Method for the preparation of k-carrageenase
    5.
    发明申请
    Method for the preparation of k-carrageenase 有权
    制备k-角叉菜胶酶的方法

    公开(公告)号:US20070231866A1

    公开(公告)日:2007-10-04

    申请号:US11509755

    申请日:2006-08-25

    IPC分类号: C12P19/04 C12N1/20

    CPC分类号: C12N9/2402 C12Y302/01083

    摘要: Halophilic marine gram negative bacteria, isolated from decayed algae, hydrolyzed κ-carrageenan. To maximize κ-carrageenase production in the cost effective manner, a novel medium was defined having minimum components and their optimum concentration by statistical optimization method. Hence there evolved a novel medium composition for enhanced κ-carrageenase production by a new halotolerant marine bacterium, Pseudomonas spp.

    摘要翻译: 嗜盐海洋革兰氏阴性细菌,从腐烂藻类中分离出来,水解卡拉卡拉胶。 为了以成本有效的方式最大化卡帕卡拉胶酶生产,通过统计优化方法定义了具有最小成分和最佳浓度的新型培养基。 因此,通过新的耐盐海洋细菌假单胞菌属(Pseudomonas spp。)发展了用于增强κ-角叉菜胶酶生产的新型培养基组合物。

    Solid nutrient media useful for isolating and identifying alkaliphilic bacteria
    6.
    发明授权
    Solid nutrient media useful for isolating and identifying alkaliphilic bacteria 有权
    固体营养培养基可用于分离和鉴定嗜碱性细菌

    公开(公告)号:US08097447B2

    公开(公告)日:2012-01-17

    申请号:US12240043

    申请日:2008-09-29

    IPC分类号: C12N1/20 C12Q1/04 C12Q1/00

    CPC分类号: C12N1/20 C12Q1/045

    摘要: The present invention relates to a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form. The media composition consists of 5-15 g of carbon source, 2.5-10 g of peptone, 2.5-10 g of yeast extract, 0.5-1.5 g of dipotassium hydrogen phosphate; 0.1-0.5 g of magnesium sulphate heptahydrate, 30 μl-4 ml of super saturated solution of sodium hydroxide, 5-20 g of potassium chloride and 10-30 g of κ-carrageenan in one liter of distilled water. The potassium salt in combination with κ-carrageenan in specific proportion has been found to be a suitable replacement of agar in solidifying bacteriological media especially, for isolation of extreme alkaliphiles. The present invention also provides a method of using the solid nutrient media composition having alkaline pH to study biodiversity of cultivable alkaliphilic bacteria.

    摘要翻译: 本发明涉及具有碱性pH的固体营养培养基组合物,其用于分离和鉴定纯形式的嗜碱性微生物。 培养基组成由5-15g碳源,2.5-10g蛋白胨,2.5-10g酵母提取物,0.5-1.5g磷酸氢二钾组成; 0.1-0.5g七水合硫酸镁,30μl-4ml超饱和氢氧化钠溶液,5-20g氯化钾和10-30g&角叉菜胶在1升蒸馏水中的溶液。 已经发现与特定比例的钾盐与卡拉胶的组合是在固化细菌培养基中的合适的琼脂替代物,特别是用于分离极碱性碱性物质。 本发明还提供了使用具有碱性pH的固体营养培养基组合物来研究可培养的嗜碱细菌的生物多样性的方法。

    Method for the preparation of κ-carrageenase
    7.
    发明授权
    Method for the preparation of κ-carrageenase 有权
    卡拉胶酶的制备方法

    公开(公告)号:US07951561B2

    公开(公告)日:2011-05-31

    申请号:US11509755

    申请日:2006-08-25

    IPC分类号: C12P21/04

    CPC分类号: C12N9/2402 C12Y302/01083

    摘要: Halophilic marine gram negative bacteria, isolated from decayed algae, hydrolyzed κ-carrageenan. To maximize κ-carrageenase production in the cost effective manner, a novel medium was defined having minimum components and their optimum concentration by statistical optimization method. Hence there evolved a novel medium composition for enhanced κ-carrageenase production by a new halotolerant marine bacterium, Pseudomonas spp.

    摘要翻译: 嗜盐海洋革兰氏阴性细菌,从腐烂藻类中分离出来,水解和角叉菜胶。 为了以成本有效的方式最大化和克拉霉素生产,通过统计优化方法定义了具有最小成分和最佳浓度的新型培养基。 因此,通过新的耐盐性海洋细菌,假单胞菌属(Pseudomonas spp。)发展出一种用于增强和克拉霉素生产的新型培养基组合物。