公开(公告)号：US20060172419A1

公开(公告)日：2006-08-03

申请号：US10539534

申请日：2003-12-24

申请人： Kazuaki Umeda ,  Takeshi Matsui ,  Mayumi Nakayama

发明人： Kazuaki Umeda ,  Takeshi Matsui ,  Mayumi Nakayama

IPC分类号： A01K67/027 ,  C12N5/06 ,  C12N15/87

CPC分类号： C07K14/47 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/105 ,  C12N15/8509 ,  C12N15/907 ,  C12N2800/30 ,  C12N2830/42 ,  C12N2840/203 ,  C12N2840/44

摘要： In the process of generating ZO-1 gene knockout mice, the present inventors developed gene targeting vectors that allow homologous recombination to occur at a high probability (90% or more). In addition, the present inventors used the targeting vectors for studying optimal electroporation conditions in epithelial cells, and as a result, successfully determined optimal electroporation conditions that would enable efficient gene targeting in the EpH4 mouse epithelial cell line. Use of the vectors of the present invention enables easy introduction of exogenous genes into the ZO-1 allele of ES cells. Moreover, since effects on the genomic structure need not be considered, the methods of the present invention are expected to solve problems encountered in the conventional methods for generating transgenic mice and stable transformants.

摘要翻译： 在产生ZO-1基因敲除小鼠的过程中，本发明人开发了以高概率（90％或更高）发生同源重组的基因靶向载体。 此外，本发明人使用靶向载体来研究上皮细胞中的最佳电穿孔条件，结果成功地确定了能够在EpH4小鼠上皮细胞系中实现有效基因靶向的最佳电穿孔条件。 使用本发明的载体能够容易地将外源基因引入到ES细胞的ZO-1等位基因中。 此外，由于不需要考虑对基因组结构的影响，预期本发明的方法可以解决用于产生转基因小鼠和稳定转化体的常规方法中遇到的问题。

公开(公告)号：US08879721B2

公开(公告)日：2014-11-04

申请号：US11680665

申请日：2007-03-01

申请人： Takeshi Matsui

发明人： Takeshi Matsui

IPC分类号： H04M9/08

CPC分类号： H04M9/082

摘要： An audio communication system includes a first analog/digital convertor, a second analog/digital convertor, and an echo canceller. The first analog/digital convertor converts an audio signal, which is input from a microphone, to a data signal. The second analog/digital convertor that converts an audio signal, which is input from an auxiliary input terminal, to a data signal. When an audio signal transmitted from the outside is received and output from a speaker, the echo canceller prevents an output of the speaker from being input through the microphone and echoed back to a recipient or remote device. An operating frequency of the echo canceller is relatively lower than a sampling frequency of the second analog/digital convertor.

摘要翻译： 音频通信系统包括第一模拟/数字转换器，第二模拟/数字转换器和回声消除器。 第一模拟/数字转换器将从麦克风输入的音频信号转换成数据信号。 将从辅助输入端子输入的音频信号转换为数据信号的第二模拟/数字转换器。 当从扬声器接收并输出从外部发送的音频信号时，回声消除器防止扬声器的输出通过麦克风输入并回送到接收者或远程设备。 回波消除器的工作频率相对低于第二模拟/数字转换器的采样频率。

公开(公告)号：US08061792B2

公开(公告)日：2011-11-22

申请号：US12396812

申请日：2009-03-03

申请人： Tatsumi Ito ,  Yuichiro Ikemoto ,  Takeshi Matsui ,  Koji Ashizaki

发明人： Tatsumi Ito ,  Yuichiro Ikemoto ,  Takeshi Matsui ,  Koji Ashizaki

IPC分类号： B41J3/00 ,  B41J2/15

CPC分类号： B41J2/17509 ,  B41J3/4071 ,  B41J29/38

摘要： A printing apparatus includes a printing unit for printing visual information on a non-recording surface of a rotatably driven recording medium by discharging ink droplets. A control unit controls movement of the printing unit in a radial direction of the recording medium, and discharge timing of the ink droplets. The printing unit prints the visual information by multi-path printing by printing on a same radius position of the non-recording surface by discharging ink from different ink discharge nozzles. The control unit controls the printing unit so as to print with a first printing pattern having a first number of paths and a second printing pattern having a second number of paths. The first printing pattern is used on other locations of the non-recording surface and the second printing pattern is used when printing on an innermost periphery region of the non-recording surface.

