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    RAPID, SEMI-AUTOMATED METHOD TO DETECT RESPIRATORY VIRUS INFECTED CELLS IN DIRECT SPECIMENS
    6.
    发明申请
    RAPID, SEMI-AUTOMATED METHOD TO DETECT RESPIRATORY VIRUS INFECTED CELLS IN DIRECT SPECIMENS 审中-公开
    RAPID，半自动检测直接样本中呼吸道感染细胞的方法

    公开(公告)号：US20120202189A1

    公开(公告)日：2012-08-09

    申请号：US12450846

    申请日：2008-04-16

    申请人： Mark Carle Connelly ,  Frank A.W. Coumans ,  Jimmy Page ,  Galla Chandra Rao ,  Joseph Jollick, JR.

    发明人： Mark Carle Connelly ,  Frank A.W. Coumans ,  Jimmy Page ,  Galla Chandra Rao ,  Joseph Jollick, JR.

    IPC分类号： G01N21/17

    CPC分类号： G01N33/56983 ,  G01N33/5091 ,  G01N33/54326 ,  G01N2333/11

    摘要： A novel cytometer system, methods and algorithms which provide a rugged, affordable and easy-to-use device in remote locations. All cells in a biological sample are fluorescently labeled, with target cells also magnetically labeled. Non-magnetically labeled cells are imaged for viability. Labeled sample is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells for observation. A LED illuminates the cells and a CCO camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a cell count that can be related to the target cell concentration of the original sample.

    摘要翻译： 一种新颖的细胞仪系统，方法和算法，可在远程位置提供坚固耐用，价格实惠且易于使用的设备。 生物样品中的所有细胞都被荧光标记，靶细胞也被磁性标记。 对非磁性标记的细胞进行成像。 将标记的样品放置在两个楔形磁体之间，以选择性地移动磁性标记的细胞用于观察。 LED照亮细胞，CCO相机拍摄由靶细胞发出的荧光的图像。 用新颖算法进行的图像分析提供了可以与原始样品的靶细胞浓度相关的细胞计数。

    High sensitivity multiparameter method for rare event analysis in a biological sample
    7.
    发明申请
    High sensitivity multiparameter method for rare event analysis in a biological sample 有权
    用于生物样品中罕见事件分析的高灵敏度多参数方法

    公开(公告)号：US20090029378A1

    公开(公告)日：2009-01-29

    申请号：US12181399

    申请日：2008-07-29

    申请人： Mark Carle Connelly ,  Frank Coumans ,  Steve Gross ,  Mike Kelly

    发明人： Mark Carle Connelly ,  Frank Coumans ,  Steve Gross ,  Mike Kelly

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/569 ,  G01N33/57488 ,  G01N33/582 ,  G01N2021/6441

    摘要： A sample of blood containing CTCs, or other cells of interest, is stained with fluorescent markers for image analysis and scanned to identify the presence and location within the cartridge of target cells or subcellular elements. A sample containing desired target cells or subcellular elements is then further processed, in part by photobleaching the sample, so that those same targets may be re-analyzed with additional biomarkers conjugated to the same or different fluorochromes using the same imaging criteria that were used for the initial analysis. The present invention has applications with targets such as circulating epithelial, cells, circulating tumor cells, circulating endothelial cells, leukocytes, lymphocyte subsets, cells containing an organelle or receptor of interest, cellular debris, disrupted cells and their debris, or any other formed element that might be captured and imaged. The invention provides a means to further interrogate individual targets of interest, especially when coupled with genetic analysis such as FISH.

    摘要翻译： 含有CTC或其他感兴趣的细胞的血液样品用用于图像分析的荧光标记染色并扫描以鉴定靶细胞或亚细胞元件的盒内的存在和位置。 然后进一步处理含有所需靶细胞或亚细胞元件的样品，部分通过光漂白样品进行处理，使得可以使用与用于相同或不同荧光染料的相同成像标准的附加生物标志物重新分析相同的靶标 初步分析。 本发明具有诸如循环上皮细胞，细胞，循环肿瘤细胞，循环内皮细胞，白细胞，淋巴细胞亚群，含有感兴趣的细胞器或受体的细胞，细胞碎片，破裂的细胞及其碎片或任何其它形成的元件 这可能被捕获和成像。 本发明提供了进一步询问感兴趣的个体目标的方法，特别是当与FISH等遗传分析相结合时。

    METHODS AND KITS FOR THE DETECTION OF CIRCULATING TUMOR CELLS IN PANCREATIC PATIENTS USING POLYSPECIFIC CAPTURE AND COCKTAIL DETECTION REAGENTS
    10.
    发明申请
    METHODS AND KITS FOR THE DETECTION OF CIRCULATING TUMOR CELLS IN PANCREATIC PATIENTS USING POLYSPECIFIC CAPTURE AND COCKTAIL DETECTION REAGENTS 审中-公开
    使用聚合捕获剂和蛋白质检测试剂检测胰腺癌患者循环肿瘤细胞的方法和试剂盒

    公开(公告)号：US20120094275A1

    公开(公告)日：2012-04-19

    申请号：US13272769

    申请日：2011-10-13

    申请人： Galla Chandra Rao ,  Mark Carle Connelly

    发明人： Galla Chandra Rao ,  Mark Carle Connelly

    IPC分类号： C12Q1/70 ,  G01N33/554 ,  G01N33/566

    CPC分类号： G01N33/57492 ,  G01N33/54333

    摘要： A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric or image cytometry to detect, enumerate and characterize carcinoma cells in the blood. The present invention incorporates the conjugation of different antibodies to the same ferrofluid. This has the effect of making the ferrofluid polyspecific with respect to the antigens that the ferrofluid will bind. The multiple antibodies present on the same ferrofluid do not appear to block or otherwise interfere with each other. Such ferrofluids have the highly desirable effect of being able to bind specifically to more than one type of cell. The assay is especially useful to enable the capture of CTCs that have low EpCAM expression, but high expression of other tumor markers; Accordingly, the assay facilitates the biological characterization and staging of carcinoma cells.

    摘要翻译： 公开了一种高度敏感的测定法，其将免疫磁性富集与多参数流式细胞术或图像细胞计数法结合，以检测，计数和表征血液中的癌细胞。 本发明包含不同抗体与相同铁磁流体的缀合。 这具有使铁磁流体多特异性相对于铁磁流体将结合的抗原的效果。 存在于相同铁磁流体上的多种抗体似乎并不阻碍或以其他方式相互干扰。 这种铁磁流体具有能够特异性结合多于一种类型的电池的非常理想的效果。 该测定特别有助于捕获具有低EpCAM表达但其他肿瘤标志物的高表达的CTC; 因此，该测定促进了癌细胞的生物学表征和分期。