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    RHIZOPUS ORYZAE STRAIN, MUTAGENESIS AND SCREENING METHODS THEREOF, AND METHODS OF FERMENTING TO PRODUCE FUMARIC ACID
    3.
    发明申请
    RHIZOPUS ORYZAE STRAIN, MUTAGENESIS AND SCREENING METHODS THEREOF, AND METHODS OF FERMENTING TO PRODUCE FUMARIC ACID 有权
    红霉素ORYZAE菌株，MUTAGENESIS和筛选方法及其生产方法

    公开(公告)号：US20140004560A1

    公开(公告)日：2014-01-02

    申请号：US13992233

    申请日：2011-04-16

    申请人： He Huang ,  Shuang Li ,  Qing Xu ,  Zhen Gao

    发明人： He Huang ,  Shuang Li ,  Qing Xu ,  Zhen Gao

    IPC分类号： C12P7/46

    CPC分类号： C12P7/46 ,  C12N1/14 ,  C12N15/01 ,  C12R1/845

    摘要： A rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named as Rhizopus oryzae ME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying.

    摘要翻译： 根霉菌株，其诱变和筛选方法，以及通过发酵产生富马酸的方法。 该菌株被命名为米曲霉ME-F13，并存放于中国型号培养基中心，存放号码为CCTCC M 2010351.该菌株是通过离子注射物理诱变原始菌株ME-F12获得的， 含有2-D脱氧葡萄糖（2-DG）的固体选择性板和拾取2-DG抗性单个菌落。 该菌株能够同时糖化淀粉物质并将其发酵以产生富马酸。 具有改善的酶活性，该菌株可以直接用于发酵生淀粉材料，而不需要预糖化。

    Enzymatic glyceride hydrolysis
    4.
    发明授权
    Enzymatic glyceride hydrolysis 失效
    酶甘油酯水解

    公开(公告)号：US4394445A

    公开(公告)日：1983-07-19

    申请号：US331449

    申请日：1981-12-15

    申请人： Paul T. Nix ,  Janet M. Santoro ,  Joyce E. Stephens

    发明人： Paul T. Nix ,  Janet M. Santoro ,  Joyce E. Stephens

    IPC分类号： C11B1/04 ,  C12N9/20 ,  C12Q1/44

    CPC分类号： C12R1/39 ,  C11B1/04 ,  C12N9/20 ,  C12Q1/44 ,  C12R1/845

    摘要： A new stable combination of Rhizopus arrhizus lipase and Pseudomonas fluorescens lipase for hydrolyzing triglycerides to free glycerol in body fluids is disclosed. Substantially complete and rapid hydrolysis is provided by a mixture of enzymes using a ratio between 2:1 and 10:1, about 5:1 being preferred, the amounts representing units of activity of each lipase. Approximately 150 units of total lipases will provide substantially complete hydrolysis of the triglycerides contained in 20 microliters of serum up to a minimum concentration of 5 g./l. within 2.5 minutes at 37.degree. C.

    摘要翻译： 公开了根瘤菌脂肪酶和荧光假单胞菌脂肪酶的新稳定组合，用于水解甘油三酯以释放体液中的甘油。 通过使用2:1至10:1之间的比例的酶的混合物提供基本上完全和快速的水解，优选约5:1，表示每种脂肪酶的活性单位的量。 总共约150单位的总脂肪酶将提供20微升血清中含有的甘油三酯的基本上完全水解，最高浓度为5克/升。 在37℃2.5分钟内

    Strains of Rhizopus oryzae and uses thereof
    5.
    发明授权
    Strains of Rhizopus oryzae and uses thereof 有权
    米曲霉菌株及其用途

    公开(公告)号：US06849444B2

    公开(公告)日：2005-02-01

    申请号：US10127679

    申请日：2002-04-23

    申请人： Hungming J. Liaw

    发明人： Hungming J. Liaw

    IPC分类号： C12N1/20 ,  C12P7/56 ,  C12N1/14

    CPC分类号： C12R1/845 ,  C12N1/20 ,  C12P7/56

    摘要： Disclosed are novel strains of Rhizopus oryzae and uses thereof. The strains of the invention are temperature-resistant and convert a carbon source to lactic acid at high temperatures.

    摘要翻译： 公开了新霉菌根霉菌及其用途。 本发明的菌株是耐温的，并且在高温下将碳源转化为乳酸。

    Rhizopus host vector system
    6.
    发明授权
    Rhizopus host vector system 失效
    根霉宿主载体系统

    公开(公告)号：US5436158A

    公开(公告)日：1995-07-25

    申请号：US165881

    申请日：1993-12-14

    申请人： Masamichi Takagi ,  Hiroyuki Horiuchi ,  Koji Yanai ,  Kenji Sakaguchi

    发明人： Masamichi Takagi ,  Hiroyuki Horiuchi ,  Koji Yanai ,  Kenji Sakaguchi

    IPC分类号： C12N1/15 ,  C12N15/09 ,  C12N15/80 ,  C12R1/785 ,  C12R1/845

    CPC分类号： C12N15/80 ,  C12R1/845

    摘要： A host vector system comprising Rhizopus species such as Rhizopus niveus (IFO4810) and a plasmid derived from Phycomycetes such as Mucor, Phycomyces and Absidia wherein the plasmid is contained in a chromosome and/or cytoplasm of the Rhizopus species. The host vector system is suitable for the extracellular production of proteins and enzymes. A process for preparing the host vector system is also provided.

