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公开(公告)号：US5238833A
公开(公告)日：1993-08-24
申请号：US701596
申请日：1991-05-13
申请人： Johan P. M. Sanders , Johannes A. Van Den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. Van EE , Leo J. S. M. Mulleners
发明人： Johan P. M. Sanders , Johannes A. Van Den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. Van EE , Leo J. S. M. Mulleners
CPC分类号： C12N9/2417 , C12N15/63 , C12N15/75 , C12N9/54 , C12N9/6481
摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
摘要翻译： 提供新颖的方法和新型工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有表达目的多肽的基因的克隆载体引入相容宿主体内。 选择通过将载体整合入宿主细胞染色体以稳定方式携带引入载体的转化宿主实现了含有目的基因的载体的有效转移，所得到的工业菌株转化体是有效的，所需多肽的稳定生产者 产品。
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2.发明授权Transformed industrial bacillus strains and methods for making and using them 失效转化的工业芽孢杆菌菌株及其制备和使用方法
公开(公告)号：US5624829A
公开(公告)日：1997-04-29
申请号：US993785
申请日：1992-12-21
申请人： Johan P. M. Sanders , Johannes A. van den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. van Ee , Leo J. S. M. Mulleners
发明人： Johan P. M. Sanders , Johannes A. van den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. van Ee , Leo J. S. M. Mulleners
CPC分类号： C12N9/2417 , C12N15/63 , C12N15/75 , C12N9/54 , C12N9/6481 , C12N9/6483 , C12Y304/23004
摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
摘要翻译： 提供新颖的方法和新型工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有表达目的多肽的基因的克隆载体引入相容宿主体内。 选择通过将载体整合到宿主细胞染色体中，以稳定的方式携带引入载体的转化宿主。 实现含有目标基因的载体的有效转移，所得到的工业菌株转化体是所需多肽产物的有效稳定的生产者。
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公开(公告)号：US4430434A
公开(公告)日：1984-02-07
申请号：US147483
申请日：1980-05-07
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/75 , C12P19/34 , C12R1/07 , C12R1/085 , C12R1/19 , C12R1/44 , C12R1/445 , C12N1/00 , C12N15/00 , C12P21/00
CPC分类号： C12N15/75
摘要： Plasmid conferring resistance to streptomycin (Sm.sup.R) and neomycin (Neo.sup.R) upon its host (e.g. a Bacillus, Staphylococcus or Escherichia coli) and which after having taken up a foreign DNA fragment at one of its restriction sites with loss of its Sm.sup.R or Neo.sup.R phenotype, is still capable of replicating and expressing genetic information in its host.
摘要翻译： 其质粒赋予其宿主（例如芽孢杆菌属，葡萄球菌属或大肠杆菌）上的链霉素（SmR）和新霉素（NeoR）的抗性，并且在其限制性位点之一吸收了外来DNA片段后失去其SmR或NeoR表型 ，仍然能够在其宿主中复制和表达遗传信息。
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公开(公告)号：US4460688A
公开(公告)日：1984-07-17
申请号：US332958
申请日：1981-12-21
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/75 , C12P19/34 , C12R1/07 , C12R1/085 , C12R1/19 , C12R1/44 , C12R1/445 , C12N15/00 , C12N1/00 , C12P21/00 , C12P21/02 , C12R1/125
CPC分类号： C12N15/75
摘要： Plasmid conferring resistance to streptomycin (Sm.sup.R) and neomycin (Neo.sup.R) upon its host (e.g. a Bacillus, Staphylococcus or Escherichia coli) and which after having taken up a foreign DNA fragment at one of its restriction sites with loss of its Sm.sup.R or Neo.sup.R phenotype, is still capable of replicating and expressing genetic information in its host.
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5.发明授权
公开(公告)号：US4677198A
公开(公告)日：1987-06-30
申请号：US671666
申请日：1984-11-15
摘要： Process for the preparation of oligosaccharides-containing products from biomass involving the use of hydrochloric acid for partly hydrolyzing biomass to easily fermentable oligosaccharides-containing products and easy recovery of the hydrochloric acid and the oligosaccharides containing products. The oligosaccharides-containing products themselves and use of the latter for the preparation of useful products like ethanol and penicillin by fermentation.
摘要翻译： 用于从生物质制备含寡糖的产品的方法，其涉及使用盐酸部分水解生物质以容易发酵的含寡糖产物，并容易地回收盐酸和含寡糖的产品。 含寡糖的产品本身和后者用于通过发酵制备有用的产品如乙醇和青霉素。
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