公开(公告)号：US20220348467A1

公开(公告)日：2022-11-03

申请号：US17620357

申请日：2020-06-17

Applicant: Kansas State University Research Foundation

Inventor： Stefan H. Bossmann ,  Christopher Sorensen ,  Jose Covarrubias ,  Madumali Kalubowilage ,  Arjun Nepal

IPC: C01B32/194 ,  C01B32/198 ,  C09D11/037 ,  C08K9/02 ,  C04B20/02

Abstract: Methods of preparing graphene/graphene oxide particulates under mild conditions, comprising reacting pristine graphene with hydrogen peroxide and a source of iron to oxidize the outer surface of the pristine graphene particulates in solution and yield graphene/graphene oxide particulates. Methods and articles incorporating the same are also disclosed.

公开(公告)号：US20170219548A1

公开(公告)日：2017-08-03

申请号：US15329395

申请日：2015-07-27

Applicant: Kansas State University Research Foundation

Inventor： Deryl L. Troyer ,  Stefan H. Bossmann ,  Aruni P. Malalasekera ,  Thilani N. Samarakoon ,  Hongwang Wang ,  Madumali Kalubowilage

IPC: G01N33/04 ,  C12Q1/37 ,  C12Q1/04 ,  C12Q1/44

CPC classification number: G01N33/04 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/44 ,  G01N2333/952

Abstract: Methods for detecting biomarkers of inflammation, infection, and/or bacterial activity in dairy production, which indicate issues with the milk itself or issues related to the health of the cow. The methods generally comprise contacting a milk sample with a nanoplatform assembly to create an assay solution, and detecting spectral changes in the assay solution that are triggered by enzymatic activity (when present) in the sample. The nanoplatform assembly comprises a first particle, a second particle, and a linkage therebetween, wherein the linkage comprises a protease consensus sequence (the sequence of amino acids cleaved by the protease), or an ester linkage (cleaved by a protease or lipase). A plurality of second particles can also be linked to the first particle. Test strips are also described, which undergo a visual color change in the presence of the target enzyme in the milk sample.

公开(公告)号：US20220024877A1

公开(公告)日：2022-01-27

申请号：US17277631

申请日：2019-09-20

Applicant: Kansas State University Research Foundation ,  The UAB Research Foundation

Inventor： Stefan H. Bossmann ,  Madumali Kalubowilage ,  Man Zhang ,  Anjana Delpe-Acharige ,  Kayla Eschliman ,  Frank Wolschendorf ,  Olaf Kutsch ,  Alex Dalecki ,  Whitney Narmore

IPC: C07D231/42 ,  C07D231/22 ,  A61K45/06 ,  A61P31/04

Abstract: Heterocyclic compounds with a novel pyrazole thioamide-based NNSN structural motif, having highly effective zinc- or copper-activated toxicity against microbial infections at micromolar or nanomolar minimum inhibitory concentrations (MIC), and methods of making and using same.

公开(公告)号：US10416144B2

公开(公告)日：2019-09-17

申请号：US15329395

申请日：2015-07-27

Applicant: Kansas State University Research Foundation

Inventor： Deryl L. Troyer ,  Stefan H. Bossmann ,  Aruni P. Malalasekera ,  Thilani N. Samarakoon ,  Hongwang Wang ,  Madumali Kalubowilage

IPC: G01N33/04 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/44

Abstract: Methods for detecting biomarkers of inflammation, infection, and/or bacterial activity in dairy production, which indicate issues with the milk itself or issues related to the health of the cow. The methods generally comprise contacting a milk sample with a nanoplatform assembly to create an assay solution, and detecting spectral changes in the assay solution that are triggered by enzymatic activity (when present) in the sample. The nanoplatform assembly comprises a first particle, a second particle, and a linkage therebetween, wherein the linkage comprises a protease consensus sequence (the sequence of amino acids cleaved by the protease), or an ester linkage (cleaved by a protease or lipase). A plurality of second particles can also be linked to the first particle. Test strips are also described, which undergo a visual color change in the presence of the target enzyme in the milk sample.