公开(公告)号：US20070202596A1

公开(公告)日：2007-08-30

申请号：US11710864

申请日：2007-02-26

申请人： Jim McWhir ,  Joseph Gold ,  J. Schiff

发明人： Jim McWhir ,  Joseph Gold ,  J. Schiff

IPC分类号： C12N5/08 ,  C12N5/00

CPC分类号： C12N9/1276 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0606 ,  C12N9/1048 ,  C12N2502/13 ,  C12N2510/00 ,  C12N2799/022 ,  C12Y204/01087

摘要： This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in expression of a cell-surface antigen that can be used to deplete the undifferentiated cells. Model effector sequences encode glycosyl transferases that synthesize carbohydrate xenoantigen or alloantigen, which can be used for immunoseparation or as a target for complement-mediated lysis. The differentiated cell populations produced are suitable for use in tissue regeneration and non-therapeutic applications such as drug screening.

摘要翻译： 本发明提供了一种用于从干细胞群体产生分化细胞的系统，用于需要相对均匀细胞群体的地方。 细胞含有在转录控制元件（例如TERT启动子）的控制下的效应基因，其导致基因在群体中相对未分化的细胞中表达。 效应基因的表达导致可用于消耗未分化细胞的细胞表面抗原的表达。 模型效应序列编码糖基转移酶，其合成碳水化合物异种抗原或同种异体抗原，其可用于免疫分离或作为补体介导的裂解的靶标。 产生的分化细胞群体适用于组织再生和非治疗应用，如药物筛选。

公开(公告)号：US06921665B2

公开(公告)日：2005-07-26

申请号：US09995419

申请日：2001-11-26

申请人： Jim McWhir ,  Joseph D. Gold ,  J. Michael Schiff

发明人： Jim McWhir ,  Joseph D. Gold ,  J. Michael Schiff

IPC分类号： A61K35/12 ,  A61K48/00 ,  C12N5/0735 ,  C12N9/10 ,  C12N15/54 ,  A01N1/00 ,  A01N1/02 ,  G01N33/53 ,  C12P1/00 ,  C12N15/63

CPC分类号： C12N9/1276 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0606 ,  C12N9/1048 ,  C12N2502/13 ,  C12N2510/00 ,  C12N2799/022 ,  C12Y204/01087

摘要： This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in expression of a cell-surface antigen that can be used to deplete the undifferentiated cells. Model effector sequences encode glycosyl transferases that synthesize carbohydrate xenoantigen or alloantigen, which can be used for immunoseparation or as a target for complement-mediated lysis. The differentiated cell populations produced are suitable for use in tissue regeneration and non-therapeutic applications such as drug screening.

摘要翻译： 本发明提供了一种用于从干细胞群体产生分化细胞的系统，用于需要相对均匀细胞群体的地方。 细胞含有在转录控制元件（例如TERT启动子）的控制下的效应基因，其导致基因在群体中相对未分化的细胞中表达。 效应基因的表达导致可用于消耗未分化细胞的细胞表面抗原的表达。 模型效应序列编码糖基转移酶，其合成碳水化合物异种抗原或同种异体抗原，其可用于免疫分离或作为补体介导的裂解的靶标。 产生的分化细胞群体适用于组织再生和非治疗应用，如药物筛选。