    摘要翻译： 包括根霉属（Rhizopus niveus）（IFO4810）和源自诸如毛霉属，植物分枝杆菌和唾液霉菌属的植物病原体的质粒的宿主载体系统，其中所述质粒包含在根霉属的染色体和/或细胞质中。 宿主载体系统适用于蛋白质和酶的细胞外生成。 还提供了用于制备宿主载体系统的方法。

    ML-236B Derivatives and their preparation
    7.
    发明授权
    ML-236B Derivatives and their preparation 失效
    ML-236B衍生物及其制备方法

    公开(公告)号：US4410629A

    公开(公告)日：1983-10-18

    申请号：US351974

    申请日：1982-02-24

    申请人： Akira Terahara ,  Minoru Tanaka

    发明人： Akira Terahara ,  Minoru Tanaka

    IPC分类号： C07C69/03 ,  C07D309/30 ,  C12P7/62 ,  C12P17/06 ,  C12P7/42

    CPC分类号： C12R1/465 ,  C07D309/30 ,  C12P17/06 ,  C12P7/62 ,  C12R1/645 ,  C12R1/65 ,  C12R1/785 ,  C12R1/845 ,  Y10S435/886

    摘要： Compounds of formula (I): ##STR1## (wherein R represents a group of formula ##STR2## and the corresponding ring-closed lactones, salts (especially alkali metal salts) and esters (especially C.sub.1 -C.sub.5 alkyl esters) thereof may be prepared by subjecting ML-236B, or ML-236B carboxylic acid or a salt or ester thereof to enzymatic hydroxylation, which may be effected by means of microorganisms of the genera Mucor, Rhizopus, Zygorynchus, Circinella, Actinomucor, Gongornella, Phycomyces, Martierella, Pycnoporus, Rhizoctonia, Absidia, Cunninghamella, Syncephalastrum and Streptomyces, or cell-free, enzyme-containing extracts from said microorganisms. The compounds are capable of inhibiting biosynthesis of cholesterol and are thus useful in the treatment of hypercholesteraemia.

    摘要翻译： 式（I）的化合物：其中R表示式（Ⅰ）的基团或相应的闭环内酯，盐（特别是碱金属盐）和酯（特别是C 1 -C 5） 烷基酯）可以通过使ML-236B或ML-236B羧酸或其盐或酯进行酶羟基化来制备，其可以通过毛霉属，根霉属，丝状芽孢杆菌属，圆弧菌属，放线菌属， 金盏草，植物疫霉，火鸡lla，，us，，，Ab ia，，，，，，and and and。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。

    Mutant Filamentous Fungus and Method for Producing C4 Dicarboxylic Acid Using Same
    8.
    发明申请
    Mutant Filamentous Fungus and Method for Producing C4 Dicarboxylic Acid Using Same 审中-公开

    公开(公告)号：US20190194700A1

    公开(公告)日：2019-06-27

    申请号：US16330173

    申请日：2017-09-05

    申请人： Kao Corporation

    发明人： Kyoshiro NONAKA

    IPC分类号： C12P7/46 ,  C12N1/14 ,  C12R1/845

    CPC分类号： C12P7/46 ,  C12N1/14 ,  C12N15/09 ,  C12R1/845

    摘要： Provided is a mutant filamentous fungus having improved C4 dicarboxylic acid productivity and a method for producing a C4 dicarboxylic acid using the mutant filamentous fungus. A mutant filamentous fungus having enhanced expression of at least one polypeptide selected from the group consisting of a polypeptide consisting of the amino acid sequence represented by SEQ ID No: 2; a polypeptide consisting of an amino acid sequence having an identity of at least 90% with the amino acid sequence represented by SEQ ID No: 2 and having malic enzyme activity; and a polypeptide consisting of an amino acid sequence having deletion, substitution, addition or insertion of one or more amino acids with respect to the amino acid sequence represented by SEQ ID No: 2 and having malic enzyme activity.

    Rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of fermenting to produce fumaric acid
    9.
    发明授权
    Rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of fermenting to produce fumaric acid 有权
    米根霉菌株，其诱变和筛选方法以及发酵产生富马酸的方法

    公开(公告)号：US08663972B2

    公开(公告)日：2014-03-04

    申请号：US13992233

    申请日：2011-04-16

    申请人： He Huang ,  Shuang Li ,  Qing Xu ,  Zhen Gao

    发明人： He Huang ,  Shuang Li ,  Qing Xu ,  Zhen Gao

    IPC分类号： C12N1/14 ,  C12N15/01 ,  C12P7/46 ,  C12P19/20

    CPC分类号： C12P7/46 ,  C12N1/14 ,  C12N15/01 ,  C12R1/845

    摘要： A rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named as Rhizopus oryzae ME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying.

    摘要翻译： 根霉菌株，其诱变和筛选方法，以及通过发酵产生富马酸的方法。 该菌株被命名为米曲霉ME-F13，并存放于中国型号培养基中心，存放号码为CCTCC M 2010351.该菌株是通过离子注射物理诱变原始菌株ME-F12获得的， 含有2-D脱氧葡萄糖（2-DG）的固体选择性板和拾取2-DG抗性单个菌落。 该菌株能够同时糖化淀粉物质并发酵以产生富马酸。 具有改善的酶活性，该菌株可以直接用于发酵生淀粉材料，而不需要预糖化。