公开(公告)号：US20160068816A1

公开(公告)日：2016-03-10

申请号：US14783382

申请日：2014-04-14

Applicant: Kyoto University

Inventor： Kenji OSAFUNE ,  Shimpei GOTOH ,  Isao ITO ,  Michiaki MISHIMA

IPC: C12N5/071

CPC classification number: C12N5/0688 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/065 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/45

Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3β inhibitor, (2) a medium containing a BMP inhibitor and a TGFβ inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3β inhibitor.

Abstract translation: 本发明提供了一种从多能干细胞稳定产生肺泡上皮祖细胞的方法，包括在（1）含有激活素A和GSK3和bgr的培养基中培养多能干细胞的步骤; 抑制剂，（2）含有BMP抑制剂和TGFβb的培养基; 抑制剂，和（3）含有BMP4，视黄酸和GSK3＆bgr的培养基; 抑制剂。

公开(公告)号：US20210284968A1

公开(公告)日：2021-09-16

申请号：US17194761

申请日：2021-03-08

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Shimpei GOTOH ,  Isao ITO ,  Michiaki MISHIMA

IPC: C12N5/071

Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3β inhibitor, (2) a medium containing a BMP inhibitor and a TGFβ inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3β inhibitor.