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11 条记录 (耗时 0.14 秒)

    Key probe compositions and methods for polynucleotide detection
    5.
    发明申请
    Key probe compositions and methods for polynucleotide detection 失效

    公开(公告)号：US20060292592A1

    公开(公告)日：2006-12-28

    申请号：US11351129

    申请日：2006-02-09

    申请人： Scott Happe ,  Joseph Sorge ,  Andrew Firmin

    发明人： Scott Happe ,  Joseph Sorge ,  Andrew Firmin

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q2525/301

    摘要： The invention relates to compositions and methods for detection of nucleic acid sequences. The invention further relates to kit format of said compositions for detection of nucleic acid sequences. Oligonucleotide probes of the invention comprise a target binding sequence and a sequence at least partially complementary thereto, joined by an optional linker to form a hairpin structure in the absence of the target nucleic acid sequence. The probes of the invention comprise a pair of moieties that produce a detectable signal when the probe hybridizes to the target sequence.

    Triplex probe compositions and methods for polynucleotide detection
    6.
    发明申请
    Triplex probe compositions and methods for polynucleotide detection 审中-公开
    三重探针组合物和多核苷酸检测方法

    公开(公告)号：US20060194222A1

    公开(公告)日：2006-08-31

    申请号：US11254334

    申请日：2005-10-20

    申请人： Joseph Sorge ,  Scott Happe ,  Andrew Firmin

    发明人： Joseph Sorge ,  Scott Happe ,  Andrew Firmin

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q1/6839 ,  C12Q2565/101 ,  C12Q2537/119

    摘要： The present invention provides composition and methods for the detection and measurement of target nucleic acids. The probes of the present invention, or triplex probes, comprise a complex of three oligonucleotide probes including: (1) a first oligonucleotide probe, (2) a second oligonucleotide probe, and (3) a bridging oligonucleotide probe. In most aspects of the invention, the first and second oligonucleotide probes preferentially hybridize to the bridging oligonucleotide in the absence of a target nucleic acid. The first oligonucleotide probe contains one member of an interactive pair of labels and the second oligonucleotide probe contains the other member of the interactive pair of labels. Separation of the first and second oligonucleotide probes (e.g., binding to target, cleavage of first, second, or bridging oligonucleotide) generates a detectable signal indicating the presence of a target nucleic acid.

    摘要翻译： 本发明提供了用于检测和测量靶核酸的组合物和方法。 本发明的探针或三重探针包含三种寡核苷酸探针的复合物，其包括：（1）第一寡核苷酸探针，（2）第二寡核苷酸探针和（3）桥连寡核苷酸探针。 在本发明的大多数方面，第一和第二寡核苷酸探针在不存在靶核酸的情况下优先与桥连寡核苷酸杂交。 第一寡核苷酸探针包含交互对标签的一个成员，第二寡核苷酸探针包含交互式对标签的另一成员。 分离第一和第二寡核苷酸探针（例如，与靶标结合，第一，第二或桥接寡核苷酸的切割）产生指示存在靶核酸的可检测信号。

    Compositions and methods for the diagnosis of group B streptococcus infection
    7.
    发明申请
    Compositions and methods for the diagnosis of group B streptococcus infection 审中-公开
    B组链球菌感染的组成和方法

    公开(公告)号：US20060088849A1

    公开(公告)日：2006-04-27

    申请号：US11139257

    申请日：2005-05-27

    申请人： Scott Happe

    发明人： Scott Happe

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689

    摘要： The present invention relates to methods of detecting a Group B Streptococcus (GBS) bacterium in a sample. In particular, the present invention provides compositions, kits and methods for detecting the gbs1539 gene of a GBS bacterium.

    摘要翻译： 本发明涉及检测样品中B族链球菌（GBS）细菌的方法。 特别地，本发明提供了用于检测GBS细菌的gbs1539基因的组合物，试剂盒和方法。

    Methods and compositions for the detection of bacterial species
    9.
    发明申请
    Methods and compositions for the detection of bacterial species 审中-公开
    用于检测细菌种的方法和组合物

    公开(公告)号：US20050064451A1

    公开(公告)日：2005-03-24

    申请号：US10820971

    申请日：2004-04-08

    申请人： Scott Happe ,  Justin Brown ,  Frank Bozyan ,  Dwight Dubois

    发明人： Scott Happe ,  Justin Brown ,  Frank Bozyan ,  Dwight Dubois

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  G01N20060101

    CPC分类号： C12Q1/689 ,  C12Q2600/16

    摘要： The invention relates to compositions, methods and kits for the PCR-based detection of bacterial species. Methods are provided for increasing the specificity of a PCR-based bacterial assay. More specifically, primer sets and PCR-based assays are provided that amplify and detect conserved 16S rRNA gene sequences from multiple Mycoplasma species. The invention also provides kits for performing those assays, and compositions comprising oligonucleotide primers useful in those assays.

    摘要翻译： 本发明涉及用于细菌种类的基于PCR的检测的组合物，方法和试剂盒。 提供了用于增加基于PCR的细菌测定的特异性的方法。 更具体地，提供引物组和基于PCR的测定法，其扩增和检测来自多种支原体物种的保守的16S rRNA基因序列。 本发明还提供了用于进行这些测定的试剂盒，以及包含可用于那些测定中的寡核苷酸引物的组合物。

    Quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls
    10.
    发明授权
    Quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls 有权
    基于定量PCR的方法来预测使用重复DNA元件（线/正弦）作为阴性对照的下一代测序的靶富集效率

    公开(公告)号：US08663924B2

    公开(公告)日：2014-03-04

    申请号：US13308482

    申请日：2011-11-30

    申请人： Scott Happe ,  Joseph Hoang Hai Ong

    发明人： Scott Happe ,  Joseph Hoang Hai Ong

    IPC分类号： C12Q1/68 ,  C40B30/04 ,  C40B40/06 ,  C40B40/08

    CPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12Q2537/159 ,  C12Q2545/101

    摘要： A method for determining an efficiency of target enrichment from a DNA library, includes: adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and/or a positive control sequence from the library; determining a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence; performing enrichment of a target sequence from the DNA library using a bait sequence to produce a post-capture library; determining a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and determining the efficiency of the target enrichment, based on the post-capture amount of the positive control sequence, the post-capture amount of the negative control sequence, the pre-capture amount of the positive control sequence, and the pre-capture amount of the negative control sequence.

    摘要翻译： 用于确定DNA文库的靶富集效率的方法包括：向DNA文库添加阴性对照序列和阳性对照序列，或从文库中挑选阴性对照序列和/或阳性对照序列; 确定阴性对照序列的预捕获量和阳性对照序列的预捕获量; 使用诱饵序列从DNA文库中进行目标序列的富集以产生捕获后文库; 确定后捕获库中的阴性对照序列的后捕获量和阳性对照序列的后捕获量; 基于阳性对照序列的后捕获量，阴性对照序列的后捕获量，阳性对照序列的预捕获量和预捕获量，确定目标浓缩的效率 的阴性对照序列。