公开(公告)号：US20100196930A1

公开(公告)日：2010-08-05

申请号：US12683527

申请日：2010-01-07

申请人： Jian-Shen Qi ,  Wensheng Lang ,  Margery A. Connelly

发明人： Jian-Shen Qi ,  Wensheng Lang ,  Margery A. Connelly

IPC分类号： G01N33/567

CPC分类号： G01N33/5038 ,  C12Q1/48 ,  G01N33/60 ,  G01N33/92

摘要： A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity.

摘要翻译： 用于鉴定调节酰基-CoA：二酰基甘油酰基转移酶的活性的化合物的方法包括以下步骤：在化合物的存在或不存在下使稳定同位素标记的脂肪酸与细胞接触，用异丙醇萃取细胞，并测定水平 的化合物存在或不存在下的稳定同位素标记的甘油三酸酯; 其中稳定同位素标记的甘油三酯水平的变化表明该化合物调节DGAT活性。

公开(公告)号：US09012159B2

公开(公告)日：2015-04-21

申请号：US12683527

申请日：2010-01-07

申请人： Jian-Shen Qi ,  Wensheng Lang ,  Margery A. Connelly

发明人： Jian-Shen Qi ,  Wensheng Lang ,  Margery A. Connelly

IPC分类号： G01N33/567 ,  G01N33/50 ,  C12Q1/48 ,  G01N33/60 ,  G01N33/92

CPC分类号： G01N33/5038 ,  C12Q1/48 ,  G01N33/60 ,  G01N33/92

摘要： A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity.

摘要翻译： 用于鉴定调节酰基-CoA：二酰基甘油酰基转移酶的活性的化合物的方法包括以下步骤：在化合物的存在或不存在下使稳定同位素标记的脂肪酸与细胞接触，用异丙醇萃取细胞，并测定水平 的化合物存在或不存在下的稳定同位素标记的甘油三酸酯; 其中稳定同位素标记的甘油三酯水平的变化表明该化合物调节DGAT活性。

公开(公告)号：US07452708B2

公开(公告)日：2008-11-18

申请号：US11516961

申请日：2006-09-07

申请人： Andrew Lawrence Darrow ,  Jian-Shen Qi ,  Cailin Chen ,  Patricia Andrade-Gordon

发明人： Andrew Lawrence Darrow ,  Jian-Shen Qi ,  Cailin Chen ,  Patricia Andrade-Gordon

IPC分类号： C12N9/64

CPC分类号： C07H21/04 ,  C12N9/6424 ,  C12Q1/37 ,  G01N2500/00

摘要： A novel human cDNA, termed PRSS11-L, was isolated which encodes a polypeptide that belongs to the S2/HtrA serine protease family. The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting. The full-length PRSS11-L cDNA, was cloned, expressed and purified. Proteolytic activity was demonstrated using the protein substrate casein. The isolated nucleic acid or polypeptide molecule of the invention can be used in detection assays, gene therapy, and screening assays.

摘要翻译： 分离出一种称为PRSS11-L的新型人类cDNA，其编码属于S2 / HtrA丝氨酸蛋白酶家族的多肽。 PRSS11-L mRNA通过多组织Northern印迹在全身的几种组织中广泛表达。 全长PRSS11-L cDNA被克隆，表达和纯化。 使用蛋白质底物酪蛋白证明蛋白水解活性。 本发明的分离的核酸或多肽分子可用于检测测定，基因治疗和筛选测定。