian Li ,  Shiping Chen ,  Xiandong Zhang ,  Ying Liu ,  Calfen Zhang ,  Tao Liu ,  Meiru Zhao

发明人： Jian Li ,  Shiping Chen ,  Xiandong Zhang ,  Ying Liu ,  Calfen Zhang ,  Tao Liu ,  Meiru Zhao

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  C12Q2525/191

摘要： The invention provides a PCR sequencing method, wherein the combination of primer indexes, DNA incomplete shearing strategy and the second generation sequencing technique (Paired-End sequencing technique) can make the length of PCR products that can be sequenced by a sequencer longer than the maximum sequencing length of the sequencer whilst making full use of the characteristics of the second generation sequencing technique such as high throughput and low cost, thereby greatly broadening its applicable scope. In addition, the present invention also provides primer indexes for the PCR sequencing method and the use of the method in genotyping, particularly in HLA analysis, and also provides the PCR primers used, particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.

摘要翻译： 本发明提供了一种PCR测序方法，其中引物指数，DNA不完全剪切策略和第二代测序技术（配对终点测序技术）的组合可以使序列长度超过最大序列的PCR产物的序列长度 定序器的测序长度，同时充分利用第二代测序技术的特点，如高吞吐量和低成本，从而大大拓宽了其适用范围。 另外，本发明还提供了PCR测序方法的引物指标和基因分型方法的使用，特别是在HLA分析中，还提供了使用的PCR引物，特别是HLA-A，B，HLA- C和HLA-DQB1基因。

公开(公告)号：US09957564B2

公开(公告)日：2018-05-01

申请号：US13807660

申请日：2011-06-30

申请人： Jian Li ,  Shiping Chen ,  Xiandong Zhang ,  Ying Liu ,  Caifen Zhang ,  Tao Liu ,  Meiru Zhao

发明人： Jian Li ,  Shiping Chen ,  Xiandong Zhang ,  Ying Liu ,  Caifen Zhang ,  Tao Liu ,  Meiru Zhao

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  C12Q2525/191

摘要： The invention provides a PCR sequencing method, wherein the combination of primer indexes, DNA incomplete shearing strategy and the second generation sequencing technique (Paired-End sequencing technique) can make the length of PCR products that can be sequenced by a sequencer longer than the maximum sequencing length of the sequencer while making full use of the characteristics of the second generation sequencing technique such as high throughput and low cost, thereby greatly broadening its applicable scope. In addition, the present invention also provides primer indexes for the PCR sequencing method and the use of the method in genotyping, particularly in HLA analysis, and also provides the PCR primers used, particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.

公开(公告)号：US09493821B2

公开(公告)日：2016-11-15

申请号：US13989031

申请日：2011-09-21

申请人： Ye Du ,  Meiru Zhao ,  Ying Chen ,  Jinghua Wu ,  Geng Tian ,  Jun Wang

发明人： Ye Du ,  Meiru Zhao ,  Ying Chen ,  Jinghua Wu ,  Geng Tian ,  Jun Wang

IPC分类号： C12Q1/68 ,  C12N15/10 ,  C40B50/06

CPC分类号： C12Q1/683 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q1/6883 ,  C12Q2521/313 ,  C12Q2525/191 ,  C40B50/06

摘要： A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 45I; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library.

公开(公告)号：US09593387B2

公开(公告)日：2017-03-14

申请号：US13377116

申请日：2010-11-15

申请人： Xin Yi ,  Jiajia Xu ,  Xifang Nie ,  Meiru Zhao

发明人： Xin Yi ,  Jiajia Xu ,  Xifang Nie ,  Meiru Zhao

IPC分类号： C12Q1/16 ,  C12Q1/70 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/708 ,  C12Q1/6874

摘要： The present invention relates to a method for detecting Human Papilloma Virus (HPV), in particular, to a method for detecting HPV based on Solexa sequencing method.

摘要翻译： 本发明涉及一种用于检测人乳头状瘤病毒（HPV）的方法，特别涉及一种基于Solexa测序方法检测HPV的方法。

公开(公告)号：US20130123114A1

公开(公告)日：2013-05-16

申请号：US13377116

申请日：2010-11-15

申请人： Xin Yi ,  Jiajia Xu ,  Xifang Nie ,  Meiru Zhao

发明人： Xin Yi ,  Jiajia Xu ,  Xifang Nie ,  Meiru Zhao

IPC分类号： C12Q1/70

CPC分类号： C12Q1/708 ,  C12Q1/6874

摘要： The present invention relates to a method for detecting Human Papilloma Virus (HPV), in particular, to a method for detecting HPV based on Solexa sequencing method.

摘要翻译： 本发明涉及一种用于检测人乳头状瘤病毒（HPV）的方法，特别涉及一种基于Solexa测序方法检测HPV的方法。