公开(公告)号：US4581222A

公开(公告)日：1986-04-08

申请号：US465360

申请日：1983-02-09

申请人： John D. Baldeschwieler ,  Ronald C. Gamble ,  Albert M. Lin ,  George W. Tin

发明人： John D. Baldeschwieler ,  Ronald C. Gamble ,  Albert M. Lin ,  George W. Tin

IPC分类号： G01N33/534 ,  A61K43/00 ,  G01T1/00

CPC分类号： G01N33/534 ,  Y10S436/804 ,  Y10S436/827

摘要： An immunoassay method for detection of antigen is disclosed. The method employs complement mediated lysis of vesicles loaded with In-111 or other gamma-emitting cation, and quantitative detection of the lysis by gamma-ray perturbed angular correlation (PAC) spectroscopy. The vesicles are labeled with a substance competitive to the antigen to be measured, and the concentration of the antigen in the sample measured by assessing the diminution in lysis due to the presence of the competing antigen.The method may also be used to assess the immunologic competence of a subject by injecting suitably sensitized vesicles and monitoring the in vivo lysis pattern by (PAC).

摘要翻译： 公开了一种用于检测抗原的免疫测定方法。 该方法使用负载有In-111或其他γ发射阳离子的囊泡的补体介导的裂解，以及通过γ射线扰动角度相关（PAC）光谱定量检测裂解。 囊泡用与要测量的抗原竞争的物质标记，并且通过评估由于竞争性抗原的存在引起的裂解减少来测量样品中抗原的浓度。 该方法还可以用于通过注射适当敏化的囊泡和通过（PAC）监测体内裂解模式来评估受试者的免疫能力。

公开(公告)号：US4497791A

公开(公告)日：1985-02-05

申请号：US465502

申请日：1983-02-10

申请人： Ronald C. Gamble ,  George W. Tin ,  Lawrence E. Williams

发明人： Ronald C. Gamble ,  George W. Tin ,  Lawrence E. Williams

IPC分类号： G01N33/60 ,  A61K9/127 ,  A61K51/00 ,  A61K51/12 ,  C12N5/06 ,  C12Q1/00 ,  A61K43/00 ,  A61K49/00

CPC分类号： A61K9/1272 ,  A61K51/1203 ,  A61K51/1227 ,  A61K51/1234 ,  A61K2123/00

摘要： Described herein is a process for labeling leukocytes and other phagocytic cells with labeled micellular particles involving incubating the cells with the micellular particles. Also described is a process for detecting the locus of an infection by administering to a subject leukocytes radiolabeled by incubation with labeled micellular particles followed by scanning the subject to detect the locus of radiation emitted by the particles.

摘要翻译： 本文描述的是用标记的微胶囊颗粒标记白细胞和其它吞噬细胞的方法，包括将细胞与微胶粒颗粒一起孵育。 还描述了通过向受试者进行放射性标记的白细胞来检测感染的轨迹的方法，所述白细胞通过与标记的微粒颗粒孵育，随后扫描受试者以检测由颗粒发射的辐射的轨迹。

公开(公告)号：US06274115B1

公开(公告)日：2001-08-14

申请号：US08483494

申请日：1995-06-07

申请人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

发明人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

IPC分类号： A61K5100

CPC分类号： A61K9/1271 ,  A61K51/1234 ,  A61K2121/00 ,  A61K2123/00 ,  Y10S436/829

摘要： Micellular particles such as small unilamellar vesicles of less than 2000 A° loaded with 111In are administered to BALB/c mice in which EMT6 tumors had been induced. Whole body scintographs of the mice to which either neutral or positively or negatively charged vesicles had been administered show a substantial quantity of the vesicle entrapped 111In localized in the tumor. Blocking of macrophages in the liver and spleen by first administering unlabeled, aminomannose substituted vesicles before administration of the labeled vesicles increases uptake of the 111In labeled vesicles in the tumor.

摘要翻译： 将具有小于2000A的小单层囊泡的胶束颗粒（装载有111In）施用于已经诱导EMT6肿瘤的BALB / c小鼠。 已经施用中性或正性或带负电荷的小泡的小鼠的全身阴影图显示了定量存在于肿瘤中的大量囊泡111I。 在施用标记的囊泡之前首先施用未标记的氨基甘露糖糖溶液取代的囊泡，在肝脏和脾脏中阻断巨噬细胞增加肿瘤中111I标记的囊泡的摄取

公开(公告)号：US5435989A

公开(公告)日：1995-07-25

申请号：US663503

申请日：1984-10-22

申请人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

发明人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

IPC分类号： A61K9/127 ,  A61K9/133 ,  A61K51/12

CPC分类号： A61K9/1271 ,  A61K51/1234 ,  A61K2121/00 ,  A61K2123/00 ,  Y10S436/829

摘要： Micellular particles such as small unilamellar vesicles of less than 2000 .ANG. loaded with .sup.111 In are administered to BALB/c mice in which EMT6 tumors had been induced. Whole body scintographs of the mice to which either neutral or positively or negatively charged vesicles had been administered show a substantial quantity of the vesicle entrapped .sup.111 In localized in the tumor. Blocking of macrophages in the liver and spleen by first administering unlabeled, aminomannose substituted vesicles before administration of the labeled vesicles increases uptake of the .sup.111 In labeled vesicles in the tumor.

