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公开(公告)号：US08604184B2
公开(公告)日：2013-12-10
申请号：US12774194
申请日：2010-05-05
CPC分类号： A61K31/7088 , Y02A50/471 , Y02A50/473
摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound are provided. The present invention is directed to an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target. The second binding sites are preferably thiolated aptamers that have the benefit of increased stability, resistance to degradation and longer circulating half life. Methods of making and using pharmaceutical compositions including immunity linker molecules having a thiolated aptamer are also provided.
摘要翻译： 提供了立即将个体免疫任何分子或化合物的方法和组合物。 本发明涉及具有至少两个位点的免疫接头; （1）结合个体中的免疫应答组分的至少一个第一结合位点，和（2）至少一个第二结合位点，其特异性结合所需化合物或分子，靶标。 第二结合位点优选为具有增加的稳定性，耐降解性和更长的循环半衰期益处的硫醇化适体。 还提供了制备和使用药物组合物的方法，包括具有巯基适体的免疫接头分子。
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公开(公告)号：US09217134B1
公开(公告)日：2015-12-22
申请号：US14470506
申请日：2014-08-27
IPC分类号： C12N15/115 , C12Q1/68 , C12N1/20 , G01N33/50
CPC分类号： G01N33/56938 , C12N15/115 , C12N2310/16
摘要： Aptamers and methods of use thereof are presented for the treatment or diagnosis of Staphylococcus aureus infection. The aptamers binds to and capable of neutralization of Staphylococcus aureus alpha-toxin.
摘要翻译： 提供了一种用于治疗或诊断金黄色葡萄球菌感染的用途及其使用方法。 适体与金黄色葡萄球菌α-毒素结合并能够中和。
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公开(公告)号：US20120134877A1
公开(公告)日：2012-05-31
申请号：US12973681
申请日：2010-12-20
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
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公开(公告)号：US06569630B1
公开(公告)日：2003-05-27
申请号：US09978753
申请日：2001-10-15
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods of preparing nucleic acid ligands against anthrax spores, compositions comprising anthrax specific nucleic acid ligands and methods of use of such ligands for detection and/or neutralization of anthrax spores.
摘要翻译： 本发明涉及制备针对炭疽孢子的核酸配体的方法，包含炭疽特异性核酸配体的组合物和用于检测和/或中和炭疽孢子的这种配体的使用方法。
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公开(公告)号：US08383036B2
公开(公告)日：2013-02-26
申请号：US12973681
申请日：2010-12-20
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
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6.发明授权公开(公告)号：US07892484B2
公开(公告)日：2011-02-22
申请号：US10291336
申请日：2002-11-08
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译： 本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。 所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。 尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。 示例性有机半导体包括DAT和DALM。 在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。 优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。 其他实施例涉及包括有机半导体和能量源的装置。 在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。
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公开(公告)号：US08318438B2
公开(公告)日：2012-11-27
申请号：US12072758
申请日：2008-02-27
IPC分类号： C12Q1/00 , C12Q1/68 , G01N33/00 , G01N33/554 , G01N33/569
CPC分类号： G01N33/5308 , G01N2333/195
摘要： We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.
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公开(公告)号：US20120157670A1
公开(公告)日：2012-06-21
申请号：US12072758
申请日：2008-02-27
IPC分类号： C07H21/04 , G01N33/569 , G01N33/53
CPC分类号： G01N33/5308 , G01N2333/195
摘要： We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.
摘要翻译： 我们描述使用适配体捕获和报告目标（例如病原体）的存在的实例。 这里描述的实例包括一组特异于土拉氏木霉的适体。 本文描述的其它实例包括Aptamer-Linked固定吸附测定（ALISA）和斑点印迹测定。 本文提供的方法的实例包括：提供一组对靶抗原具有高结合亲和力的DNA序列，将DNA序列置于夹心适体连接的固定化吸附剂测定（ALISA）中，使DNA序列与样品接触，以及 检测目标是否存在于样本中。 一些替代实现可以包括点印迹和不同的记者。 可以使用量子点夹心测定法和量子点去猝灭记录仪。
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