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1.发明授权Process for producing the enzyme D-amino acid oxidase of Rhodotorula gracilis in host cells 失效在宿主细胞中生产细小红球菌的D-氨基酸氧化酶的方法公开(公告)号：US06187574B1
公开(公告)日：2001-02-13
申请号：US08973914
申请日：1998-04-28
申请人： Jose Luis Garcia Lopez , Estrella Cortes Rubio , Jorge Alonso Palacios , Jose Luis Barredo Fuente , Bruno Diez Garcia , Miguel Angel Moreno Valle , Carmen Schleissner Sanchez , Alfonso Collados de la Vieja , Alejandro Vitaller Alba , Francisco Salto Maldonado
发明人： Jose Luis Garcia Lopez , Estrella Cortes Rubio , Jorge Alonso Palacios , Jose Luis Barredo Fuente , Bruno Diez Garcia , Miguel Angel Moreno Valle , Carmen Schleissner Sanchez , Alfonso Collados de la Vieja , Alejandro Vitaller Alba , Francisco Salto Maldonado
IPC分类号： C12N902
CPC分类号： C12N9/0024
摘要： Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.
摘要翻译： 在宿主细胞中生产细小红球菌的D-氨基酸氧化酶酶的方法。 表达酶的过程包括分离与编码产生所述酶的任何红曲霉菌株的D-氨基酸氧化酶的基因的信使RNA相对应的互补DNA; 将编码红景天细菌的D-氨基酸氧化酶的DNA片段与包含与核糖体结合的位点的DNA序列和用于在宿主细胞中表达基因的高效启动子序列融合; 将DNA片段插入适合宿主细胞的质粒中; 培养用所述质粒转化的宿主细胞; 并收集酶。
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2.发明授权Process for increasing the production of penicillin G (benzylpenicillin) in Penicillium chrysogenum by expression of the PCL gene 失效通过PCL基因表达增加产黄青霉菌青霉素G（苄青霉素）的生产方法公开(公告)号：US06251655B1
公开(公告)日：2001-06-26
申请号：US08952457
申请日：1998-10-28
申请人： Baltasar Minambres Rodriguez , Honorina Martinez Blanco , Elias Rodriguez Olivera , Belen Garcia Alonso , Jose Manuel Fernandez Canon , Jose Luis Barredo Fuente , Bruno Diez Garcia , Carmen Schleissner Sanchez , Miguel Angel Moreno Valle , Francisco Salto Maldonado , Jose Maria Luengo Rodriguez
发明人： Baltasar Minambres Rodriguez , Honorina Martinez Blanco , Elias Rodriguez Olivera , Belen Garcia Alonso , Jose Manuel Fernandez Canon , Jose Luis Barredo Fuente , Bruno Diez Garcia , Carmen Schleissner Sanchez , Miguel Angel Moreno Valle , Francisco Salto Maldonado , Jose Maria Luengo Rodriguez
IPC分类号： C12N120
CPC分类号： C12N9/93 , C12N2310/111 , C12P37/02
摘要： An isolated DNA encoding phenyl acetyl-CoA-ligase and a process of increasing the production of penicillin G in a strain of Penicillium chrysogenum by transforming the strain with the isolated DNA. Also vectors and host organisms having the isolated DNA.
摘要翻译： 编码苯乙酰辅酶A连接酶的分离DNA和通过用分离的DNA转化菌株来增加产黄青霉菌株中青霉素G的产生的方法。 还有具有分离的DNA的载体和宿主生物体。
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3.发明申请Method of production of astaxanthin by fermenting selected strains of xanthophyllomyces dendrorhous 审中-公开通过发酵选择的黄色叶黄素树突状菌株生产虾青素的方法公开(公告)号：US20050124032A1
公开(公告)日：2005-06-09
申请号：US10501364
申请日：2003-02-03
申请人： Juan De La Fuente Moreno , Enrique Peiro Cezon , Bruno Diez Garcia , Ana Marcos Rodriguez , Carmen Schleissner Sanchez , Marta Rodriguez Saiz , Carmelita Rodriguez Otero , Walter Cabri , Jose Barredo Fuente
发明人： Juan De La Fuente Moreno , Enrique Peiro Cezon , Bruno Diez Garcia , Ana Marcos Rodriguez , Carmen Schleissner Sanchez , Marta Rodriguez Saiz , Carmelita Rodriguez Otero , Walter Cabri , Jose Barredo Fuente
IPC分类号： C12N1/21 , A23K1/00 , A23K1/16 , A23K1/18 , A23L1/28 , A23L1/30 , C12N1/14 , C12N15/01 , C12P23/00 , C12R1/645 , C12N15/00
CPC分类号： C12P23/00 , A23K10/12 , A23K20/174 , A23K20/179 , A23K50/80 , A23L31/00 , A23V2002/00 , A23V2250/208
摘要： The present invention describes methods for (i) obtaining astaxanthin-overproducing strains of X. dendrorhous and (ii) the use of the aforementioned strains in improved conditions of fermentation. The methods used for selecting the strains are based on: (i) resistance to inhibitors of steroid synthesis, to inhibitors of respiration and to compounds that induce the formation of free radicals, (ii) color intensity of the colony and production of carotenoids on solid medium, (iii) production of astaxanthin in darkness, (iv) production of astaxanthin in conditions with a raised temperature and (v) production of astaxanthin with carbon sources other than glucose. These methods permit the efficient selection of mutants of X. dendrorhous with astaxanthin yields higher than the parent strain and little accumulation of other carotenoids. Cultivation of X. dendrorhous in liquid medium makes it possible to obtain a biomass that can be used directly in human and animal nutrition.