摘要翻译： 一种打印装置，包括一个打印单元，用于通过排放墨滴在可旋转驱动的记录介质的非记录表面上打印视觉信息。 控制单元控制打印单元沿记录介质的径向的移动，以及墨滴的排出定时。 印刷单元通过在不记录表面的相同半径位置上通过从不同的喷墨喷嘴排出墨来通过多路径打印来打印视觉信息。 控制单元控制打印单元，以便以具有第一数目的路径的第一印刷图案和具有第二数量的路径的第二印刷图案进行打印。 第一印刷图案用于非记录表面的其它位置，并且当在非记录表面的最内周边区域上打印时使用第二印刷图案。

公开(公告)号：US20110231960A1

公开(公告)日：2011-09-22

申请号：US12990597

申请日：2009-04-28

申请人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui

发明人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui

IPC分类号： A01H5/00 ,  C12N15/63 ,  C07K19/00 ,  C07H21/04 ,  C12N5/10 ,  A01H5/10 ,  C07K7/08 ,  C07K14/00

CPC分类号： C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K2039/517 ,  C07K14/25 ,  C07K14/28 ,  C07K2319/00 ,  C07K2319/55 ,  C12N15/8258 ,  Y02A50/472 ,  Y02A50/474

摘要： A bacterial toxin protein such as a Shiga toxin protein is efficiently produced using plant cells. The plant cells are transformed using a DNA construct containing DNA encoding a hybrid protein in which the bacterial toxin proteins such as the Shiga toxin proteins are tandemly linked through a peptide having the following characteristics (A) and (B) to produce the bacterial toxin protein in the plant cells: (A) a number of amino acids is 12 to 30; and (B) a content of proline is 20 to 35%.

摘要翻译： 使用植物细胞有效地产生细菌毒素蛋白如志贺毒素蛋白质。 使用含有编码杂合蛋白的DNA的DNA构建体转化植物细胞，其中细菌毒素蛋白如志贺毒素蛋白通过具有以下特征（A）和（B）的肽串联连接以产生细菌毒素蛋白 在植物细胞中：（A）一些氨基酸为12〜30; （B）脯氨酸含量为20〜35％。

公开(公告)号：US08020952B2

公开(公告)日：2011-09-20

申请号：US12404758

申请日：2009-03-16

申请人： Tatsumi Ito ,  Takeshi Matsui ,  Yuichiro Ikemoto ,  Koji Ashizaki

发明人： Tatsumi Ito ,  Takeshi Matsui ,  Yuichiro Ikemoto ,  Koji Ashizaki

IPC分类号： B41J3/00 ,  B41J29/38 ,  B41J3/407

CPC分类号： B41J3/4071 ,  B41J2/17509 ,  B41J29/38

摘要： There is provided a printing apparatus including a printing unit for printing visual information on a non-recording surface of a rotatably driven recording medium by discharging ink droplets; and a control unit for controlling movement of the printing unit in a radial direction of the rotatably driven recording medium, and controlling a discharge timing of the ink droplets discharged from the printing unit, wherein the printing unit includes a plurality of ink droplet discharge nozzles, arrayed in a line in the radial direction of the recording medium, for discharging ink droplets of different colors by the control of the control unit, and the control unit completes the printing of the visual information by reciprocating the ink droplet discharge nozzles for plural times in the radial direction of the rotatably driven recording medium.

摘要翻译： 提供了一种打印装置，其包括：通过排出墨滴在可旋转驱动的记录介质的非记录表面上打印视觉信息的打印单元; 以及控制单元，用于控制打印单元沿可旋转驱动的记录介质的径向的移动，并且控制从打印单元排出的墨滴的排出正时，其中打印单元包括多个墨滴排出喷嘴， 排列在记录介质的径向上的一行中，用于通过控制单元的控制排出不同颜色的墨滴，并且控制单元通过使墨滴排放喷嘴多次往复运动来完成视觉信息的打印 可旋转驱动的记录介质的径向。

公开(公告)号：US20110002950A1

公开(公告)日：2011-01-06

申请号：US12667393

申请日：2008-03-25

申请人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui ,  Sou-ichi Makino ,  Keiko Kawamoto

发明人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui ,  Sou-ichi Makino ,  Keiko Kawamoto

IPC分类号： A61K39/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8258 ,  A61K39/0258 ,  A61K2039/517 ,  A61K2039/552 ,  C12N15/8257 ,  Y02A50/474

摘要： A technology for producing a pig edema disease vaccine at low cost and at high efficiency is developed. Specifically, a gene of a pig edema disease toxin protein (Stx2e protein) is efficiently expressed in plant cells to produce a plant vaccine for pig edema disease at low cost. An Stx2e protein including a secretory signal peptide derived from a plant added at an amino terminus is expressed in cells of a plant such as Lactuca sativa using the 5′-untranslated region of an alcohol dehydrogenase gene (ADH5′UTR) derived from a plant.

摘要翻译： 开发了一种以低成本，高效率生产猪水肿病疫苗的技术。 具体地说，猪水肿病毒素蛋白（Stx2e蛋白）的基因在植物细胞中有效表达，以低成本生产猪水肿病植物疫苗。 使用源于植物的醇脱氢酶基因（ADH5'UTR）的5'-非翻译区，在植物如莴苣植物的细胞中表达包含衍生自在氨基末端添加的植物的分泌信号肽的Stx2e蛋白质。

公开(公告)号：US07843478B2

公开(公告)日：2010-11-30

申请号：US12009274

申请日：2008-01-17

申请人： Takeshi Matsui

发明人： Takeshi Matsui

IPC分类号： B41J2/435

CPC分类号： B41J3/4071

摘要： A recording medium drive apparatus is disclosed. The recording medium drive apparatus includes a holding mechanism, a data recording means, and a printing means. The holding mechanism holds a recording medium having a print target surface targeted for printing and an optical recording surface for data. The data recording means is capable of recording the data on the optical recording surface held by the holding mechanism. The printing means is capable of performing printing on the print target surface of the recording medium and applying a correction fluid on the print target surface.

