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公开(公告)号:US07011961B2
公开(公告)日:2006-03-14
申请号:US10257465
申请日:2002-02-14
申请人: Kap-Soo Noh , Yeong-Chul Kim , Jae-Yong Park , Dai-Chul Kim , Jin-Ho Lee , Seung-Han Ok
发明人: Kap-Soo Noh , Yeong-Chul Kim , Jae-Yong Park , Dai-Chul Kim , Jin-Ho Lee , Seung-Han Ok
摘要: A method of producing L-threonine using a microorganism is provided, one or more copies of each of the phosphoenolpyruvate carboxylase gene and the threonine operon are additionally intedgrated into a particular site of the chromosomal DNA of the microorganism, whiel its inherent phophoenolpyruvate carboxylase gene and threonine operon remain.
摘要翻译: 提供了使用微生物生产L-苏氨酸的方法,将一种或多种磷酸烯醇丙酮酸羧化酶基因和苏氨酸操纵子的拷贝额外地加入到微生物的染色体DNA的特定位点,与其固有的磷酸烯醇丙酮酸羧化酶基因和 苏氨酸操纵子依然存在。
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公开(公告)号:US20050136518A1
公开(公告)日:2005-06-23
申请号:US11047932
申请日:2005-02-01
申请人: Kap-Soo Noh , Yeong-Chul Kim , Jae-Yong Park , Dai-Chul Kim , Jin-Ho Lee , Seung-Han Ok
发明人: Kap-Soo Noh , Yeong-Chul Kim , Jae-Yong Park , Dai-Chul Kim , Jin-Ho Lee , Seung-Han Ok
摘要: A method for producing L-threonine using a microorganism is provided. In the method, additional one or more copies of each of the phosphoenolpyruvate carboxylase (ppc) gene and the threonine operon are integrated into a particular site of the chromosomal DNA of a microorganism, while its inherent ppc gene and threonine operon remain. Accordingly, two or more ppc genes and threonine operons are included in the chromosomal DNA of the microorganism to thereby enhance the expression of the ppc gene encoding an enzyme to convert phosphoenolpyruvate to a threonine biosynthesis precursor, oxaloacetete, and the genes encoding enzymes involved in the synthetic pathway of threonine from oxaloacetate, including thrA (aspartokinasel-homoserine dehydrogenase), thrB (homoserine kinase), and thrC (threonine synthase), thereby markedly increasing L-threonine productivity.
摘要翻译: 提供了使用微生物生产L-苏氨酸的方法。 在该方法中,每个磷酸烯醇丙酮酸羧化酶(ppc)基因和苏氨酸操纵子的另外一个或多个拷贝整合到微生物的染色体DNA的特定位点,而其固有的ppc基因和苏氨酸操纵子保留。 因此,在微生物的染色体DNA中包含两个以上的ppc基因和苏氨酸操纵子,从而增强编码酶的ppc基因的表达,以将磷酸烯醇丙酮酸转化为苏氨酸生物合成前体,oxaloacetete,以及编码参与 苏氨酸从草酰乙酸(包括thrA(天冬氨酸高丝氨酸脱氢酶)),thrB(高丝氨酸激酶)和thrC(苏氨酸合成酶)的合成途径,从而显着提高L-苏氨酸生产力。
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公开(公告)号:US07368266B2
公开(公告)日:2008-05-06
申请号:US11047932
申请日:2005-02-01
申请人: Kap-Soo Noh , Yeong-Chul Kim , Jae-Yong Park , Dai-Chul Kim , Jin-Ho Lee , Seung-Han Ok
发明人: Kap-Soo Noh , Yeong-Chul Kim , Jae-Yong Park , Dai-Chul Kim , Jin-Ho Lee , Seung-Han Ok
摘要: A method for producing L-threonine using a microorganism is provided. In the method, additional one or more copies of each of the phosphoenolpyruvate carboxylase (ppc) gene and the threonine operon are integrated into a particular site of the chromosomal DNA of a microorganism, while its inherent ppc gene and threonine operon remain. Accordingly, two or more ppc genes and threonine operons are included in the chromosomal DNA of the microorganism to thereby enhance the expression of the ppc gene encoding an enzyme to convert phosphoenolpyruvate to a threonine biosynthesis precursor, oxaloacetete, and the genes encoding enzymes involved in the synthetic pathway of threonine from oxaloacetate, including thrA (aspartokinasel-homoserine dehydrogenase), thrB (homoserine kinase), and thrC (threonine synthase), thereby markedly increasing L-threonine productivity.
摘要翻译: 提供了使用微生物生产L-苏氨酸的方法。 在该方法中,每个磷酸烯醇丙酮酸羧化酶(ppc)基因和苏氨酸操纵子的另外一个或多个拷贝整合到微生物的染色体DNA的特定位点,而其固有的ppc基因和苏氨酸操纵子保留。 因此,在微生物的染色体DNA中包含两个以上的ppc基因和苏氨酸操纵子,从而增强编码酶的ppc基因的表达,以将磷酸烯醇丙酮酸转化为苏氨酸生物合成前体,oxaloacetete,以及编码参与 苏氨酸从草酰乙酸(包括thrA(天冬氨酸高丝氨酸脱氢酶)),thrB(高丝氨酸激酶)和thrC(苏氨酸合成酶)的合成途径,从而显着提高L-苏氨酸生产力。
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