公开(公告)号：US20220186189A1

公开(公告)日：2022-06-16

申请号：US17653185

申请日：2022-03-02

Applicant: KYOTO UNIVERSITY

Inventor： Shimpei GOTOH ,  Yuki YAMAMOTO ,  Satoshi KONISHI ,  Michiaki MISHlMA

IPC: C12N5/071

Abstract: This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3β inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.

公开(公告)号：US20210284968A1

公开(公告)日：2021-09-16

申请号：US17194761

申请日：2021-03-08

Applicant: KYOTO UNIVERSITY

Inventor： Kenji OSAFUNE ,  Shimpei GOTOH ,  Isao ITO ,  Michiaki MISHIMA

IPC: C12N5/071

Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3β inhibitor, (2) a medium containing a BMP inhibitor and a TGFβ inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3β inhibitor.

公开(公告)号：US20180112186A1

公开(公告)日：2018-04-26

申请号：US15558670

申请日：2016-03-18

Applicant: KYOTO UNIVERSITY

Inventor： Shimpei GOTOH ,  Yuki YAMAMOTO ,  Satoshi KONISHI ,  Michiaki MISHlMA

IPC: C12N5/071

CPC classification number: C12N5/0688 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/45

Abstract: This invention provides a method for stably producing airway epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing airway epithelial cells from pluripotent stem cells comprising steps: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (5) subjecting the cells obtained after Step (3) to three-dimensional culture in a medium containing a GSK3β inhibitor, FGF10, and a ROCK inhibitor; and (6) subjecting the proximal airway epithelial progenitor cells obtained in Step (5) to three-dimensional culture in a medium containing a ROCK inhibitor.

公开(公告)号：US20250163385A1

公开(公告)日：2025-05-22

申请号：US18835286

申请日：2023-01-30

Applicant: KYOTO UNIVERSITY

Inventor： Shimpei GOTOH ,  Koji TAMAI

IPC: C12N5/071

Abstract: The present disclosure provides a method for producing lung mesenchymal cells that can be used as feeder cells in induction of alveolar epithelial cells. A method for producing lung mesenchymal cells of the present disclosure includes culturing mesodermal cells in a presence of a mesenchymal cell-inducing factor, KGF, and FGF10 so as to induce differentiation of the mesodermal cells into lung mesenchymal cells.

公开(公告)号：US20180051256A1

公开(公告)日：2018-02-22

申请号：US15555183

申请日：2016-03-02

Applicant: KYOTO UNIVERSITY

Inventor： Shimpei GOTOH ,  Yuki YAMAMOTO ,  Satoshi KONISHI ,  Michiaki MISHlMA

IPC: C12N5/071

CPC classification number: C12N5/0688 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2506/45 ,  C12N2513/00

Abstract: This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3β inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.

公开(公告)号：US20160068816A1

公开(公告)日：2016-03-10

申请号：US14783382

申请日：2014-04-14

Applicant: Kyoto University

Inventor： Kenji OSAFUNE ,  Shimpei GOTOH ,  Isao ITO ,  Michiaki MISHIMA

IPC: C12N5/071

CPC classification number: C12N5/0688 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/065 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/45

Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3β inhibitor, (2) a medium containing a BMP inhibitor and a TGFβ inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3β inhibitor.

Abstract translation: 本发明提供了一种从多能干细胞稳定产生肺泡上皮祖细胞的方法，包括在（1）含有激活素A和GSK3和bgr的培养基中培养多能干细胞的步骤; 抑制剂，（2）含有BMP抑制剂和TGFβb的培养基; 抑制剂，和（3）含有BMP4，视黄酸和GSK3＆bgr的培养基; 抑制剂。