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    Selective antibody targeting of undifferentiated stem cells
    1.
    发明授权
    Selective antibody targeting of undifferentiated stem cells 失效
    未分化干细胞的选择性抗体靶向

    公开(公告)号：US06921665B2

    公开(公告)日：2005-07-26

    申请号：US09995419

    申请日：2001-11-26

    申请人： Jim McWhir ,  Joseph D. Gold ,  J. Michael Schiff

    发明人： Jim McWhir ,  Joseph D. Gold ,  J. Michael Schiff

    IPC分类号： A61K35/12 ,  A61K48/00 ,  C12N5/0735 ,  C12N9/10 ,  C12N15/54 ,  A01N1/00 ,  A01N1/02 ,  G01N33/53 ,  C12P1/00 ,  C12N15/63

    CPC分类号： C12N9/1276 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0606 ,  C12N9/1048 ,  C12N2502/13 ,  C12N2510/00 ,  C12N2799/022 ,  C12Y204/01087

    摘要： This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in expression of a cell-surface antigen that can be used to deplete the undifferentiated cells. Model effector sequences encode glycosyl transferases that synthesize carbohydrate xenoantigen or alloantigen, which can be used for immunoseparation or as a target for complement-mediated lysis. The differentiated cell populations produced are suitable for use in tissue regeneration and non-therapeutic applications such as drug screening.

    摘要翻译： 本发明提供了一种用于从干细胞群体产生分化细胞的系统，用于需要相对均匀细胞群体的地方。 细胞含有在转录控制元件（例如TERT启动子）的控制下的效应基因，其导致基因在群体中相对未分化的细胞中表达。 效应基因的表达导致可用于消耗未分化细胞的细胞表面抗原的表达。 模型效应序列编码糖基转移酶，其合成碳水化合物异种抗原或同种异体抗原，其可用于免疫分离或作为补体介导的裂解的靶标。 产生的分化细胞群体适用于组织再生和非治疗应用，如药物筛选。

    Marker system for preparing and characterizing high-quality human embryonic stem cells
    6.
    发明授权
    Marker system for preparing and characterizing high-quality human embryonic stem cells 有权
    用于制备和表征高质量人类胚胎干细胞的标记系统

    公开(公告)号：US07153650B2

    公开(公告)日：2006-12-26

    申请号：US10389431

    申请日：2003-03-13

    申请人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok

    发明人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5005

    摘要： This disclosure provides a system for qualifying embryonic stem cells intended for human therapy. A large-scale sequencing project has identified important markers that are characteristic of undifferentiated pluripotent cells. Combinations of these markers can be used to validate the self-renewing capacity of ES cells, and their ability to differentiate into tissue types suitable for regenerative medicine. The marker system of this invention has been used to screen feeder cells, media additives, and culture conditions that promote proliferation of stem cells without differentiation. A culture system optimized by following these markers is suitable for rapid expansion of undifferentiated cells from existing lines, or the derivation of new lines that are equally apposite for clinical use.

    摘要翻译： 本公开提供了用于限定用于人类治疗的胚胎干细胞的系统。 大规模测序项目确定了未分化多能细胞特征的重要标志物。 这些标记的组合可以用于验证ES细胞的自我更新能力，以及它们分化成适合再生医学的组织类型的能力。 本发明的标记系统已用于筛选不分化促进干细胞增殖的饲养细胞，培养基添加剂和培养条件。 通过遵循这些标记优化的培养系统适用于从现有品系中未分化细胞的快速扩增，或适用于临床应用的新品系的衍生。