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公开(公告)号：US06962796B1
公开(公告)日：2005-11-08
申请号：US09668154
申请日：2000-09-25
IPC分类号： C12N15/09 , A61K38/00 , C07K14/575 , C07K14/635 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/16 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/865
CPC分类号： C07K14/635 , A61K38/00 , C07K2319/00 , C07K2319/02 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
摘要翻译： 本发明提供含有编码人前甲状腺激素的DNA序列的重组质粒。 本发明还提供了由这些质粒转化的微生物，例如大肠杆菌。 本发明还提供了用于插入酵母的质粒和其中质粒含有编码甲状旁腺激素的DNA的转化酵母。 甲状旁腺激素然后被转化的酵母分泌。 此外，本发明提供了具有甲状旁腺激素活性的替代多肽，包括PTH类似物，片段和延伸，并提供可用于本发明的替代前导序列和分泌信号序列。 最后，提供了分泌的PTH激素和/或衍生物的纯化方法。
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公开(公告)号：US6146852A
公开(公告)日：2000-11-14
申请号：US461436
申请日：1995-06-05
IPC分类号： A61K38/00 , C07K14/635 , C12N15/16 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81
CPC分类号： C07K14/635 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , A61K38/00 , C07K2319/02
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
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公开(公告)号：US5420242A
公开(公告)日：1995-05-30
申请号：US087471
申请日：1993-07-02
申请人： Kaare M. Gautvik , Peter Alestrom , Tordis B. Oyen
发明人： Kaare M. Gautvik , Peter Alestrom , Tordis B. Oyen
IPC分类号： A61K38/00 , C07K14/635 , C12N15/16 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , C07K14/585
CPC分类号： C07K14/635 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , A61K38/00 , C07K2319/02
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
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公开(公告)号：US5010010A
公开(公告)日：1991-04-23
申请号：US393851
申请日：1989-08-14
CPC分类号： C07K14/635 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , C07K2319/02
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. Finally, the invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast.
摘要翻译： 本发明提供含有编码人前甲状腺激素的DNA序列的重组质粒。 本发明还提供了由这些质粒转化的微生物，例如大肠杆菌。 最后，本发明还提供了用于插入酵母和质粒含有编码甲状旁腺激素的DNA的转化酵母的质粒。 甲状旁腺激素然后被转化的酵母分泌。
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公开(公告)号：US20070010439A1
公开(公告)日：2007-01-11
申请号：US11247585
申请日：2005-10-12
申请人： Kaare Gautvik , Peter Alestrom , Tordis Oyen , Odd Gabrielsen
发明人： Kaare Gautvik , Peter Alestrom , Tordis Oyen , Odd Gabrielsen
IPC分类号： A61K38/29 , C12N1/18 , C07K14/635
CPC分类号： C07K14/635 , A61K38/00 , C07K2319/02 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
摘要翻译： 本发明提供含有编码人前甲状腺激素的DNA序列的重组质粒。 本发明还提供了由这些质粒转化的微生物，例如大肠杆菌。 本发明还提供了用于插入酵母的质粒和其中质粒含有编码甲状旁腺激素的DNA的转化酵母。 甲状旁腺激素然后被转化的酵母分泌。 此外，本发明提供了具有甲状旁腺激素活性的替代多肽，包括PTH类似物，片段和延伸，并提供可用于本发明的替代前导序列和分泌信号序列。 最后，提供了分泌的PTH激素和/或衍生物的纯化方法。
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