公开(公告)号：US09127302B2

公开(公告)日：2015-09-08

申请号：US12067532

申请日：2006-09-20

申请人： John A Verrant ,  Arjan G. J. Tibbe ,  Brad Foulk ,  Joost F. Swennenhuis ,  Leon W. M. M. Terstappen ,  Mark Carle Connelly ,  Michael T. Kagan

发明人： John A Verrant ,  Arjan G. J. Tibbe ,  Brad Foulk ,  Joost F. Swennenhuis ,  Leon W. M. M. Terstappen ,  Mark Carle Connelly ,  Michael T. Kagan

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  A61K39/395 ,  C12Q1/34

CPC分类号： C12Q1/6886 ,  C12Q1/34 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2533/101 ,  C12Q2531/113 ,  C12Q2521/301 ,  C12Q2563/131 ,  C12Q2563/103

摘要： The invention relates generally to the field of identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.

摘要翻译： 本发明一般涉及DNA序列，基因或染色体的鉴定领域。 提供了获得独特序列DNA探针的方法和组成。 组合物包含根据本发明描述的方法从其中排除重复序列的任何含有唯一序列的双链DNA。 本发明还涉及在免疫磁性选择和荧光标记之后鉴定的细胞的保存以进一步询问感兴趣的细胞。 此外，本发明涉及在免疫磁性选择和荧光标记之后鉴定的细胞的遗传分析。