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公开(公告)号：US20240254424A1
公开(公告)日：2024-08-01
申请号：US18614957
申请日：2024-03-25
申请人： Jian Chen
发明人： Jian Chen
CPC分类号： C12M35/02 , B01L3/508 , C12M1/24 , C12M1/42 , C12M23/06 , C12M23/08 , B01L2300/027 , B01L2300/0663
摘要： A method for electroporation of biological cells, comprising the steps of: providing a shock tube for cell electroporation; filling up the cavity with the cell sample comprising cells and materials to be injected into the cells, wherein a bulged liquid surface is formed; securing the stopple to a second end of the tube by a positioning structure, while generating a compressive deformation to the elastic piece between the stopple and the second electrode; connecting the first electrode and the second electrode with a pulse power supply to perform electroporation.
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2.发明公开公开(公告)号：US20230383222A1
公开(公告)日：2023-11-30
申请号：US18061481
申请日：2022-12-05
申请人： DAAN GENE CO., LTD.
发明人： XIWEN JIANG , LINBO LIU , HAITAO SHU , JUNJIE LI
CPC分类号： C12M1/24 , C12Q1/68 , B01L3/50273 , B01L2200/10 , B01L2200/0689
摘要： Disclosed are a hand-held nucleic acid detection apparatus equipped with microfluidic chip, and a use method thereof. The detection apparatus includes a sample collection tube, a chip connector, and a detection chip. The chip connector is provided with a piercing tube configured to pierce the sample collection tube. The detection chip is connected to the chip connector. The detection chip is provided with a plurality of chambers and a plurality of flow channels. The flow channels are in communication with the piercing tube and the chambers. The reaction reagent and all the reaction processes are integrated on a chip, with no need of solution injection by virtue of an external instrument, solution transfer or the like operations, and the nucleic acid detection may be carried out even in the case of no dedicated experiment sites or special conditions.
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3.发明申请公开(公告)号：US20180251713A1
公开(公告)日：2018-09-06
申请号：US15909523
申请日：2018-03-01
申请人： FLUIDION SAS
发明人： Dan E. Angelescu , Andreas Hausot
CPC分类号： C12M1/34 , C12M1/08 , C12M1/24 , C12M1/26 , C12M1/38 , C12M23/08 , C12M33/06 , C12M33/07 , C12M33/12 , G01N1/28 , G01N21/3151 , G01N21/645 , G01N21/6486 , G01N21/83
摘要： A system for processing samples from a body of fluid. The system includes one or more sample bottles for acquiring a sample from the body of fluid. Each sample bottle initially retains a pre-filling fluid. Each sample bottle includes a fluidic inlet port and a bottle outlet port. Each sample bottle has an inlet check valve coupled to the fluidic inlet port, the inlet check valve configured to allow fluid from the body of fluid into a sample bottle via the fluidic inlet port when the pressure difference between the body of fluid and fluid within the sample bottle reaches a threshold. The system further includes at least one pump, the bottle outlet port of each sample bottle selectively coupled to the at least one pump via a different control valve. The at least one pump is configured, in a first configuration, to remove prefilling fluid from each selected sample bottle such that, for each selected sample bottle, the pressure difference threshold is reached and a sample from the body of fluid is acquired.
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公开(公告)号：US11965149B2
公开(公告)日：2024-04-23
申请号：US16586989
申请日：2019-09-29
申请人： Jian Chen
发明人： Jian Chen
CPC分类号： C12M35/02 , B01L3/508 , C12M1/24 , C12M1/42 , C12M23/06 , C12M23/08 , B01L2300/027 , B01L2300/0663
摘要： The shock tube comprises a tube, a first electrode, a second electrode and a stopple, wherein the tube is internally provided with a cavity for accommodating a target liquid sample. The first electrode is arranged at one end of the tube. The second electrode is arranged in the stopple, and the outer end of the second electrode can be electrically connected with the exterior via an opening of the stopple. The stopple is internally provided with an elastic piece connected with the second electrode. The outer side of the elastic piece is connected with the stopple, and the inner side of the elastic piece is connected with the second electrode. The invention further provides a cell electroporation device where the shock tube can be placed.
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5.发明公开公开(公告)号：US20230227759A1
公开(公告)日：2023-07-20
申请号：US18189059
申请日：2023-03-23
申请人： FLUIDION SAS
发明人： Dan E. Angelescu , Andreas Hausot
IPC分类号： C12M1/34 , G01N21/64 , G01N21/83 , C12M1/08 , C12M1/24 , C12M1/32 , C12M1/38 , C12M1/26 , G01N1/28 , G01N21/31
CPC分类号： C12M1/34 , G01N21/6486 , G01N21/83 , C12M1/08 , C12M1/24 , C12M33/06 , C12M1/38 , C12M23/08 , C12M33/07 , C12M33/12 , C12M1/26 , G01N1/28 , G01N21/645 , G01N21/3151
摘要： A system for processing samples from a body of fluid. The system includes one or more sample bottles for acquiring a sample from the body of fluid. Each sample bottle initially retains a pre-filling fluid. Each sample bottle includes a fluidic inlet port and a bottle outlet port. Each sample bottle has an inlet check valve coupled to the fluidic inlet port, the inlet check valve configured to allow fluid from the body of fluid into a sample bottle via the fluidic inlet port when the pressure difference between the body of fluid and fluid within the sample bottle reaches a threshold. The system further includes at least one pump, the bottle outlet port of each sample bottle selectively coupled to the at least one pump via a different control valve. The at least one pump is configured, in a first configuration, to remove prefilling fluid from each selected sample bottle such that, for each selected sample bottle, the pressure difference threshold is reached and a sample from the body of fluid is acquired.