摘要翻译： 将具有小于2000个ANGSTROM的小单层囊泡的胶束颗粒施用于已经诱导EMT6肿瘤的BALB / c小鼠。 已经施用中性或正性或带负电荷的小泡的小鼠的全身阴影图显示了定量存在于肿瘤中的大量囊泡111I。 在施用标记的囊泡之前首先施用未标记的氨基甘露糖取代的囊泡，在肝脏和脾脏中阻断巨噬细胞增加了肿瘤中111I标记的囊泡的摄取。

公开(公告)号：US5186941A

公开(公告)日：1993-02-16

申请号：US794777

申请日：1991-11-18

申请人： Richard A. Callahan ,  George W. Tin

发明人： Richard A. Callahan ,  George W. Tin

IPC分类号： A61K9/127

CPC分类号： A61K9/127 ,  Y10S514/823 ,  Y10S514/885 ,  Y10S514/963 ,  Y10T428/2984

摘要： Disclosed herein are vesicle compositions for controlled sustained release of an encapsulated therapeutic agent after parenteral administration. By adjusting the osmolarity between the suspending solution and the solution within the vesicles by adjusting the concentration of the suspending solution, the rate of release of the therapeutic agent after parenteral administration can be varied. The compositions can be effectively administered by intramuscular, subcutaneous injection or other means.

摘要翻译： 本文公开了用于肠胃外给药后封装的治疗剂的受控持续释放的泡囊组合物。 通过调整悬浮液的浓度来调节悬浮液与囊泡内的溶液的渗透压，可以改变肠胃外给药后治疗剂的释放速度。 可以通过肌内，皮下注射或其它手段有效地施用组合物。

公开(公告)号：US5441745A

公开(公告)日：1995-08-15

申请号：US663550

申请日：1984-10-22

申请人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

发明人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

IPC分类号： A61K9/127 ,  A61K9/133 ,  A61K51/12

CPC分类号： A61K9/1271 ,  A61K51/1234 ,  A61K2121/00 ,  A61K2123/00 ,  Y10S436/829

摘要： A method is provided for delivering micellular particles containing chemotherapeutic agents and a marker to tumors within a body for the diagnosis and treatment of such tumors. The micellular particles are small, less than 2000 .ANG. and incorporate pure, neutral phospholipid molecules in their external surface. Enhanced delivery of the micellular particles containing marker and chemotherapeutic agents may be achieved by introducing an initial group of positively charged micellular particles to block the reticuloendothelial cells present in the body.

摘要翻译： 提供了一种用于将包含化学治疗剂的微粒颗粒和用于诊断和治疗这样的肿瘤的体内的肿瘤的标记递送到该体内的方法。 胶束颗粒小，小于2000 ANGSTROM，并在其外表面掺入纯中性磷脂分子。 通过引入带正电荷的微胶粒的初始组来阻止存在于体内的网状内皮细胞，可以实现含有标记物和化学治疗剂的胶束颗粒的增强递送。

公开(公告)号：US5008109A

公开(公告)日：1991-04-16

申请号：US257155

申请日：1988-10-11

申请人： George W. Tin

发明人： George W. Tin

IPC分类号： A61K9/127 ,  A61K51/12

CPC分类号： A61K9/127 ,  A61K51/1227 ,  A61K2123/00 ,  Y10S436/829 ,  Y10S514/937 ,  Y10S514/944 ,  Y10S514/963 ,  Y10S514/965 ,  Y10T428/2984

摘要： The present invention relates to a process of stabilizing micellular particles such as vesicles and increasing the shelf life by suspending the particles in a polymeric gel matrix. The invention also relates to such particles suspended in the gel matrix with a protective gel surface thereabout which is capable of becoming fluid and converting the protective surface of an aqueous suspension.

摘要翻译： 本发明涉及通过将颗粒悬浮在聚合物凝胶基质中来稳定诸如囊泡的胶束颗粒并增加保存期限的方法。 本发明还涉及悬浮在凝胶基质中的这样的颗粒，其上具有保护性凝胶表面，其能够变成流体并转化水性悬浮液的保护表面。