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    Composition and method for enabling proliferation of pluripotent stem cells
    1.
    发明申请
    Composition and method for enabling proliferation of pluripotent stem cells 有权
    使多能干细胞增殖的组成和方法

    公开(公告)号：US20100323443A1

    公开(公告)日：2010-12-23

    申请号：US12583750

    申请日：2009-08-25

    申请人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

    发明人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0606 ,  C12N2533/52

    摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.

    摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，其既维持干细胞体外多能性并且实现自我更新。 在这方面，已经发现，当在不存在分化抑制剂或饲养细胞的情况下，将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（层粘连蛋白-51）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

    Composition and method for enabling proliferation of pluripotent stem cells
    3.
    发明授权
    Composition and method for enabling proliferation of pluripotent stem cells 有权
    使多能干细胞增殖的组成和方法

    公开(公告)号：US08722405B2

    公开(公告)日：2014-05-13

    申请号：US11969620

    申请日：2008-01-04

    申请人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

    发明人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/07 ,  C12N5/10

    CPC分类号： C12N5/0606 ,  C12N2533/52

    摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, for culturing stem cells in vitro in an undifferentiated state. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or laminin-5 (laminin-322), or their functional domains, the embryonic stem cells proliferated and maintained their pluripotency.

    摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，用于在未分化状态下体外培养干细胞。 在这方面，已经发现，当将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（层粘连蛋白-511）或层粘连蛋白-5（层粘连蛋白-322）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

    Composition and method for enabling proliferation of pluripotent stem cells
    5.
    发明授权
    Composition and method for enabling proliferation of pluripotent stem cells 有权
    使多能干细胞增殖的组成和方法

    公开(公告)号：US08951799B2

    公开(公告)日：2015-02-10

    申请号：US12583750

    申请日：2009-08-25

    申请人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

    发明人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/10 ,  C12N5/071 ,  C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N2533/52

    摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.

    摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，其既维持干细胞体外多能性并且实现自我更新。 在这方面，已经发现，当在不存在分化抑制剂或饲养细胞的情况下，将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（laminin-511）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

    COMPOSITION AND METHOD FOR ENABLING PROLIFERATION OF PLURIPOTENT STEM CELLS
    6.
    发明申请
    COMPOSITION AND METHOD FOR ENABLING PROLIFERATION OF PLURIPOTENT STEM CELLS 有权
    用于促进肺纤维化细胞增殖的组合物和方法

    公开(公告)号：US20080213885A1

    公开(公告)日：2008-09-04

    申请号：US11969620

    申请日：2008-01-04

    申请人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

    发明人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0606 ,  C12N2533/52

    摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, for culturing stem cells in vitro in an undifferentiated state. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or laminin-5 (laminin-322), or their functional domains, the embryonic stem cells proliferated and maintained their pluripotency.

    摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，用于在未分化状态下体外培养干细胞。 在这方面，已经发现，当将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（层粘连蛋白-511）或层粘连蛋白-5（层粘连蛋白-322）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

    Maintenance of differentiated cells with laminins
    7.
    发明授权
    Maintenance of differentiated cells with laminins 有权
    维持层粘连蛋白分化细胞

    公开(公告)号：US09499794B2

    公开(公告)日：2016-11-22

    申请号：US13866177

    申请日：2013-04-19

    申请人： Karl Tryggvason ,  Karl Kristian Tryggvason ,  Anna Domogatskaya

    发明人： Karl Tryggvason ,  Karl Kristian Tryggvason ,  Anna Domogatskaya

    IPC分类号： C12N5/071 ,  C12N5/00

    CPC分类号： C12N5/0068 ,  C12N5/0676 ,  C12N2501/998 ,  C12N2533/52

    摘要： The present disclosure describes methods of maintaining the phenotype of differentiated cells. Generally, the natural environment of the body is replicated for the differentiated cell. The differentiated cell is plated on a cell culture substrate comprising a laminin, such as laminin-521 or laminin-511. The substrate may also contain a cadherin. This maintains the phenotype of the differentiated cell.

    摘要翻译： 本公开描述了维持分化细胞表型的方法。 通常，体内的天然环境被复制用于分化细胞。 将分化的细胞接种在包含层粘连蛋白如层粘连蛋白-51或层粘连蛋白-511的细胞培养基质上。 底物也可以含有钙粘着素。 这维持了分化细胞的表型。

    MAINTENANCE OF DIFFERENTIATED CELLS WITH LAMININS
    8.
    发明申请
    MAINTENANCE OF DIFFERENTIATED CELLS WITH LAMININS 有权
    维持细胞与层间粘附

    公开(公告)号：US20130280750A1

    公开(公告)日：2013-10-24

    申请号：US13866177

    申请日：2013-04-19

    申请人： Karl Tryggvason ,  Karl Kristian Tryggvason ,  Anna Domogatskaya

    发明人： Karl Tryggvason ,  Karl Kristian Tryggvason ,  Anna Domogatskaya

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N5/0676 ,  C12N2501/998 ,  C12N2533/52

    摘要： The present disclosure describes methods of maintaining the phenotype of differentiated cells. Generally, the natural environment of the body is replicated for the differentiated cell. The differentiated cell is plated on a cell culture substrate comprising a laminin, such as laminin-521 or laminin-511. The substrate may also contain a cadherin. This maintains the phenotype of the differentiated cell.

    摘要翻译： 本公开描述了维持分化细胞表型的方法。 通常，体内的天然环境被复制用于分化细胞。 将分化的细胞接种在包含层粘连蛋白如层粘连蛋白-51或层粘连蛋白-511的细胞培养基质上。 底物也可以含有钙粘着素。 这维持了分化细胞的表型。

    Cell culture medium
    9.
    发明授权
    Cell culture medium 有权

    公开(公告)号：US10138292B2

    公开(公告)日：2018-11-27

    申请号：US13328156

    申请日：2011-12-16

    申请人： Karl Tryggvason ,  Sergey Rodin

    发明人： Karl Tryggvason ,  Sergey Rodin

    IPC分类号： C07K14/78 ,  C12N5/0735 ,  C12N5/074

    摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.