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6.发明申请公开(公告)号：US20180332818A1
公开(公告)日：2018-11-22
申请号：US15985558
申请日：2018-05-21
发明人： Chia-Kang TSAO
CPC分类号： A01K1/03 , A01K29/00 , A01K67/033 , A01K2227/706 , A01K2267/03 , B65G47/90 , B65G2201/0244 , C12M1/24
摘要： A culture container includes a tube having a first and a second opening respectively provided at two opposite ends thereof, and a cover installable on and removable from the first opening of the tube, the cover including a receptacle for holding a substance consumable by an organism of interest, the receptacle being enclosed inside the tube when the cover is installed on the first opening of the tube. Moreover, a system and a method of transferring a cultured organism of interest include switching the covers between two culture containers.
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公开(公告)号：US20140023813A1
公开(公告)日：2014-01-23
申请号：US14110117
申请日：2012-04-17
IPC分类号： B41J2/44
CPC分类号： A61D19/024 , A01N1/0268 , B23K26/18 , B23K26/355 , B23K26/361 , B23K26/362 , B23K26/40 , B23K2101/007 , B23K2103/42 , B29C59/165 , B41J2/442 , B41M5/267 , C12M1/24 , G03C1/733 , G03C5/56 , Y10T428/139
摘要： Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.
摘要翻译： 通常，聚合物构件和用于在聚合物构件上产生可见痕迹的激光标记方法，例如在薄和/或弯曲表面上。 激光标记方法可以包括激光标记吸管的方法，其步骤是将激光源性质与标记的吸管的性质或激光标记具有光致变色染料的吸管的步骤相匹配。
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公开(公告)号：US20120264207A1
公开(公告)日：2012-10-18
申请号：US13448948
申请日：2012-04-17
CPC分类号： A61D19/024 , A01N1/0268 , B23K26/18 , B23K26/355 , B23K26/361 , B23K26/362 , B23K26/40 , B23K2101/007 , B23K2103/42 , B29C59/165 , B41J2/442 , B41M5/267 , C12M1/24 , G03C1/733 , G03C5/56 , Y10T428/139
摘要： Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.
摘要翻译： 通常，聚合物构件和用于在聚合物构件上产生可见痕迹的激光标记方法，例如在薄和/或弯曲表面上。 激光标记方法可以包括激光标记吸管的方法，其步骤是将激光源性质与标记的吸管的性质或激光标记具有光致变色染料的吸管的步骤相匹配。
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公开(公告)号：US11930810B2
公开(公告)日：2024-03-19
申请号：US16332709
申请日：2017-09-12
申请人： 77 KC CO., LTD.
发明人： Hirohide Komatsu , Tsutomu Sawa
IPC分类号： A01N1/02 , A61B17/43 , A61B17/435 , A61D19/02 , A61J3/00 , C12M1/24 , G06K19/077
CPC分类号： A01N1/0268 , A01N1/02 , A61B17/43 , A61B17/435 , A61D19/02 , A61D19/024 , A61J3/00 , C12M1/24 , G06K19/077 , G06K19/07758
摘要： Provided is a cryopreservation container that prevents confusion of specimens to be used for treatment and that is suitable for individual management of fertilized eggs and the like to be cryopreserved. IC tags are attached to a cryopreservation tank 61, a canister 51, a cane 41, and cryopreservation containers such as an ovum storage container 1 and sperm storage containers 71, 81. The cryopreservation containers may also have individual identification codes attached hereto. An ovum storage container 11 is configured by fitting an IC tag 12 from the rear end of a rod-like part 2 of a conventional ovum storage container 1. In addition, the IC tag 12 is formed by providing an inlet 15 inside a thin-walled pipe 14 made of a synthetic resin. The inlet 15 may be fixed at both ends by means of fixing members 18, 18 composed of a nonconductive material, or may be integrally molded with a nonconductive synthetic resin material 20.
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公开(公告)号：US20230332092A1
公开(公告)日：2023-10-19
申请号：US18211267
申请日：2023-06-18
发明人： Howard Cannon , Thomas Forest Farb
摘要： A data collection hub and method wherein a controller is configured to receive data from a plurality of sensors sensing conditions related to the performance of operations on cells by at least one instrument. A database stores the data from the plurality of sensors and the controller is configured to compare data from the plurality of sensors for a past operation with the data for a more recent operation.
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