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公开(公告)号：US4900672A
公开(公告)日：1990-02-13
申请号：US45870
申请日：1987-05-01
摘要： Nitrile hydration activity of nitrile hydratase produced by a microorganism of the genus Pseudomonas or Pseudomonas microorganism cells per se or in the immobilized state, all having nitrile hydration activity can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a suspension or solution of the nitrile hydratase, or the microorganism cells per se or in the immobilized state.
摘要翻译： 可以通过添加至少一种选自腈，酰胺等的化合物作为稳定剂，稳定地保持由假单胞菌属或假单胞菌属微生物细胞本身或固定化状态产生的腈水合酶的腈水合活性，全部具有腈水合活性， 腈水合酶或微生物细胞本身或固定化状态的悬浮液或溶液中的有机酸及其盐。
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公开(公告)号：US4661456A
公开(公告)日：1987-04-28
申请号：US569021
申请日：1984-01-09
CPC分类号： C12N9/78 , C12P13/02 , Y10S435/874
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one .alpha.-amino acid, except for cysteine or cystine alone and a combination of these .alpha.-amino acids alone, in the preparation of cells of bacteria having nitrile hydratase activity by cultivating under nitrile hydratase-inducing conditions Pseudomonas bacteria capable of producing nitrile hydratase.
摘要翻译： 通过向培养基中加入除了单独的半胱氨酸或胱氨酸以外的至少一种α-氨基酸和单独的这些α-氨基酸的组合，可以以高产率获得具有高腈水合酶活性的假单胞菌细菌的细胞 通过在腈水合酶诱导条件下培养能够产生腈水合酶的假单胞菌来制备具有腈水合酶活性的细菌细胞。
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公开(公告)号：US4931391A
公开(公告)日：1990-06-05
申请号：US45869
申请日：1987-05-01
CPC分类号： C12N9/96 , C12N9/78 , Y10S435/832 , Y10S435/84 , Y10S435/843 , Y10S435/859 , Y10S435/872
摘要： Nitrile hydration activity of the nitrilase or the immobilized from thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.
摘要翻译： 通过向腈水解酶或其固定化形式的溶液或悬浮液中加入选自腈，酰胺和有机酸及其盐中的至少一种化合物作为稳定剂，可稳定地保留腈水解酶或其固定化合物的腈水合活性 。
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公开(公告)号：US4880739A
公开(公告)日：1989-11-14
申请号：US104566
申请日：1987-10-02
CPC分类号： C12P13/02 , C12N9/78 , Y10S435/874
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding sequentially to a culture medium at least one compound selected from the group consisting of propionitrile, isobutyronitrile, propionamide, and isobutyramide in the process of cultivation of Pseudomonas bacteria capable of producing nitrile hydratase.
摘要翻译： 在培养假单胞菌的过程中，可以通过向培养基中依次加入至少一种选自丙腈，异丁腈，丙酰胺和异丁酰胺的化合物，从而以高产率获得具有高腈水合酶活性的假单胞菌细菌的细胞 能够生产腈水合酶。
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公开(公告)号：US4661457A
公开(公告)日：1987-04-28
申请号：US569047
申请日：1984-01-09
CPC分类号： C12N9/78 , C12P13/02 , Y10S435/874
摘要： Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium cysteine and (or) cystine in the preparation of cells of bacteria having nitrile hydratase activity by cultivating under nitrile hydratase-inducing conditions Pseudomonas bacteria capable of producing nitrile hydratase.
