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    LIPOSOMALLY ENCAPSULATED HYBRID ADENOVIRUS-SEMLIKI FOREST VIRUS (SFV) VECTORS CARRYING RNAI CONSTRUCTS AND THERAPEUTIC GENES FOR USE AGAINST CANCER TARGETS AND OTHER DISEASES
    4.
    发明申请
    LIPOSOMALLY ENCAPSULATED HYBRID ADENOVIRUS-SEMLIKI FOREST VIRUS (SFV) VECTORS CARRYING RNAI CONSTRUCTS AND THERAPEUTIC GENES FOR USE AGAINST CANCER TARGETS AND OTHER DISEASES 审中-公开
    携带RNAI构建体和治疗癌基因用于癌症目标和其他疾病的感染性混合性ADENOVIRUS-SEMLIKI FOREST VIRUS（SFV）载体

    公开(公告)号：US20100008977A1

    公开(公告)日：2010-01-14

    申请号：US12439636

    申请日：2007-09-03

    申请人： Parthenios Boulikas ,  Michael Roberts

    发明人： Parthenios Boulikas ,  Michael Roberts

    IPC分类号： A61K9/127 ,  C12N15/00 ,  A61K35/76

    CPC分类号： C12N7/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2770/36143

    摘要： A hybrid adenovirus Semliki Forest Virus (SFV) vector includes 3′ and 5′ inverted terminal repeat (ITR) of adenovirus, the packaging signal of adenovirus, the structural genes encoding the adenovirus hexon and penton proteins, fiber and knob proteins and that may be deleted in the E4 region, E2 region or in the both the E2 and E4 regions. The adenovirus vector may not require a helper virus coinfection for propagation in producer cell lines. A hybrid vector includes a eukaryotic promoter controlling expression of the 42S genome of SFV comprising the nonstructural genes 1-4 or two point mutations thereof, and the therapeutic mRNA, in the cytoplasm. In use, the hybrid vector further comprises cDNA encoding for microRNA (miRNA) and hairpin loops of short interfering RNA (siRNA) or cDNA encoding for double-stranded RNA (dsRNA).

    摘要翻译： 杂合腺病毒塞立利森林病毒（SFV）载体包括腺病毒的3'和5'反向末端重复（ITR），腺病毒的包装信号，编码腺病毒六邻体和penton蛋白的结构基因，纤维和旋钮蛋白，并且可以是 在E4区域，E2区域或E2和E4区域都被删除。 腺病毒载体可能不需要辅助病毒共感染用于在生产细胞系中繁殖。 杂交载体包括控制包含非结构基因1-4或其两个点突变的SFV的42S基因组表达的真核启动子和细胞质中的治疗性mRNA。 在使用中，杂交载体还包含编码微小RNA（miRNA）的cDNA和短干扰RNA（siRNA）的发夹环或编码双链RNA（dsRNA）的cDNA。

    Hybrid adeno-retroviral vector for the transfection of cells
    5.
    发明授权
    Hybrid adeno-retroviral vector for the transfection of cells 失效
    用于转染细胞的杂交腺病毒载体

    公开(公告)号：US07618623B2

    公开(公告)日：2009-11-17

    申请号：US11255059

    申请日：2005-10-19

    申请人： Changyu Zheng ,  Brian O'Connell ,  Bruce J. Baum

    发明人： Changyu Zheng ,  Brian O'Connell ,  Bruce J. Baum

    IPC分类号： A61K48/00 ,  A01N63/00 ,  A01N43/04 ,  A61K31/711 ,  C12N15/861 ,  C12N15/867 ,  C12N15/33

    CPC分类号： C12N15/86 ,  A61K35/13 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2740/13043 ,  C12N2830/60

    摘要： An adenovirus, including adenoviral capsid proteins, and a replication-defective adenoviral vector that includes a 5′ retroviral LTR nucleic acid sequence, a 3′ retroviral LTR nucleic acid sequence, a nucleic acid sequence encoding a portion of a retroviral envelope protein adjacent to either the 5′ LTR or the 3′ LTR nucleic acid sequence, a retroviral packaging sequence and a nucleic acid sequence encoding a transgene located between the 5′ LTR and the 3′ LTR is provided. Host cells infected with this adenovirus are also provided. An adenoviral vector is provided that includes an adenoviral polynucleotide sequence comprising a nucleic acid encoding a transgene, a retroviral packaging signal, a 5′ and a 3′ retroviral LTR, and a portion of a retroviral envelope polypeptide, wherein the adenoviral polynucleotide sequence does not encode one or more of E1, E3 or E4. A method for transforming a cell is also provided using a virus or a vector of the invention, as is a method for introducing a transgene into a cell that is not able to produce viral particles with a single viral vector. A method is also provided for preventing or treating disorder in a subject using the adenoviral vectors of the invention. A pharmaceutical composition is also provided that includes an adenoviral vector of the invention and a pharmaceutically acceptable carrier.

