利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

57 条记录 (耗时 0.037 秒)

    Target proteins for eukaryotic tyrosine kinases
    4.
    发明授权
    Target proteins for eukaryotic tyrosine kinases 失效
    用于真核酪氨酸激酶的靶蛋白

    公开(公告)号：US5677421A

    公开(公告)日：1997-10-14

    申请号：US208887

    申请日：1994-03-11

    申请人： Joseph Schlessinger ,  Edward Y. Skolnik ,  Benjamin L. Margolis

    发明人： Joseph Schlessinger ,  Edward Y. Skolnik ,  Benjamin L. Margolis

    IPC分类号： A61K38/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/705 ,  C07K14/71 ,  C07K16/28 ,  C12N15/12

    CPC分类号： C07K14/4705 ,  C07K14/475 ,  C07K14/705 ,  C07K14/71 ,  C07K16/2863 ,  A61K38/00 ,  C07K2319/00 ,  G01N2333/62 ,  G01N2333/72 ,  G01N2333/9121

    摘要： A novel expression cloning method is provided for the detection, identification and purification of target proteins capable of binding at least to a tryosine-phosphorylated domain of a eukaryotic tyrosine kinase using novel peptide probes comprising an amino acid sequence substantially corresponding to a portion of a tyrosine-phosphorylated domain of a tyrosine kinase. The probe has at least one phosphorlated tyrosine residue and may be detectably labeled. Also disclosed is a method for preparing the probe, a method for mapping to a chormosome a gene encoding a protein capable of binding to tyrosine-phosphorylated domains of tyrosine kinases, and a method for purifiying such a protein with the probe. Non-limiting examples of novel proteins/discovered using the above cloning method include GRB-1, GRB-2, GRB-3, GRB-4 and GRB-7, as well as nucleic acid encoding these proteins, and methods for detecting these proteins are also provided.

    摘要翻译： 提供新的表达克隆方法用于检测，鉴定和纯化能够至少结合至真核细胞酪氨酸激酶的肌苷磷酸化结构域的靶蛋白，其使用包含基本上对应于酪氨酸的一部分的氨基酸序列的新型肽探针 酪氨酸激酶的磷酸化结构域。 探针具有至少一个磷酸化酪氨酸残基并且可以被可检测地标记。 还公开了一种制备探针的方法，用于将编码能够结合酪氨酸激酶的酪氨酸磷酸化结构域的蛋白质编码到基质体的方法，以及用该探针纯化这种蛋白质的方法。 使用上述克隆方法发现的新蛋白质的非限制性实例包括GRB-1，GRB-2，GRB-3，GRB-4和GRB-7以及编码这些蛋白质的核酸，以及用于检测这些蛋白质的方法 也提供。

    Methods and Compositions for Assessing and Treating Cancer
    7.
    发明申请
    Methods and Compositions for Assessing and Treating Cancer 审中-公开
    评估和治疗癌症的方法和组合

    公开(公告)号：US20130315934A1

    公开(公告)日：2013-11-28

    申请号：US13881623

    申请日：2011-10-28

    申请人： Ruth Halaban ,  Michael Krauthammer ,  Joseph Schlessinger ,  Titus J. Boggon

    发明人： Ruth Halaban ,  Michael Krauthammer ,  Joseph Schlessinger ,  Titus J. Boggon

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C07K14/4722 ,  C12N9/14 ,  G01N33/5743 ,  G01N2333/82 ,  G01N2800/52

    摘要： The invention relates to methods and compositions for identifying mutations associated with cancer, such as melanoma. More particularly, the invention relates to methods and compositions for assessing particular mutations as markers for cancer, such as melanoma. Such methods and compositions allow medical providers to diagnose cancer, such as melanoma, based upon the presence of one or more particular mutations in a subject, as compared with an otherwise healthy control. The methods and compositions are also useful in guiding the type of therapy to be applied, and monitoring the effects of the therapy applied.

    摘要翻译： 本发明涉及用于鉴定与癌症相关的突变的方法和组合物，例如黑素瘤。 更具体地，本发明涉及用于评估特定突变作为癌症标志物的方法和组合物，例如黑素瘤。 与其他健康对照相比，这样的方法和组合物允许医疗提供者基于受试者中一种或多种特定突变的存在来诊断癌症，例如黑素瘤。 方法和组合物也可用于指导所应用的治疗类型，并监测所施用的治疗效果。

    Methods for treatment or diagnosis of disease or disorders associated with an APB domain
    9.
    发明申请
    Methods for treatment or diagnosis of disease or disorders associated with an APB domain 审中-公开
    用于治疗或诊断与APB结构域相关的疾病或病症的方法

    公开(公告)号：US20050032689A1

    公开(公告)日：2005-02-10

    申请号：US10704128

    申请日：2003-11-10

    申请人： Benjamin Margolis ,  Joseph Schlessinger ,  Vijay Yajnik

    发明人： Benjamin Margolis ,  Joseph Schlessinger ,  Vijay Yajnik

    IPC分类号： A61K38/00 ,  C07K14/71 ,  G01N33/53

    CPC分类号： C07K14/71 ,  A61K38/00 ,  C07K2319/00

    摘要： The present invention concerns methods for diagnosis and treatment of diseases or disorders characterized by abnormal cellular signal transduction involving a newly identified region, herein termed the “APB domain.” APB domain binding between proteins is believed to play an important role in signal transduction pathways and, thereby, influence cellular events. Thus, APB mediated activity plays a role in signal transduction pathways and agents modulating APB mediated activity can be used to treat diseases or disorders involving proteins containing APB domains.

    摘要翻译： 本发明涉及用于诊断和治疗特征在于涉及新鉴定区域的异常细胞信号转导的疾病或病症的方法，这里称为“APB结构域”。 认为蛋白质之间的APB结构域结合在信号转导途径中起重要作用，从而影响细胞事件。 因此，APB介导的活性在信号转导途径中起作用，并且调节APB介导的活性的试剂可用于治疗涉及含有APB结构域的蛋白质的疾病或病症。

    Novel receptor-type phosphotyrosine phosphatase-alpha
    10.
    发明申请
    Novel receptor-type phosphotyrosine phosphatase-alpha 审中-公开
    新型受体型磷酸酪氨酸磷酸酶-α

    公开(公告)号：US20050032087A1

    公开(公告)日：2005-02-10

    申请号：US10777144

    申请日：2004-02-13

    申请人： Joseph Schlessinger ,  Jan Sap

    发明人： Joseph Schlessinger ,  Jan Sap

    IPC分类号： C07K14/705 ,  C12N9/16 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C07K14/705 ,  C07K2319/00 ,  C12N9/16

    摘要： A novel receptor-type protein tyrosine phosphatase (RPTP) protein or glycoprotein and the DNA coding therefor is expressed in a wide variety of mammalian tissues. Included in this family of proteins are human RPTPα, human RPTPβ and human RPTPγ. The RPTP protein or glycoprotein may be produced by recombinant means. Antibodies to the proteins, methods for measuring the quantity of the proteins, methods for screening compounds, such as drugs, which can bind to the proteins and inhibit or stimulate their activity, are provided.

    摘要翻译： 新型受体型蛋白酪氨酸磷酸酶（RPTP）蛋白或糖蛋白及其编码的DNA在多种哺乳动物组织中表达。 这种蛋白质家族包括人类RPTPα，人RPTPbeta和人类RPTPgamma。 可以通过重组方法产生RPTP蛋白或糖蛋白。 提供了对蛋白质的抗体，用于测量蛋白质量的方法，用于筛选可结合蛋白质并抑制或刺激其活性的化合物（例如药物）的方法。