公开(公告)号：US20160265028A1

公开(公告)日：2016-09-15

申请号：US15032541

申请日：2014-10-28

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Kyung KIM ,  Nakwon CHOI ,  Dong Jin LEE ,  Seungwon JUNG

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01J2219/00317 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01L3/50851 ,  B01L2200/16 ,  B01L2300/069 ,  B01L2300/0819 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/519

Abstract: A porous structure according to the present invention has a polymerase chain reaction (PCT) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time PCR.

Abstract translation: 根据本发明的多孔结构在其孔内具有聚合酶链式反应（PCT）引物，因此，与其仅使用表面用于扩增和检测的一般结构相比，甚至可以使用其内部部分，从而使反应性最大化。 此外，各结构中所含的引物种类的差异导致同时检测多种目标核酸并进行实时分析，因此可用于多重实时PCR。

公开(公告)号：US20170321265A1

公开(公告)日：2017-11-09

申请号：US15499005

申请日：2017-04-27

Applicant: Korea Institute of Science and Technology

Inventor： Sang Kyung KIM ,  Seungwon JUNG

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2563/157

Abstract: A porous matrix according to the present disclosure, wherein a nucleic acid primer-carbon material composite in which one or more nucleic acid primer of a forward primer and a reverse primer as a polymerase chain reaction (PCR) primer is bound to a carbon material is included in the pores of the matrix, provides improved amplification efficiency as compared to a matrix wherein the nucleic acid primer is present on the outer surface of the matrix or a porous matrix wherein the nucleic acid primer is directly fixed inside pores. The porous matrix of the present disclosure can effectively detect various kinds of target nucleic acids simultaneously and analyze them in real time by varying the kinds of the nucleic acid primers included in the matrix. Therefore, it is useful in amplifying multiple nucleic acids.