公开(公告)号：US09481880B2

公开(公告)日：2016-11-01

申请号：US12598414

申请日：2008-05-01

申请人： John I. Glass ,  Nina Alperovich ,  Clyde A. Hutchison, III ,  Carole Lartigue ,  Charles E. Merryman ,  Sanjay Vashee ,  J. Craig Venter

发明人： John I. Glass ,  Nina Alperovich ,  Clyde A. Hutchison, III ,  Carole Lartigue ,  Charles E. Merryman ,  Sanjay Vashee ,  J. Craig Venter

IPC分类号： C12N15/10 ,  C12N15/74

CPC分类号： C12N15/10 ,  C12N15/1079 ,  C12N15/74

摘要： The presently disclosed invention relates to methods of installing a genome isolated from one species (the donor) into suitably prepared cells of a second species (the recipient). Introduction of the donor genetic material into the recipient host cell effectively converts the recipient host cell into a new cell that, as a result of the operation of the donated genetic material, is functionally classified as belonging to the genus and species of the donor genetic material.

摘要翻译： 本公开的本发明涉及将从一个物种（供体）分离的基因组安装到适当制备的第二物种（受体）的细胞中的方法。 将供体遗传物质引入受体宿主细胞有效地将受体宿主细胞转化为新的细胞，由于所捐赠的遗传物质的操作，功能上被归类为供体遗传物质的属和物种 。

公开(公告)号：US20110045592A1

公开(公告)日：2011-02-24

申请号：US12598414

申请日：2008-05-01

申请人： John I. Glass ,  Nina Alperovich ,  Clyde A. Hutchison, III ,  Carole Lartigue ,  Charles E. Merryman ,  Sanjay Vashee ,  J. Craig Venter

发明人： John I. Glass ,  Nina Alperovich ,  Clyde A. Hutchison, III ,  Carole Lartigue ,  Charles E. Merryman ,  Sanjay Vashee ,  J. Craig Venter

IPC分类号： C12N15/74

CPC分类号： C12N15/10 ,  C12N15/1079 ,  C12N15/74

摘要： The presently disclosed invention relates to methods of installing a genome isolated from one species (the donor) into suitably prepared cells of a second species (the recipient). Introduction of the donor genetic material into the recipient host cell effectively converts the recipient host cell into a new cell that, as a result of the operation of the donated genetic material, is functionally classified as belonging to the genus and species of the donor genetic material.

摘要翻译： 本公开的本发明涉及将从一个物种（供体）分离的基因组安装到适当制备的第二物种（受体）的细胞中的方法。 将供体遗传物质引入受体宿主细胞有效地将受体宿主细胞转化为新的细胞，由于所捐赠的遗传物质的操作，功能上被归类为供体遗传物质的属和物种 。

公开(公告)号：US20070122826A1

公开(公告)日：2007-05-31

申请号：US11546364

申请日：2006-10-12

申请人： John Glass ,  Hamilton Smith ,  Clyde Hutchison ,  Nina Alperovich ,  Nacyra Assad-Garcia

发明人： John Glass ,  Hamilton Smith ,  Clyde Hutchison ,  Nina Alperovich ,  Nacyra Assad-Garcia

IPC分类号： C40B30/06 ,  C40B40/10 ,  C07H21/04 ,  C12P7/06 ,  C12P1/06 ,  C12N9/02 ,  C12N1/21

CPC分类号： C12N1/20 ,  C07K14/195 ,  C07K14/30 ,  C12N9/16 ,  C12P3/00 ,  C12P7/06 ,  C12R1/35 ,  C12Y301/04046 ,  Y02E50/17

摘要： The present invention relates, e.g., to a minimal set of protein-coding genes which provides the information required for replication of a free-living organism in a rich bacterial culture medium, wherein (1) the gene set does not comprise the 101 genes listed in Table 2; and/or wherein (2) the gene set comprises the 381 protein-coding genes listed in Table 3 and, optionally, one of more of: a set of three genes encoding ABC transporters for phosphate import (genes MG410, MG411 and MG412; or genes MG289, MG290 and MG291); the lipoprotein-encoding gene MG185 or MG260; and/or the glycerophosphoryl diester phosphodiesterase gene MG293 or MG385.

摘要翻译： 本发明涉及例如提供在富细菌培养基中复制自由活体的所需信息的蛋白质编码基因的最小集合，其中（1）基因组不包括所列出的101个基因 在表2中; 和/或其中（2）基因组包含表3中列出的381个蛋白质编码基因，以及任选的一个以下的一个：编码用于磷酸酯进口的ABC转运蛋白的三个基因（基因MG410，MG411和MG412;或 基因MG289，MG290和MG291）; 编码脂蛋白的基因MG185或MG260; 和/或甘油磷酸二酯磷酸二酯酶基因MG293或MG385。