    摘要翻译： 腺病毒包括腺病毒衣壳蛋白和复制缺陷型腺病毒载体，其包含5'逆转录病毒LTR核酸序列，3'逆转录病毒LTR核酸序列，编码逆转录病毒包膜蛋白部分的核酸序列， 提供5'LTR或3'LTR核酸序列，逆转录病毒包装序列和编码位于5'LTR和3'LTR之间的转基因的核酸序列。 还提供了感染该腺病毒的宿主细胞。 提供了包括腺病毒多核苷酸序列的腺病毒载体，其包含编码转基因的核酸，逆转录病毒包装信号，5'和3'逆转录病毒LTR以及部分逆转录病毒包膜多肽，其中所述腺病毒多核苷酸序列不 编码E1，E3或E4中的一个或多个。 还使用本发明的病毒或载体提供转化细胞的方法，以及将转基因导入到不能用单一病毒载体产生病毒颗粒的细胞中的方法。 还提供了使用本发明的腺病毒载体预防或治疗受试者的疾病的方法。 还提供了包含本发明的腺病毒载体和药学上可接受的载体的药物组合物。

    Production of recombinant AAV using adenovirus comprising AAV rep/cap genes
    7.
    发明申请
    Production of recombinant AAV using adenovirus comprising AAV rep/cap genes 审中-公开
    使用包含AAV rep / cap基因的腺病毒生产重组AAV

    公开(公告)号：US20070065412A1

    公开(公告)日：2007-03-22

    申请号：US11509855

    申请日：2006-08-25

    申请人： Haifeng Chen ,  Gary Kurtzman

    发明人： Haifeng Chen ,  Gary Kurtzman

    IPC分类号： A61K48/00 ,  C12N15/861

    CPC分类号： C12N15/86 ,  A61K2039/5256 ,  C12N2710/10344 ,  C12N2750/14143 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/75 ,  C12N2840/20 ,  C12N2840/203

    摘要： This invention relates to novel adenoviruses useful in the production of high titers of recombinant adeno-associated virus (rAAV) comprising a foreign DNA inert and methods of making these adenoviruses. The adenovirus comprises the AAV rep gene in which the p5 promoter of rep is replaced by a minimal promoter or by no promoter. The invention also provides methods of producing high levels of rAAV as a substantially homogeneous preparation and compositions of rAAV.

    摘要翻译： 本发明涉及可用于产生包含外源DNA惰性的重组腺相关病毒（rAAV）的高效价的新型腺病毒和制备这些腺病毒的方法。 腺病毒包含AAV rep基因，其中rep的p5启动子被最小启动子或没有启动子替代。 本发明还提供了生产高水平rAAV作为基本上均匀的制剂和rAAV的组合物的方法。

    Methods of generating chimeric adenoviruses and uses for such chimeric aden oviruses
    8.
    发明申请
    Methods of generating chimeric adenoviruses and uses for such chimeric aden oviruses 有权
    产生嵌合腺病毒的方法和用于这种嵌合腺病毒的方法

    公开(公告)号：US20060211115A1

    公开(公告)日：2006-09-21

    申请号：US10561201

    申请日：2004-06-15

    申请人： Soumitra Roy ,  James Wilson

    发明人： Soumitra Roy ,  James Wilson

    IPC分类号： C12N15/861 ,  C12N7/00

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2710/10351 ,  C12N2760/14134 ,  C12N2810/6018

    摘要： A method for providing an adenovirus from a serotype which does not grow efficiently in a desired cell line with the ability to grow in that cell line is described. The method involves replacing the left and right termini of the adenovirus with the corresponding termini from an adenovirus which grow efficiently in the desired cell line. At a minimum, the left terminus spans the (5′) inverted terminal repeat, the left terminus spans the E4 region and the (3′) inverted terminal repeat. The resulting chimeric adenovirus contains the internal regions spanning the genes encoding the penton, hexon and fiber from the serotype which does not grow efficiently in the desired cell. Also provided are vectors constructed from novel simian adenovirus sequences and proteins, host cells containing same, and uses thereof.

    摘要翻译： 描述了从在该细胞系中具有生长能力的所需细胞系中不能有效生长的血清型提供腺病毒的方法。 该方法包括用在期望的细胞系中有效生长的腺病毒的相应末端替换腺病毒的左侧和右侧末端。 至少，左终点跨越（5'）反向末端重复，左终点跨越E4区域，（3'）反向末端重复。 所得到的嵌合腺病毒包含跨越编码来自血清型的五邻体，六邻体和纤维的基因的内部区域，其不能在期望的细胞中有效生长。 还提供了由新型猿猴腺病毒序列和蛋白构成的载体，含有其的宿主细胞及其用途。