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公开(公告)号：US4931391A
公开(公告)日：1990-06-05
申请号：US45869
申请日：1987-05-01
CPC分类号： C12N9/96 , C12N9/78 , Y10S435/832 , Y10S435/84 , Y10S435/843 , Y10S435/859 , Y10S435/872
摘要： Nitrile hydration activity of the nitrilase or the immobilized from thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.
摘要翻译： 通过向腈水解酶或其固定化形式的溶液或悬浮液中加入选自腈，酰胺和有机酸及其盐中的至少一种化合物作为稳定剂，可稳定地保留腈水解酶或其固定化合物的腈水合活性 。
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公开(公告)号：US4900672A
公开(公告)日：1990-02-13
申请号：US45870
申请日：1987-05-01
摘要： Nitrile hydration activity of nitrile hydratase produced by a microorganism of the genus Pseudomonas or Pseudomonas microorganism cells per se or in the immobilized state, all having nitrile hydration activity can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a suspension or solution of the nitrile hydratase, or the microorganism cells per se or in the immobilized state.
摘要翻译： 可以通过添加至少一种选自腈，酰胺等的化合物作为稳定剂，稳定地保持由假单胞菌属或假单胞菌属微生物细胞本身或固定化状态产生的腈水合酶的腈水合活性，全部具有腈水合活性， 腈水合酶或微生物细胞本身或固定化状态的悬浮液或溶液中的有机酸及其盐。
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公开(公告)号：US4661456A
公开(公告)日：1987-04-28
申请号：US569021
申请日：1984-01-09
CPC分类号： C12N9/78 , C12P13/02 , Y10S435/874
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one .alpha.-amino acid, except for cysteine or cystine alone and a combination of these .alpha.-amino acids alone, in the preparation of cells of bacteria having nitrile hydratase activity by cultivating under nitrile hydratase-inducing conditions Pseudomonas bacteria capable of producing nitrile hydratase.
摘要翻译： 通过向培养基中加入除了单独的半胱氨酸或胱氨酸以外的至少一种α-氨基酸和单独的这些α-氨基酸的组合，可以以高产率获得具有高腈水合酶活性的假单胞菌细菌的细胞 通过在腈水合酶诱导条件下培养能够产生腈水合酶的假单胞菌来制备具有腈水合酶活性的细菌细胞。
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公开(公告)号：US4555487A
公开(公告)日：1985-11-26
申请号：US551852
申请日：1983-11-15
申请人： Hideaki Yamada , Koitchiro Ryuno
发明人： Hideaki Yamada , Koitchiro Ryuno
CPC分类号： C12N9/78 , C12N1/20 , Y10S435/874
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one amide compound selected from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Pseudomonas bacteria capable of producing nitrile hydratase.
摘要翻译： 通过向培养基中加入选自丙烯酰胺，甲基丙烯酰胺，巴豆酰胺和正丁酰胺中的至少一种酰胺化合物，可以高产率地获得具有高腈水合酶活性的假单胞菌细菌的细胞， 通过培养能够产生腈水合酶的假单胞菌细菌具有腈水合酶活性的细菌。
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