利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

22 条记录 (耗时 0.03 秒)

    Methods and vector constructs useful for production of recombinant AAV
    5.
    发明授权
    Methods and vector constructs useful for production of recombinant AAV 有权
    用于重组AAV生产的方法和载体构建体

    公开(公告)号：US06482634B1

    公开(公告)日：2002-11-19

    申请号：US09528470

    申请日：2000-03-17

    申请人： James M. Wilson ,  Weidong Xiao

    发明人： James M. Wilson ,  Weidong Xiao

    IPC分类号： C12N700

    CPC分类号： C12N15/86 ,  C12N2750/14143

    摘要： Methods for efficient production of recombinant AAV employ a host cell containing a first nucleic acid molecule comprising from 5′ to 3′, a parvovirus P5 promoter, a spacer, an AAV rep sequence and an AAV cap gene sequence, wherein said spacer is of sufficient size to reduce expression of the rep78 and rep68 gene products; a second nucleic acid molecule comprising a minigene comprising a transgene flanked by AAV inverse terminal repeats (ITRs) and under the control of regulatory sequences directing expression thereof in a host cell; and helper functions essential to the replication and packaging of rAAV, which functions are not provided by the first or second nucleic acid molecules. Host cells and molecule constructs are also described.

    摘要翻译： 用于有效生产重组AAV的方法使用含有包含5'至3'的第一核酸分子，细小病毒P5启动子，间隔子，AAV rep序列和AAV帽基因序列的宿主细胞，其中所述间隔基足够 大小以减少rep78和rep68基因产物的表达; 第二核酸分子包含包含侧翼为AAV逆末端重复（ITR）的转基因并在其引导其在宿主细胞中的表达的调节序列的控制下的小基因; 以及rAAV的复制和包装所必需的辅助功能，该功能不由第一或第二核酸分子提供。 还描述宿主细胞和分子构建体。

    COMPOSITIONS AND METHODS FOR THE PRODUCTION OF RECOMBINANT VIRUS VECTORS
    6.
    发明申请
    COMPOSITIONS AND METHODS FOR THE PRODUCTION OF RECOMBINANT VIRUS VECTORS 审中-公开
    用于生产重组病毒载体的组合物和方法

    公开(公告)号：US20130323817A1

    公开(公告)日：2013-12-05

    申请号：US13902280

    申请日：2013-05-24

    申请人： Weidong Xiao

    发明人： Weidong Xiao

    IPC分类号： C12N15/86

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10344 ,  C12N2710/10351 ,  C12N2710/16643 ,  C12N2710/16651 ,  C12N2710/24144 ,  C12N2750/14151 ,  C12N2750/14351 ,  C12N2760/20234

    摘要： A method for the production of a replication-deficient recombinant virus vector is disclosed. The replication-deficient recombinant virus vector has a recombinant virus genome with one or more defective viral genes. The method comprises infecting a host cell with a carrier virus having a carrier virus genome encoding one or more trans factors or variants thereof, incubating the infected host cell for a desired period of time, and isolating the replication-deficient recombinant virus vector. The carrier virus is a cytoplasmic virus that retains the carrier virus genome in the cytoplasm of the host cell. The host cell contains the recombinant viral genome and retains the recombinant viral genome in a nucleus of the host cell. Also disclosed is a carrier virus for the production of a replication-deficient recombinant virus vector.

    摘要翻译： 公开了一种制备复制缺陷型重组病毒载体的方法。 复制缺陷型重组病毒载体具有带有一个或多个缺陷病毒基因的重组病毒基因组。 该方法包括用载体病毒感染宿主细胞，所述载体病毒具有编码一种或多种反式因子或其变体的载体病毒基因组，将所感染的宿主细胞孵育所需时间，并分离复制缺陷型重组病毒载体。 载体病毒是将载体病毒基因组保留在宿主细胞的细胞质中的细胞质病毒。 宿主细胞含有重组病毒基因组，并将重组病毒基因组保留在宿主细胞的细胞核中。 还公开了用于产生复制缺陷型重组病毒载体的载体病毒。

    Methods and compositions for the production of recombinant virus vectors
    7.
    发明授权
    Methods and compositions for the production of recombinant virus vectors 有权
    用于生产重组病毒载体的方法和组合物

    公开(公告)号：US08263396B2

    公开(公告)日：2012-09-11

    申请号：US12662165

    申请日：2010-04-01

    申请人： Weidong Xiao

    发明人： Weidong Xiao

    IPC分类号： C12N15/35 ,  C12N7/06

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10344 ,  C12N2710/10351 ,  C12N2710/16643 ,  C12N2710/16651 ,  C12N2710/24144 ,  C12N2750/14151 ,  C12N2750/14351 ,  C12N2760/20234

    摘要： A method for the production of a replication-deficient recombinant virus vector is disclosed. The replication-deficient recombinant virus vector has a recombinant virus genome with one or more defective viral genes. The method comprises infecting a host cell with a carrier virus having a carrier virus genome encoding one or more trans factors or variants thereof, incubating the infected host cell for a desired period of time, and isolating the replication-deficient recombinant virus vector. The carrier virus is a cytoplasmic virus that retains the carrier virus genome in the cytoplasm of the host cell. The host cell contains the recombinant viral genome and retains the recombinant viral genome in a nucleus of the host cell. Also disclosed is a carrier virus for the production of a replication-deficient recombinant virus vector.

    摘要翻译： 公开了一种制备复制缺陷型重组病毒载体的方法。 复制缺陷型重组病毒载体具有带有一个或多个缺陷病毒基因的重组病毒基因组。 该方法包括用载体病毒感染宿主细胞，所述载体病毒具有编码一种或多种反式因子或其变体的载体病毒基因组，将所感染的宿主细胞孵育所需时间，并分离复制缺陷型重组病毒载体。 载体病毒是将载体病毒基因组保留在宿主细胞的细胞质中的细胞质病毒。 宿主细胞含有重组病毒基因组，并将重组病毒基因组保留在宿主细胞的细胞核中。 还公开了用于产生复制缺陷型重组病毒载体的载体病毒。

    Solar powered battery charger using switch capacitor voltage converters
    9.
    发明申请
    Solar powered battery charger using switch capacitor voltage converters 审中-公开
    太阳能电池充电器采用开关电容电压转换器

    公开(公告)号：US20090079385A1

    公开(公告)日：2009-03-26

    申请号：US12283962

    申请日：2008-09-17

    申请人： Weidong Xiao ,  Timothy James Roddick ,  Jason Leonard Scultety

    发明人： Weidong Xiao ,  Timothy James Roddick ,  Jason Leonard Scultety

    IPC分类号： H02J7/35

    CPC分类号： H02M3/07 ,  H02J7/35 ,  H02M3/1584 ,  Y02E10/566

    摘要： The invention provides a charging device that uses switched capacitor voltage converters to charge a battery using solar power. The charger uses boosting topology to efficiently use solar module photovoltaic power. The boosting topology enables a lower voltage to be used resulting in reduced cutting and soldering of photovoltaic cells. The battery charger has overcharge protection and uses inductor-less circuitry.

    摘要翻译： 本发明提供一种充电装置，其使用开关电容器电压转换器来对使用太阳能的电池进行充电。 充电器使用升压拓扑来有效利用太阳能组件的光伏电力。 升压拓扑结构使得能够使用更低的电压，从而减少光伏电池的切割和焊接。 电池充电器具有过充电保护功能，并采用无电感电路。