公开(公告)号：US5624829A

公开(公告)日：1997-04-29

申请号：US993785

申请日：1992-12-21

申请人： Johan P. M. Sanders ,  Johannes A. van den Berg ,  Peter M. Andreoli ,  Yvonne J. Vos ,  Jan H. van Ee ,  Leo J. S. M. Mulleners

发明人： Johan P. M. Sanders ,  Johannes A. van den Berg ,  Peter M. Andreoli ,  Yvonne J. Vos ,  Jan H. van Ee ,  Leo J. S. M. Mulleners

IPC分类号： C12N9/28 ,  C12N9/54 ,  C12N9/64 ,  C12N15/63 ,  C12N15/75 ,  C12N15/03 ,  C12N1/21

CPC分类号： C12N9/2417 ,  C12N15/63 ,  C12N15/75 ,  C12N9/54 ,  C12N9/6481 ,  C12N9/6483 ,  C12Y304/23004

摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

摘要翻译： 提供新颖的方法和新型工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有表达目的多肽的基因的克隆载体引入相容宿主体内。 选择通过将载体整合到宿主细胞染色体中，以稳定的方式携带引入载体的转化宿主。 实现含有目标基因的载体的有效转移，所得到的工业菌株转化体是所需多肽产物的有效稳定的生产者。

公开(公告)号：US4859596A

公开(公告)日：1989-08-22

申请号：US572414

申请日：1984-01-19

申请人： Cornelis P. Hollenberg ,  Sunil Das ,  Albert De Leeuw ,  Johannes A. van den Berg

发明人： Cornelis P. Hollenberg ,  Sunil Das ,  Albert De Leeuw ,  Johannes A. van den Berg

IPC分类号： C12N15/00 ,  A61K35/74 ,  A61K38/00 ,  C07K14/43 ,  C12N1/16 ,  C12N1/19 ,  C12N9/34 ,  C12N9/64 ,  C12N15/09 ,  C12N15/68 ,  C12N15/81 ,  C12N15/90 ,  C12P21/00 ,  C12P21/02 ,  C12R1/645

CPC分类号： C12N9/2428 ,  C07K14/43 ,  C12N15/68 ,  C12N15/815 ,  C12N15/90 ,  C12N9/6481 ,  C07K2319/036

摘要： A new cloning system is described capable of expressing genetic material derived from recombinant DNA material, which comprises a yeast of the genus Kluyveromyces as a host. Suitable vectors are e.g. vectors containing autonomously replicating sequences (ARS) and vectors containing homologous Kluyveromyces DNA acting as a site for recombination with the host chromosome. New and preferred vectors are those containing ARS sequences originating from Kluyveromyces (KARS vectors). The genetically engineered new strains of Kluyveromyces produce, inter alia, lactase and chymosin.