摘要翻译： 本发明描述了（i）获得十二指肠杆菌的过量产生虾青素的方法和（ii）上述菌株在改良的发酵条件下的用途。 用于选择菌株的方法基于：（i）对类固醇合成抑制剂，呼吸抑制剂和诱导自由基形成的化合物的抗性，（ii）集落的色彩强度和固体上类胡萝卜素的产生 培养基，（iii）在黑暗中生产虾青素，（iv）在温度升高的条件下生成虾青素，和（v）生产除葡萄糖以外的碳源的虾青素。 这些方法允许有效选择X. dendrorhous的突变体，虾青素的产量高于亲本菌株，并且其他类胡萝卜素的积累很少。 在液体培养基中培养X.树枝状结构使得可以获得可以直接用于人和动物营养的生物质。
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4.发明申请Gene cluster involved in safracin biosynthesis and its uses for genetic engineering 失效涉及safracin生物合成的基因簇及其在遗传工程中的应用公开(公告)号：US20060134764A1
公开(公告)日：2006-06-22
申请号：US10540092
申请日：2003-12-19
申请人： Ana Velasco Iglesias , Fernando De La Calle , Tomas Aparicio Perez , Carmen Schleissner Sanchez , Paloma Acebo Pais , Pilar Rodriguez Ramos , Fernando Reyes Benitez , Ruben Henriquez Pelaez
发明人： Ana Velasco Iglesias , Fernando De La Calle , Tomas Aparicio Perez , Carmen Schleissner Sanchez , Paloma Acebo Pais , Pilar Rodriguez Ramos , Fernando Reyes Benitez , Ruben Henriquez Pelaez
IPC分类号： C12P17/18 , A61K31/498 , C12N1/21 , C07D487/14
CPC分类号： C12N9/0004 , A61K31/4995 , C07D401/12 , C07D403/12 , C07D413/14 , C07D471/04 , C07D471/14 , C07K14/21 , C12N9/00 , C12N9/10 , C12N15/52 , C12P17/12 , C12P17/182
摘要： A gene cluster has open reading frames which encode polypeptides sufficient to direct the synthesis of a safracin molecule.
摘要翻译： 基因簇具有开放阅读框，其编码足以引导safracin分子合成的多肽。
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5.发明申请Genes Involved in the Biosynthesis of Thiocoraline and Heterologous Production of Same 审中-公开涉及Thorcoraline的生物合成和异源生产的基因公开(公告)号：US20090130675A1
公开(公告)日：2009-05-21
申请号：US11997692
申请日：2006-08-01
申请人： Angelina Ramos Castro , Alfredo Fernandez Brana , Felipe Lombo Brugos , Carmen Mendez Fernandez , Jose Antonio Salas Fernandez , Ana Velasco Iglesias , Carmen Schleissner Sanchez
发明人： Angelina Ramos Castro , Alfredo Fernandez Brana , Felipe Lombo Brugos , Carmen Mendez Fernandez , Jose Antonio Salas Fernandez , Ana Velasco Iglesias , Carmen Schleissner Sanchez
CPC分类号： C12N15/52 , C12P17/187
摘要： The invention relates to genes involved in the biosynthesis of thiocoraline and to the heterologous production of same. According to the invention, the cluster of genes responsible for the biosynthesis of thiocoraline was identified and cloned. Said cluster of genes can be used in the heterologous production of thiocoraline which has an antitumor and antibacterial activity.
摘要翻译： 本发明涉及参与硫代卡拉品的生物合成及其异源生产的基因。 根据本发明，鉴定并克隆了负责硫代卡罗林生物合成的基因簇。 所述基因簇可用于具有抗肿瘤和抗菌活性的硫代西洛他异源生产。
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