摘要翻译： 公开了一种记录介质驱动装置。 记录介质驱动装置包括保持机构，数据记录装置和打印装置。 保持机构保持具有用于打印的打印目标表面和用于数据的光学记录表面的记录介质。 数据记录装置能够将数据记录在由保持机构保持的光学记录表面上。 打印装置能够在记录介质的打印目标表面上执行打印并在打印目标表面上施加校正流体。

公开(公告)号：US20100228381A1

公开(公告)日：2010-09-09

申请号：US12715120

申请日：2010-03-01

申请人： Takeshi Matsui ,  Junichi Kuzusako ,  Hiroyuki Yasukochi

发明人： Takeshi Matsui ,  Junichi Kuzusako ,  Hiroyuki Yasukochi

IPC分类号： G06F17/00

CPC分类号： B29C64/165

摘要： A three-dimensional modeling apparatus includes a stage, a supply mechanism, a head, a movement mechanism, and a lifting and lowering mechanism. On the stage, a powder material is accumulated. The supply mechanism supplies the powder material on the stage for each predetermined layer thickness. The head ejects a liquid for forming a three-dimensional object to the powder material on the stage. The liquid is capable of binding the powder material. The movement mechanism moves the stage so that the liquid is supplied from the head to the powder material by the predetermined layer thickness. The lifting and lowering mechanism lowers the stage for each predetermined layer thickness.

摘要翻译： 三维建模装置包括台架，供给机构，头部，移动机构以及升降机构。 在舞台上堆积粉末材料。 供给机构为每个预定层厚度在粉台上提供粉末材料。 头部将用于形成三维物体的液体喷射到舞台上的粉末材料上。 液体能够结合粉末材料。 移动机构移动台，使得液体从头部向粉末材料供给预定的层厚度。 提升和降低机构降低了每个预定层厚度的阶段。

公开(公告)号：US20090183188A1

公开(公告)日：2009-07-16

申请号：US12351949

申请日：2009-01-12

申请人： Koji Ashizaki ,  Yuichiro Ikemoto ,  Seiji Kobayashi ,  Takeshi Matsui ,  Tatsumi Ito ,  Shintaro Tanaka

发明人： Koji Ashizaki ,  Yuichiro Ikemoto ,  Seiji Kobayashi ,  Takeshi Matsui ,  Tatsumi Ito ,  Shintaro Tanaka

IPC分类号： G11B17/04

CPC分类号： G11B17/056

摘要： A disc recording and/or reproducing apparatus includes a disc mounting unit to which a disc recording medium is removably mounted, the disc mounting unit rotating the disc recording medium; a print head which discharges an ink drop towards a label surface of the disc recording medium that is rotationally driven by the disc mounting unit; and a mist attracting unit which, by applying a voltage, attracts mist of the ink drop discharged from the print head, the mist attracting unit provided near the disc recording medium mounted to the disc mounting unit.

摘要翻译： 光盘记录和/或再现装置包括盘安装单元，盘式记录介质可拆卸地安装到该盘安装单元上，盘安装单元使盘记录介质旋转; 打印头，其朝向由盘安装单元旋转驱动的盘记录介质的标签表面排出墨滴; 以及雾吸引单元，其通过施加电压吸引从打印头排出的墨滴的雾，设置在安装在盘安装单元上的盘记录介质附近的雾吸引单元。

公开(公告)号：US20080303860A1

公开(公告)日：2008-12-11

申请号：US12156914

申请日：2008-06-05

申请人： Tatsumi Ito ,  Takeshi Matsui ,  Yuichiro Ikemoto

发明人： Tatsumi Ito ,  Takeshi Matsui ,  Yuichiro Ikemoto

IPC分类号： B41J2/165

CPC分类号： B41J3/4071 ,  B41J29/377 ,  B41J29/38

摘要： Disclosed is a liquid ejecting head includes a head main body having nozzles facing a spray surface of a rotating member rotationally driven, and ejecting liquid from the nozzles, and a mist capturing unit capturing mist of the liquid ejected from the nozzles. In the liquid ejecting head, the mist capturing unit is formed such that a passage, through which air flows in a predetermined direction over the spray surface while the rotating member is driven to rotate, is formed narrower than a distance between the nozzles and the spray surface.

摘要翻译： 公开了一种液体喷射头，包括：头主体，其具有面向旋转部件旋转驱动的喷射面的喷嘴，并且喷嘴喷射液体;以及雾捕获单元，其捕获从喷嘴喷出的液体的雾。 在液体喷射头中，雾状捕获单元被形成为使得当旋转构件被驱动旋转时空气在喷射表面上沿预定方向流动的通道形成为比喷嘴和喷雾之间的距离窄 表面。