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    Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3′ flap
    4.
    发明授权
    Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3′ flap 有权
    使用具有3'瓣的探针检测靶核酸序列的方法和组合物

    公开(公告)号：US07625705B2

    公开(公告)日：2009-12-01

    申请号：US11701605

    申请日：2007-02-01

    申请人： Andrew Firmin ,  Joseph A. Sorge

    发明人： Andrew Firmin ,  Joseph A. Sorge

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6823 ,  C12Q2525/161 ,  C12Q2521/319

    摘要： The invention provides compositions, kits and methods of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure. The cleavage structure is formed by incubating a sample containing a target nucleic acid with a downstream probe that forms a 3′ flap when hybridized to the target. The cleavage structure is cleaved with a 3′ nuclease and a detectable signal is produced.

    摘要翻译： 本发明提供通过形成切割结构产生指示样品中目标核酸序列存在的信号的组合物，试剂盒和方法。 通过将含有靶核酸的样品与与靶标杂交时形成3'瓣的下游探针孵育来形成切割结构。 切割结构用3'核酸酶切割并产生可检测的信号。

    Key probe compositions and methods for polynucleotide detection
    5.
    发明授权
    Key probe compositions and methods for polynucleotide detection 失效
    用于多核苷酸检测的关键探针组合物和方法

    公开(公告)号：US07504218B2

    公开(公告)日：2009-03-17

    申请号：US11351129

    申请日：2006-02-09

    申请人： Scott B Happe ,  Joseph A Sorge ,  Andrew Firmin

    发明人： Scott B Happe ,  Joseph A Sorge ,  Andrew Firmin

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q2525/301

    摘要： The invention relates to compositions and methods for detection of nucleic acid sequences. The invention further relates to kit format of said compositions for detection of nucleic acid sequences. Oligonucleotide probes of the invention comprise a target binding sequence and a sequence at least partially complementary thereto, joined by an optional linker to form a hairpin structure in the absence of the target nucleic acid sequence. The probes of the invention comprise a pair of moieties that produce a detectable signal when the probe hybridizes to the target sequence.

    摘要翻译： 本发明涉及用于检测核酸序列的组合物和方法。 本发明还涉及用于检测核酸序列的所述组合物的试剂盒形式。 本发明的寡核苷酸探针包含靶结合序列和与其至少部分互补的序列，通过任选的接头连接以在不存在靶核酸序列的情况下形成发夹结构。 本发明的探针包含当探针与靶序列杂交时产生可检测信号的一对部分。

    Key probe compositions and methods for polynucleotide detection
    7.
    发明申请
    Key probe compositions and methods for polynucleotide detection 失效

    公开(公告)号：US20060292592A1

    公开(公告)日：2006-12-28

    申请号：US11351129

    申请日：2006-02-09

    申请人： Scott Happe ,  Joseph Sorge ,  Andrew Firmin

    发明人： Scott Happe ,  Joseph Sorge ,  Andrew Firmin

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q2525/301

    摘要： The invention relates to compositions and methods for detection of nucleic acid sequences. The invention further relates to kit format of said compositions for detection of nucleic acid sequences. Oligonucleotide probes of the invention comprise a target binding sequence and a sequence at least partially complementary thereto, joined by an optional linker to form a hairpin structure in the absence of the target nucleic acid sequence. The probes of the invention comprise a pair of moieties that produce a detectable signal when the probe hybridizes to the target sequence.

    Triplex probe compositions and methods for polynucleotide detection
    8.
    发明申请
    Triplex probe compositions and methods for polynucleotide detection 审中-公开
    三重探针组合物和多核苷酸检测方法

    公开(公告)号：US20060194222A1

    公开(公告)日：2006-08-31

    申请号：US11254334

    申请日：2005-10-20

    申请人： Joseph Sorge ,  Scott Happe ,  Andrew Firmin

    发明人： Joseph Sorge ,  Scott Happe ,  Andrew Firmin

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q1/6839 ,  C12Q2565/101 ,  C12Q2537/119

    摘要： The present invention provides composition and methods for the detection and measurement of target nucleic acids. The probes of the present invention, or triplex probes, comprise a complex of three oligonucleotide probes including: (1) a first oligonucleotide probe, (2) a second oligonucleotide probe, and (3) a bridging oligonucleotide probe. In most aspects of the invention, the first and second oligonucleotide probes preferentially hybridize to the bridging oligonucleotide in the absence of a target nucleic acid. The first oligonucleotide probe contains one member of an interactive pair of labels and the second oligonucleotide probe contains the other member of the interactive pair of labels. Separation of the first and second oligonucleotide probes (e.g., binding to target, cleavage of first, second, or bridging oligonucleotide) generates a detectable signal indicating the presence of a target nucleic acid.

    摘要翻译： 本发明提供了用于检测和测量靶核酸的组合物和方法。 本发明的探针或三重探针包含三种寡核苷酸探针的复合物，其包括：（1）第一寡核苷酸探针，（2）第二寡核苷酸探针和（3）桥连寡核苷酸探针。 在本发明的大多数方面，第一和第二寡核苷酸探针在不存在靶核酸的情况下优先与桥连寡核苷酸杂交。 第一寡核苷酸探针包含交互对标签的一个成员，第二寡核苷酸探针包含交互式对标签的另一成员。 分离第一和第二寡核苷酸探针（例如，与靶标结合，第一，第二或桥接寡核苷酸的切割）产生指示存在靶核酸的可检测信号。

    Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap
    9.
    发明申请
    Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap 有权
    使用具有3'瓣的探针检测靶核酸序列的方法和组合物

    公开(公告)号：US20080026387A1

    公开(公告)日：2008-01-31

    申请号：US11701605

    申请日：2007-02-01

    申请人： Andrew Firmin ,  Joseph Sorge

    发明人： Andrew Firmin ,  Joseph Sorge

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N9/12

    CPC分类号： C12Q1/6823 ,  C12Q2525/161 ,  C12Q2521/319

    摘要： The invention provides compositions, kits and methods of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure. The cleavage structure is formed by incubating a sample containing a target nucleic acid with a downstream probe that forms a 3′ flap when hybridized to the target. The cleavage structure is cleaved with a 3′ nuclease and a detectable signal is produced.

    摘要翻译： 本发明提供通过形成切割结构产生指示样品中目标核酸序列存在的信号的组合物，试剂盒和方法。 通过将含有靶核酸的样品与与靶标杂交时形成3'瓣的下游探针孵育来形成切割结构。 切割结构用3'核酸酶切割并产生可检测的信号。