公开(公告)号：US20100323443A1

公开(公告)日：2010-12-23

申请号：US12583750

申请日：2009-08-25

申请人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

发明人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

IPC分类号： C12N5/00

CPC分类号： C12N5/0606 ,  C12N2533/52

摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.

摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，其既维持干细胞体外多能性并且实现自我更新。 在这方面，已经发现，当在不存在分化抑制剂或饲养细胞的情况下，将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（层粘连蛋白-51）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

公开(公告)号：US20100203635A1

公开(公告)日：2010-08-12

申请号：US12725877

申请日：2010-03-17

申请人： Karl Tryggvason ,  Sergey Rodin ,  Anna Domogatskaya

发明人： Karl Tryggvason ,  Sergey Rodin ,  Anna Domogatskaya

IPC分类号： C12N5/07

CPC分类号： C12N5/0606 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/998 ,  C12N2533/52

摘要： Compositions and processes for culturing human stem cells in vitro in an undifferentiated state are disclosed. In this regard, human embryonic stem cells proliferated and maintained their pluripotency when cultured on plates coated with recombinant laminin-10 (laminin-511).

摘要翻译： 公开了以未分化状态体外培养人类干细胞的组合物和方法。 在这方面，人胚胎干细胞在用重组层粘连蛋白-10（层粘连蛋白-511）包被的平板上培养时增殖并维持其多能性。

公开(公告)号：US08722405B2

公开(公告)日：2014-05-13

申请号：US11969620

申请日：2008-01-04

申请人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

发明人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/07 ,  C12N5/10

CPC分类号： C12N5/0606 ,  C12N2533/52

摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, for culturing stem cells in vitro in an undifferentiated state. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or laminin-5 (laminin-322), or their functional domains, the embryonic stem cells proliferated and maintained their pluripotency.

摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，用于在未分化状态下体外培养干细胞。 在这方面，已经发现，当将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（层粘连蛋白-511）或层粘连蛋白-5（层粘连蛋白-322）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

公开(公告)号：US10829733B2

公开(公告)日：2020-11-10

申请号：US12725877

申请日：2010-03-17

申请人： Karl Tryggvason ,  Sergey Rodin ,  Anna Domogatskaya

发明人： Karl Tryggvason ,  Sergey Rodin ,  Anna Domogatskaya

IPC分类号： C12N5/0735

摘要： Compositions and processes for culturing human stem cells in vitro in an undifferentiated state are disclosed. In this regard, human embryonic stem cells proliferated and maintained their pluripotency when cultured on plates coated with recombinant laminin-10 (laminin-511).

公开(公告)号：US08951799B2

公开(公告)日：2015-02-10

申请号：US12583750

申请日：2009-08-25

申请人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

发明人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/10 ,  C12N5/071 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12N2533/52

摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.

摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，其既维持干细胞体外多能性并且实现自我更新。 在这方面，已经发现，当在不存在分化抑制剂或饲养细胞的情况下，将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（laminin-511）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

公开(公告)号：US20080213885A1

公开(公告)日：2008-09-04

申请号：US11969620

申请日：2008-01-04

申请人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

发明人： Karl Tryggvason ,  Anna Domogatskaya ,  Sergey Rodin

IPC分类号： C12N5/06

CPC分类号： C12N5/0606 ,  C12N2533/52

摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, for culturing stem cells in vitro in an undifferentiated state. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or laminin-5 (laminin-322), or their functional domains, the embryonic stem cells proliferated and maintained their pluripotency.

摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，用于在未分化状态下体外培养干细胞。 在这方面，已经发现，当将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（层粘连蛋白-511）或层粘连蛋白-5（层粘连蛋白-322）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

公开(公告)号：US10138292B2

公开(公告)日：2018-11-27

申请号：US13328156

申请日：2011-12-16

申请人： Karl Tryggvason ,  Sergey Rodin

发明人： Karl Tryggvason ,  Sergey Rodin

IPC分类号： C07K14/78 ,  C12N5/0735 ,  C12N5/074

摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.

公开(公告)号：US09534038B2

公开(公告)日：2017-01-03

申请号：US13409594

申请日：2012-03-01

申请人： Karl Tryggvason ,  Sergey Rodin

发明人： Karl Tryggvason ,  Sergey Rodin

IPC分类号： C12N15/12 ,  A61K38/39 ,  C07K14/78 ,  A61K38/00

CPC分类号： C07K14/78 ,  A61K38/00 ,  C07K2319/033 ,  C07K2319/036 ,  C07K2319/09 ,  C07K2319/41 ,  C07K2319/43

摘要： The present disclosure provides isolated laminin-421, methods for making recombinant laminin-421, and host cells that express recombinant laminin-421. The present disclosure also provides nucleic acid sequences encoding full length human laminin β2 chain, expression vectors and host cells thereof.

摘要翻译： 本公开提供分离的层粘连蛋白421，用于制备重组层粘连蛋白421的方法和表达重组层粘连蛋白421的宿主细胞。 本公开还提供了编码全长人层粘连蛋白β2链，表达载体和宿主细胞的核酸序列。

公开(公告)号：US08415156B2

公开(公告)日：2013-04-09

申请号：US13328145

申请日：2011-12-16

申请人： Karl Tryggvason ,  Sergey Rodin

发明人： Karl Tryggvason ,  Sergey Rodin

IPC分类号： C12N5/00 ,  A01N63/00

CPC分类号： C07K14/78 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2510/00 ,  C12N2533/52 ,  Y10T428/13

摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with a matrix of recombinant laminin-521 (laminin 11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.

摘要翻译： 本公开涉及分离的层粘连蛋白-521，制备重组层粘连蛋白521的方法，表达重组层粘连蛋白-521的宿主细胞和含有层粘连蛋白-51的组合物。 层粘连蛋白-521可以维持干细胞的体外多能性，实现自我更新，并能使人类胚胎干细胞的单细胞存活。 当多能人胚胎干细胞在涂有重组层粘连蛋白-51（层粘连蛋白11）基质的平板上培养时，在不存在分化抑制剂或饲养细胞的情况下，胚胎干细胞增殖并保持其多能性。 还已经发现人重组层粘连蛋白-51（层粘连蛋白-11）在完全解离成单细胞悬液后提供干细胞的单细胞存活。 含有至多3.9ng / ml的β成纤维细胞生长因子（bFGF）的有用细胞培养基也在本文中描述。

公开(公告)号：US20120302512A1

公开(公告)日：2012-11-29

申请号：US13409594

申请日：2012-03-01

申请人： Karl Tryggvason ,  Sergey Rodin

发明人： Karl Tryggvason ,  Sergey Rodin

IPC分类号： C07K14/435 ,  C12P21/02 ,  A61K38/14

CPC分类号： C07K14/78 ,  A61K38/00 ,  C07K2319/033 ,  C07K2319/036 ,  C07K2319/09 ,  C07K2319/41 ,  C07K2319/43

摘要： The present disclosure provides isolated laminin-421, methods for making recombinant laminin-421, and host cells that express recombinant laminin-421. The present disclosure also provides nucleic acid sequences encoding full length human laminin β2 chain, expression vectors and host cells thereof.

摘要翻译： 本公开提供分离的层粘连蛋白421，用于制备重组层粘连蛋白421的方法和表达重组层粘连蛋白421的宿主细胞。 本公开还提供了编码全长人层粘连蛋白和其2链，表达载体和宿主细胞的核酸序列。