公开(公告)号：US20210317484A1

公开(公告)日：2021-10-14

申请号：US17269689

申请日：2019-09-06

申请人： Kao Corporation

发明人： Kyoshiro NONAKA ,  Fumikazu TAKAHASHI

IPC分类号： C12P13/00

摘要： Provided is a method for manufacturing a 3-hydroxy-4-aminobenzoic acid by using a microorganism. The method for manufacturing a 3-hydroxy-4-aminobenzoic acid comprises a step of bringing a 4-aminobenzoic acid into contact with a microorganism that produces the following polypeptide (A) or (B): (A) a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 2 or a polypeptide consisting of an amino acid sequence that has at least 90% identity to the amino acid sequence shown in SEQ ID NO: 2 and has 4-hydroxybenzoate hydroxylase activity, (B) a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 6 or a polypeptide consisting of an amino acid sequence that has at least 90% identity to the amino acid sequence shown in SEQ ID NO: 6 and has 4-hydroxybenzoate hydroxylase activity.

公开(公告)号：US20190284586A1

公开(公告)日：2019-09-19

申请号：US16463075

申请日：2017-11-27

申请人： Kao Corporation

发明人： Kyoshiro NONAKA

IPC分类号： C12P7/46 ,  C12N9/08

摘要： Provided are a filamentous fungus mutant strain having an effect of improving C4 dicarboxylic acid productivity, and a method for producing C4 dicarboxylic acid using the filamentous fungus mutant strain. The method for producing a C4 dicarboxylic acid comprises culturing a filamentous fungus mutant strain which has enhanced expression of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 or an amino acid sequence having at least 90% identity thereto and having catalase activity is enhanced.

公开(公告)号：US20240110211A1

公开(公告)日：2024-04-04

申请号：US17791377

申请日：2020-12-28

申请人： Kao Corporation

发明人： Kyoshiro NONAKA ,  Fumikazu TAKAHASHI

IPC分类号： C12P17/12 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N15/70 ,  C12P7/44

CPC分类号： C12P17/12 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/70 ,  C12P7/44 ,  C12Y113/11 ,  C12Y206/01 ,  C12Y401/03038 ,  C12N2800/101

摘要： Provided are a transformed cell having the ability to produce 2,5-pyridine dicarboxylic acids, and a method for producing 2,5-pyridine dicarboxylic acids using the same. The present invention provides a transformed cell having the ability to produce 2,5-pyridine dicarboxylic acids, the transformed cell being derived from a microbe having the ability to biosynthesize 4-aminobenzoic acids and having the enhanced expression of the following polypeptides (I) and (II): (I) a polypeptide having 4-aminobenzoic acid hydroxylation activity, and (II) a polypeptide having 4-amino-3-hydroxybenzoate 2,3-dioxygenase activity.

公开(公告)号：US20190316159A1

公开(公告)日：2019-10-17

申请号：US16313547

申请日：2017-06-22

申请人： Kao Corporation

发明人： Kyoshiro NONAKA ,  Fumikazu TAKAHASHI

IPC分类号： C12P7/46 ,  C12N1/14

摘要： Improvement in C4 dicarboxylic acid productivity in a host cell is provided. A polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2 or an amino acid sequence having at least 90% identity therewith.

公开(公告)号：US20190194700A1

公开(公告)日：2019-06-27

申请号：US16330173

申请日：2017-09-05

申请人： Kao Corporation

发明人： Kyoshiro NONAKA

IPC分类号： C12P7/46 ,  C12N1/14 ,  C12R1/845

CPC分类号： C12P7/46 ,  C12N1/14 ,  C12N15/09 ,  C12R1/845

摘要： Provided is a mutant filamentous fungus having improved C4 dicarboxylic acid productivity and a method for producing a C4 dicarboxylic acid using the mutant filamentous fungus. A mutant filamentous fungus having enhanced expression of at least one polypeptide selected from the group consisting of a polypeptide consisting of the amino acid sequence represented by SEQ ID No: 2; a polypeptide consisting of an amino acid sequence having an identity of at least 90% with the amino acid sequence represented by SEQ ID No: 2 and having malic enzyme activity; and a polypeptide consisting of an amino acid sequence having deletion, substitution, addition or insertion of one or more amino acids with respect to the amino acid sequence represented by SEQ ID No: 2 and having malic enzyme activity.