摘要翻译： 通过在腈水合酶诱导条件下培养制备具有腈水合酶活性的细菌细胞的培养基半胱氨酸和（或）胱氨酸可以高产率获得具有高腈水合酶活性的细菌的细胞。假单胞菌 能够生产腈水合酶。
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公开(公告)号：US06238896B1
公开(公告)日：2001-05-29
申请号：US09367716
申请日：1999-08-20
申请人： Eiji Ozaki , Kanehiko Enomoto , Takakazu Endo
发明人： Eiji Ozaki , Kanehiko Enomoto , Takakazu Endo
IPC分类号： C12P762
CPC分类号： C12R1/15 , C12P7/62 , C12R1/01 , C12R1/365 , Y10S435/822 , Y10S435/843
摘要： The present invention relates to a method for preparing a malonic acid monoester represented by Formula (II): HOOCCH2COOR (II) wherein R is alkenyl, aryl, aralkyl or C1-20 alkyl, comprising treating a cyanoacetic acid ester represented by Formula (I): NCCH2COOR (I) wherein R is defined in Formula (II), with a culture, cells or a product from treated cells of a microorganism belonging to the genus Corynebacterium, Gordona or Rhodococcus and having nitrilase activity to thereby hydrolyze the cyanoacetic acid ester.
摘要翻译： 本发明涉及一种制备由式（II）表示的丙二酸单酯的方法：其中R是烯基，芳基，芳烷基或C 1-20烷基，包括处理由式（I）表示的氰基乙酸酯：其中R是 与式（II）中定义的具有来自属于棒杆菌属，戈多纳或红球菌属的微生物的处理细胞的培养物，细胞或产物，并且具有腈水解酶活性，从而水解氰基乙酸酯。
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7.发明授权
公开(公告)号：US5071753A
公开(公告)日：1991-12-10
申请号：US607676
申请日：1990-11-01
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： A process for the preparation of an organic ester of ascorbic acid or erythorbic acid represented by the general formula (II) or (III): ##STR1## wherein R.sub.1 is alkyl, aralkyl, or aryl, by reacting ascorbic acid or erythorbic acid with an organic acid or an ester thereof of the general formula (I):wherein R.sub.1 is as defined above and R.sub.2 is hydrogen, methyl, ethyl or propyl, in an organic solvent in the presence of an ester hydrolase.
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公开(公告)号：US5149855A
公开(公告)日：1992-09-22
申请号：US627779
申请日：1990-12-14
IPC分类号： C07C327/32
CPC分类号： C07C327/32
摘要： Process for racemizing an optically active carboxylic acid ester of the formula (1): ##STR1## wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 independently are alkyl, and n is 1 or 2, which comprises contacting the compound of the formula (1) with an amine compound.
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公开(公告)号：US5149649A
公开(公告)日：1992-09-22
申请号：US561323
申请日：1990-08-01
摘要： A method for culturing cells by supplying oxygen to a culture medium through a multilayer composite membrane consisting of a porous layer(s) and a nonporous layer(s) having a thickness of less than 10 micrometers laminated one after the other. By using this multilayer composite membrane for supplying oxygen to the culture medium, oxygen can be supplied to the culture medium and/or the culture broth without causing foaming in the culture medium, cell growth inhibition or reduction in productivity of cellular products.
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公开(公告)号：US4908313A
公开(公告)日：1990-03-13
申请号：US131281
申请日：1987-12-10
IPC分类号： C12N1/20 , C12N13/00 , C12P1/04 , C12P13/02 , C12R1/01 , C12R1/07 , C12R1/13 , C12R1/15 , C12R1/265 , C12R1/365
摘要： In a process for hydrating a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive gram-staining microorganism, (b) the microbial cells are allowed to accept light energy of at least about 1.times.10.sup.-2 .mu.E/g microbial cells second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light transmitting material.
摘要翻译： 在通过具有腈水解酶活性的微生物的作用将腈化合物水合化以将腈化合物转化成相应的酰胺化合物的方法中，通过用光照射微生物，显着提高了产率和反应速度等性能。 本发明的方法的特征在于（a）具有腈水解酶活性的微生物是阳性革兰氏染色微生物，（b）允许微生物细胞接受至少约1×10-2微克/微克微生物 在第二次水合反应结束之前的细胞，和（c）水合反应在至少部分由不透光材料组成的容器中进行。
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