公开(公告)号：US20240352401A1

公开(公告)日：2024-10-24

申请号：US18521797

申请日：2023-11-28

Applicant: Korea Institute of Science and Technology

Inventor： Sun Mi LEE ,  Jiwon Kim ,  Hyeoncheol Son ,  Ja Kyong Ko ,  Gyeongtaek Gong ,  Jung Ho Ahn ,  Youngsoon Um

IPC: C12N1/16 ,  C12N9/02 ,  C12N15/81

CPC classification number: C12N1/165 ,  C12N9/0008 ,  C12N15/815 ,  C12R2001/645 ,  C12Y102/01003

Abstract: The present disclosure discloses a strain having resistance by neutralizing furfural, an inhibitor derived from lignocellulosic biomass, based on a technique using aldehyde dehydrogenase and a method of using the same. According to the present disclosure, furfural, an inhibitor derived from lignocellulosic biomass, is neutralized based on a technique using aldehyde dehydrogenase, so that a resistant strain overexpresses aldehyde dehydrogenase (EcAldH) in Yarrowia lipolytica, which has the ability to produce yeast oil, and has resistance to furfural, a biomass-derived inhibitor. Accordingly, even in the medium containing furfural, it can provide excellent strain growth and yeast oil production ability.

公开(公告)号：US11285492B2

公开(公告)日：2022-03-29

申请号：US16393967

申请日：2019-04-25

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Yong-Sang Ryu ,  Eui-Sang Yu ,  Jiwon Kim ,  Ja Kyong Ko ,  Gyeongtaek Gong ,  Chulki Kim ,  Jae Hun Kim

IPC: B03C5/00 ,  C12P7/64 ,  C12P7/649

Abstract: Disclosed herein are a screening method of high-efficiency biofuel-producing strains by a dielectrophoretic method using vertical nano-gap electrodes and a producing method of biofuel from the screened strains.

公开(公告)号：US10913942B2

公开(公告)日：2021-02-09

申请号：US16204020

申请日：2018-11-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Sangdo Yook ,  Youngsoon Um ,  Gyeongtaek Gong ,  Jiwon Kim

IPC: C12N9/92 ,  C12N1/16 ,  C12P7/64 ,  C12N9/12 ,  C12N15/01 ,  C12N15/52 ,  C07K14/39

Abstract: Disclosed herein are a yeast strain capable of utilizing xylose as a carbon source and a method for producing lipids using the same. The yeast strain is obtained by adaptively evolving a wild-type yeast strain which cannot utilize xylose as a carbon source so that it can produce high density lipids and then transforming the adaptively evolved strain to obtain the ability to metabolize xylose. Since the strain does not have the xylose metabolic pathway based on oxidoreductase, it can produce biodiesel and biomaterials based on lipid and lignocellulosic biomass at a high yield without a problem of cofactor imbalance and can greatly improve the economic feasibility and sustainability of the production processes of biodiesel and biomaterials.