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    Cell culture vessel with self-maintained atmosphere
    1.
    发明授权
    Cell culture vessel with self-maintained atmosphere 失效
    细胞培养容器具有自我维持的气氛

    公开(公告)号：US5801054A

    公开(公告)日：1998-09-01

    申请号：US716691

    申请日：1996-09-19

    申请人： Johnathan L. Kiel ,  John L. Alls

    发明人： Johnathan L. Kiel ,  John L. Alls

    IPC分类号： C12M1/04 ,  C12M3/00 ,  C12M1/24

    CPC分类号： C12M23/08 ,  C12M41/34

    摘要： A cell culture device is described comprising a gas permeable vessel which contains one or more self contained carbon dioxide generators and which is attached to a closure closing a gas permeable cell culture flask. Located in the closure is a gas permeable insert which defines a gas permeable opening through which the gases from the atmosphere of the vessel produced by the carbon dioxide generators can communicate with the gases in the atmosphere of the flask. This invention allows the rapid and uniform equilibration of the atmosphere of the flask with the controlled atmosphere of the vessel while still providing a closed system which prevents the entry of microbial organisms into the flask. This invention provides a controlled atmosphere for up to 24 hours that maintains the pH of the culture medium for optimal growth of cells growing in the culture medium in the flask. This invention allows for continuous microscopic observation of cells during this period and for long term exposure of cells to various physical or chemical agents.

    摘要翻译： 描述了一种细胞培养装置，其包括含有一个或多个自含二氧化碳发生器的气体可渗透容器，并且附着于闭合气体可渗透细胞培养瓶的封闭件。 位于封闭件中的是可透气的插入件，其限定了可透气的开口，来自二氧化碳发生器产生的容器的气体的气体可以通过该气体与烧瓶大气中的气体连通。 本发明允许烧瓶的气氛与容器的受控气氛快速均匀地平衡，同时仍然提供一种封闭系统，其防止微生物进入烧瓶。 本发明提供可控制的气氛长达24小时，其维持培养基的pH以使培养基中培养基中生长的细胞生长最佳。 本发明允许在此期间连续显微镜观察细胞，并允许细胞长期暴露于各种物理或化学试剂。

    Organic semiconductor recognition complex and system
    4.
    发明授权
    Organic semiconductor recognition complex and system 有权
    有机半导体识别复杂系统

    公开(公告)号：US06303316B1

    公开(公告)日：2001-10-16

    申请号：US09608706

    申请日：2000-06-30

    申请人： Johnathan L. Kiel ,  John G. Bruno ,  Jill E. Parker ,  John L. Alls ,  Charles R. Batishko ,  Eric A. Holwitt

    发明人： Johnathan L. Kiel ,  John G. Bruno ,  Jill E. Parker ,  John L. Alls ,  Charles R. Batishko ,  Eric A. Holwitt

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6825 ,  C12Q1/6837 ,  Y10T436/143333 ,  C12Q2565/607

    摘要： In a recognition complex system, nucleic acid ligands comprising random DNA sequences are operatively coupled to an organic semiconductor and distributed so as to form an array of recognition complexes. When an unknown chemical or biological analyte is applied to the array, the electrical and/or photochemical properties of one or more of the recognition complexes are altered upon binding of the nucleic acid ligand to the analyte. The degree to which the electrical and/or photochemical properties change is a function of the affinity of the nucleic acid ligand sequence for the analyte. The electrical and photochemical changes associated with the array, as a whole, can be used as a unique signature to identify the analyte. In certain embodiments, an iterative process of selection and amplification of nucleic acid ligands that bind to the analyte can be used to generate a new array with greater affinity and specificity for a target analyte, or to produce one or more nucleic acid ligands with high binding affinity for an analyte. The present invention also provides methods for preparing nucleic acid ligands that bind with high affinity to an analyte and using such nucleic acid ligands to neutralize the analyte.

    摘要翻译： 在识别复合体系中，包含随机DNA序列的核酸配体可操作地偶联到有机半导体上，并分布以形成识别复合物的阵列。 当将未知的化学或生物分析物施加到阵列时，一个或多个识别复合物的电和/或光化学性质在核酸配体与分析物结合时被改变。 电和/或光化学性质变化的程度是核酸配体序列对分析物的亲和力的函数。 与阵列相关联的电学和光化学变化作为一个整体，可以用作识别分析物的独特标记。 在某些实施方案中，结合分析物的核酸配体的选择和扩增的迭代过程可用于产生对靶分析物具有更大亲和性和特异性的新阵列，或产生具有高结合力的一个或多个核酸配体 对分析物的亲和力。 本发明还提供了制备以高亲和力结合分析物并使用这种核酸配体中和分析物的核酸配体的方法。

    Rapid identification of environmental bacillus
    5.
    发明授权
    Rapid identification of environmental bacillus 失效
    快速鉴定环境杆菌

    公开(公告)号：US5156971A

    公开(公告)日：1992-10-20

    申请号：US821525

    申请日：1992-01-15

    申请人： Johnathan L. Kiel ,  John L. Alls ,  Richard A. Weber ,  Jill E. Parker

    发明人： Johnathan L. Kiel ,  John L. Alls ,  Richard A. Weber ,  Jill E. Parker

    IPC分类号： C12N1/20 ,  C12Q1/04

    CPC分类号： C12R1/07 ,  C12N1/20 ,  C12Q1/04 ,  G01N2333/32

    摘要： A diagnostic test for environmental bacillus which comprises the steps of inoculating an agar growth medium comprising a nitrate source, luminol and 3-amino-L-tyrosine (3AT) with the sample, incubating the inoculated medium and determining the presence of the bacillus. The novel medium preferably comprises potassium nitrate, luminol, 3-amino-L-tyrosine and trypticase soy agar. Antibiotics and/or a specific bacteriophage may be added to the medium surface in localized areas to show specific bacterial lysis for identification. The novel medium and the methods of this invention are suitable for the identification of B. anthracis.

    摘要翻译： 环境芽孢杆菌的诊断试验包括以下步骤：将含有硝酸盐源，鲁米诺和3-氨基-L-酪氨酸（3AT）的琼脂生长培养基与样品接种，培养接种的培养基并确定芽孢杆菌的存在。 该新型培养基优选包含硝酸钾，鲁米诺，3-氨基-L-酪氨酸和胰蛋白酶大豆琼脂。 可将抗生素和/或特异性噬菌体加入局部区域的培养基表面，以显示特异性细菌裂解进行鉴定。 本发明的新型培养基和方法适用于鉴定炭疽杆菌。