Method for assessing the fertility of male mammals
    5.
    发明授权
    Method for assessing the fertility of male mammals 失效
    评估雄性哺乳动物生殖力的方法

    公开(公告)号:US4767703A

    公开(公告)日:1988-08-30

    申请号:US596327

    申请日:1984-04-03

    IPC分类号: G01N33/50 C12Q1/02

    摘要: A method for evaluating the fertility of a male mammal. A semen sample is obtained from the individual to be tested. The sperm is separated from the seminal plasma of the semen sample. At least one test portion and a control portion of the sperm are isolated. The sperm portions are incubated, the test portion being exposed to a glycosaminoglycan in a concentration effective to induce an acrosome reaction in sperm. A representative sample of incubated sperm from each portion is then counted by means of observation by light microscopy to measure the increase in acrosome reaction in the test portions as compared to the control portion. A kit is provided for the convenient performance of the method. The kit includes a supply of sterile liquid culture medium, sterile closable culture vessels, and a supply of selected, sterile GAG meterable substantially aseptically in known amounts into each culture vessel used as a test vessel.

    摘要翻译: 一种评估雄性哺乳动物生育能力的方法。 从要测试的个体获得精液样品。 精子与精液样品的精液等离子体分离。 分离至少一个测试部分和精子的对照部分。 将精子部分温育,将测试部分暴露于浓度为有效诱导精子顶体反应的浓度的糖胺聚糖。 然后通过光学显微镜观察来计数来自每个部分的培养的精子的代表性样品,以测量与对照部分相比测试部分顶体反应的增加。 提供了一个套件,方便执行该方法。 该试剂盒包括无菌液体培养基,无菌可封闭的培养容器,以及以已知量计量的基本无菌的选定的无菌GAG的供应物,作为用作测试容器的每个培养容器。