公开(公告)号：US08653252B2

公开(公告)日：2014-02-18

申请号：US10550152

申请日：2004-03-22

申请人： Joachim Elmén ,  Claes Wahlestedt ,  Zicai Liang ,  Anders Malling Sørensen ,  Henrik Ørum ,  Troels Koch

发明人： Joachim Elmén ,  Claes Wahlestedt ,  Zicai Liang ,  Anders Malling Sørensen ,  Henrik Ørum ,  Troels Koch

IPC分类号： C07H21/04 ,  C12N15/113

CPC分类号： C12N15/1131 ,  A61K38/00 ,  A61K38/09 ,  A61K38/2013 ,  A61K38/212 ,  A61K38/31 ,  C12N15/111 ,  C12N15/1135 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3233 ,  C12N2310/3341 ,  C12N2310/53 ,  C12N2320/51 ,  C12N2330/30 ,  Y02A50/465 ,  A61K2300/00

摘要： The present invention is directed to novel double-stranded short interfering (siRNA) analogues comprising locked nucleic acid (LNA) monomers. Such compounds induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). The compounds disclosed herein has improved properties compared to non-modified siRNAs and may, accordingly, be useful as therapeutic agents, e.g., in the treatment of various cancer forms. More particularly, the present invention is directed to siRNA analogues comprising a sense strand and an antisense strand, wherein each strand comprises 12-35 nucleotides and wherein the siRNA analogues comprise at least one locked nucleic acid (LNA) monomer.

摘要翻译： 本发明涉及包含锁定核酸（LNA）单体的新型双链短干扰（siRNA）类似物。 这种化合物通过称为RNA干扰（RNAi）的方法在许多生物体中诱导序列特异性的转录后基因沉默。 本文公开的化合物与未修饰的siRNA相比具有改善的性质，因此可用作治疗剂，例如用于治疗各种癌症形式。 更具体地，本发明涉及包含有义链和反义链的siRNA类似物，其中每条链包含12-35个核苷酸，并且其中siRNA类似物包含至少一个锁定核酸（LNA）单体。

公开(公告)号：US09574189B2

公开(公告)日：2017-02-21

申请号：US12095778

申请日：2006-12-01

申请人： Thomas Franch ,  Mikkel Dybro Lundorf ,  Søren Nyboe Jacobsen ,  Eva Kampmann Olsen ,  Anne Lee Andersen ,  Anette Holtmann ,  Anders Holm Hansen ,  Anders Malling Sørensen ,  Anne Goldbech ,  Daen de Leon ,  Ditte Kievsmose Kaldor ,  Frank Abildgaard Sløk ,  Gitte Nystrup Husemoen ,  Johannes Dolberg ,  Kim Birkebæ Jensen ,  Lene Pedersen ,  Mads Nørregaard-Madsen ,  Michael Anders Godskesen ,  Sanne Schrøder Glad ,  Søren Neve ,  Thomas Thisted ,  Tine Titilola Akinleminu Kronborg ,  Christian Klarner Sams ,  Jakob Felding ,  Per-Ola Freskgård ,  Alex Haahr Gouliaev ,  Henrik Pedersen

发明人： Thomas Franch ,  Mikkel Dybro Lundorf ,  Søren Nyboe Jacobsen ,  Eva Kampmann Olsen ,  Anne Lee Andersen ,  Anette Holtmann ,  Anders Holm Hansen ,  Anders Malling Sørensen ,  Anne Goldbech ,  Daen de Leon ,  Ditte Kievsmose Kaldor ,  Frank Abildgaard Sløk ,  Gitte Nystrup Husemoen ,  Johannes Dolberg ,  Kim Birkebæ Jensen ,  Lene Pedersen ,  Mads Nørregaard-Madsen ,  Michael Anders Godskesen ,  Sanne Schrøder Glad ,  Søren Neve ,  Thomas Thisted ,  Tine Titilola Akinleminu Kronborg ,  Christian Klarner Sams ,  Jakob Felding ,  Per-Ola Freskgård ,  Alex Haahr Gouliaev ,  Henrik Pedersen

IPC分类号： C12N15/10 ,  C40B40/08 ,  C40B50/04 ,  C40B50/06

CPC分类号： C12N15/1068 ,  C12N15/1089 ,  C40B40/08 ,  C40B50/04 ,  C40B50/06

摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).

摘要翻译： 公开了一种获得包含与单链标识符寡核苷酸连接的分子的双功能复合物的方法，其中包含化学反应位点的新生双功能复合物和用于酶标记添加的引发位点是a）在化学反应位点与 一种或多种反应物，和b）在起始位点用一种或多种标记反应物的标签进行酶促反应。