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    Methods of enriching for and identifying polymorphisms
    1.
    发明授权
    Methods of enriching for and identifying polymorphisms 有权
    富集和鉴定多态性的方法

    公开(公告)号：US08383343B2

    公开(公告)日：2013-02-26

    申请号：US10391090

    申请日：2003-03-18

    申请人： Joseph A. Sorge

    发明人： Joseph A. Sorge

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2565/519 ,  C12Q2565/531 ,  C12Q2533/101 ,  C12Q2522/101

    摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.

    摘要翻译： 本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内 复杂性，然后鉴定富集的核酸样品内的多态性。 公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法， 允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性 分子。

    METHODS OF ENRICHING FOR AND IDENTIFYING POLYMORPHISMS
    3.
    发明申请
    METHODS OF ENRICHING FOR AND IDENTIFYING POLYMORPHISMS 审中-公开
    增强和鉴别多态性的方法

    公开(公告)号：US20100248246A1

    公开(公告)日：2010-09-30

    申请号：US12749070

    申请日：2010-03-29

    申请人： Joseph A. Sorge ,  Ronald W. Davis

    发明人： Joseph A. Sorge ,  Ronald W. Davis

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2565/519 ,  C12Q2565/531 ,  C12Q2533/101 ,  C12Q2522/101

    摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.

    摘要翻译： 本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内 复杂性，然后鉴定富集的核酸样品内的多态性。 公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法， 允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性 分子。

    Compositions and methods utilizing DNA polymerases
    5.
    发明申请
    Compositions and methods utilizing DNA polymerases 审中-公开
    使用DNA聚合酶的组合物和方法

    公开(公告)号：US20080199935A1

    公开(公告)日：2008-08-21

    申请号：US11644729

    申请日：2006-12-22

    申请人： Joseph A. Sorge ,  Holly Hogrefe ,  Connie J. Hansen

    发明人： Joseph A. Sorge ,  Holly Hogrefe ,  Connie J. Hansen

    IPC分类号： C12N9/12

    CPC分类号： B82Y5/00 ,  B82Y10/00 ,  C12N9/1252

    摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.

    摘要翻译： 本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。 本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

    Methods for detection of a target nucleic acid by capture
    6.
    发明授权
    Methods for detection of a target nucleic acid by capture 有权
    通过捕获检测靶核酸的方法

    公开(公告)号：US07361467B2

    公开(公告)日：2008-04-22

    申请号：US11150775

    申请日：2005-06-10

    申请人： Joseph A. Sorge ,  Anne M. Whalen

    发明人： Joseph A. Sorge ,  Anne M. Whalen

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6823 ,  C12Q1/6816 ,  C12Q2521/301 ,  C12Q2531/113 ,  C12Q2521/307 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2561/109 ,  C12Q2565/519 ,  C12Q2561/101 ,  C12Q2521/107

    摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.The invention also relates to a method of detecting or measuring a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to a target nucleic acid and comprising a binding moiety, and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.

    摘要翻译： 本发明涉及产生指示样品中目标核酸存在的信号的方法，其中该方法包括通过将包含靶核酸的样品与具有二级结构变化的探针一起孵育形成切割结构 探针与靶核酸的结合并且还包含结合部分。 本发明还包括用核酸酶切割切割结构以释放核酸片段以产生信号的步骤，其中产生信号指示样品中靶核酸的存在，以及检测和/或测量 通过结合部分与固体支持物上的捕获元件结合捕获的片段的量。 本发明还涉及一种检测或测量样品中的靶核酸的方法，其中该方法包括通过将含有靶核酸的样品与具有在探针结合后变化的二级结构的探针形成切割结构 并且包含结合部分，并用核酸酶切割切割结构以产生切割的核酸片段，并通过结合部分与捕获元件的结合捕获的片段的量，并检测和/或测量 坚实的支持。

    Methods for detection of a nucleic acid by sequential amplification
    8.
    发明授权
    Methods for detection of a nucleic acid by sequential amplification 有权
    通过连续扩增检测核酸的方法

    公开(公告)号：US06893819B1

    公开(公告)日：2005-05-17

    申请号：US09717602

    申请日：2000-11-21

    申请人： Joseph A. Sorge

    发明人： Joseph A. Sorge

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6844 ,  C12Q1/6823 ,  C12Q2537/149 ,  C12Q2531/101 ,  C12Q2525/161

    摘要： The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification. The method of the invention includes the steps of forming at least a first cleavage structure comprising at least one flap, cleaving the cleavage structure with a cleavage means to release the at least one flap and detecting the at least one flap. In one embodiment of the invention, the method includes the additional steps of forming at least a second cleavage structure comprising the at least one released flap from the first cleavage structure cleaving the second cleavage structure with a cleavage means to release at least one flap from the second cleavage structure and detecting released flaps from the first or second cleavage structure.

    摘要翻译： 本发明涉及通过线性扩增检测靶核酸的方法。 本发明还涉及通过扩增检测靶核酸的方法。 本发明的方法包括以下步骤：至少形成包含至少一个瓣的第一切割结构，用裂解装置切割切割结构以释放至少一个瓣并检测至少一个瓣。 在本发明的一个实施方案中，所述方法包括形成至少第二切割结构的附加步骤，所述至少第二切割结构包含来自第一切割结构的至少一个释放的翼片，其利用切割装置切割第二切割结构，以将至少一个翼片从 第二裂解结构和从第一或第二裂解结构检​​测释放的瓣。

    Methods and adaptors for generating specific nucleic acid populations
    10.
    发明授权
    Methods and adaptors for generating specific nucleic acid populations 失效

    公开(公告)号：US6060245A

    公开(公告)日：2000-05-09

    申请号：US938835

    申请日：1997-09-26

    申请人： Joseph A. Sorge ,  Rebecca L. Mullinax

    发明人： Joseph A. Sorge ,  Rebecca L. Mullinax

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6855 ,  C12Q1/6809 ,  C12Q1/6811

    摘要： The present invention relates to methods and kits for generating or analyzing nucleic acid populations or desired nucleic acid sequences based upon replication or amplification reactions. The invention comprises methods employing adaptors ligated to nucleic acids that preferentially permit replication or amplification of desired nucleic acid sequences or preferentially eliminate undesired nucleic acids from replication or amplification. The invention also comprises adaptors useful in the methods and in kits for replicating or amplifying nucleic acids. In one embodiment, the adaptors function to protect desired nucleic acids from cleavage by a restriction enzyme while other nucleic acids are cleaved. The protected, desired nucleic acids can then be preferentially replicated or amplified. Accordingly, the invention can be used for the amplification of desired nucleic acids and the effective removal of undesired nucleic acids from a population.