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公开(公告)号：US06962796B1
公开(公告)日：2005-11-08
申请号：US09668154
申请日：2000-09-25
IPC分类号： C12N15/09 , A61K38/00 , C07K14/575 , C07K14/635 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/16 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/865
CPC分类号： C07K14/635 , A61K38/00 , C07K2319/00 , C07K2319/02 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
摘要翻译： 本发明提供含有编码人前甲状腺激素的DNA序列的重组质粒。 本发明还提供了由这些质粒转化的微生物，例如大肠杆菌。 本发明还提供了用于插入酵母的质粒和其中质粒含有编码甲状旁腺激素的DNA的转化酵母。 甲状旁腺激素然后被转化的酵母分泌。 此外，本发明提供了具有甲状旁腺激素活性的替代多肽，包括PTH类似物，片段和延伸，并提供可用于本发明的替代前导序列和分泌信号序列。 最后，提供了分泌的PTH激素和/或衍生物的纯化方法。
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公开(公告)号：US6146852A
公开(公告)日：2000-11-14
申请号：US461436
申请日：1995-06-05
IPC分类号： A61K38/00 , C07K14/635 , C12N15/16 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81
CPC分类号： C07K14/635 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , A61K38/00 , C07K2319/02
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
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公开(公告)号：US5420242A
公开(公告)日：1995-05-30
申请号：US087471
申请日：1993-07-02
申请人： Kaare M. Gautvik , Peter Alestrom , Tordis B. Oyen
发明人： Kaare M. Gautvik , Peter Alestrom , Tordis B. Oyen
IPC分类号： A61K38/00 , C07K14/635 , C12N15/16 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , C07K14/585
CPC分类号： C07K14/635 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , A61K38/00 , C07K2319/02
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.
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公开(公告)号：US5010010A
公开(公告)日：1991-04-23
申请号：US393851
申请日：1989-08-14
CPC分类号： C07K14/635 , C12N15/64 , C12N15/66 , C12N15/67 , C12N15/70 , C12N15/81 , C07K2319/02
摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. Finally, the invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast.
摘要翻译： 本发明提供含有编码人前甲状腺激素的DNA序列的重组质粒。 本发明还提供了由这些质粒转化的微生物，例如大肠杆菌。 最后，本发明还提供了用于插入酵母和质粒含有编码甲状旁腺激素的DNA的转化酵母的质粒。 甲状旁腺激素然后被转化的酵母分泌。
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公开(公告)号：US06635479B1
公开(公告)日：2003-10-21
申请号：US09230896
申请日：1999-02-02
申请人： J. Gregor Sutcliffe , Kaare M. Gautvik , Luis De Lecea , Floyd E. Bloom , Patria E. Danielson , Vigdis T. Gautvik , Thomas S. Kilduff , Pamela E. Foye
发明人： J. Gregor Sutcliffe , Kaare M. Gautvik , Luis De Lecea , Floyd E. Bloom , Patria E. Danielson , Vigdis T. Gautvik , Thomas S. Kilduff , Pamela E. Foye
IPC分类号： C07H2104
CPC分类号： C07K14/5759 , A61K38/00
摘要： Disclosed are hypocretin polynucleotides and hypocretin polypeplides, as well as antibodies, oligonucleotides, diagnostic kits and methods, and therapeutic compositions and methods. Hypocretin, one of several novel liypothalamic-specific polypeptides identified isolated and sequenced, is localized to regions of the hypothalamus involved in appetite and feeding behavior. Hypocretin polypeptides are biologically active, producing electrical changes in neurons, lowering body temperature and reducing food intake.
摘要翻译： 公开了下丘脑多核苷酸和下丘脑多聚肽，以及抗体，寡核苷酸，诊断试剂盒和方法以及治疗组合物和方法。 识别分离和测序的几种新型下丘脑特异性多肽之一的下丘脑定位于涉及食欲和摄食行为的下丘脑区域。 下丘脑多肽具有生物活性，在神经元中产生电变化，降低体温并减少食物摄入。
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