公开(公告)号：US20090233367A1

公开(公告)日：2009-09-17

申请号：US12227405

申请日：2007-05-16

申请人： Kazuto Ikemoto ,  Yusuke Okubo ,  Kanako Koike ,  Sayaka Aizawa ,  Ichiro Sakata ,  Takafumi Sakai

发明人： Kazuto Ikemoto ,  Yusuke Okubo ,  Kanako Koike ,  Sayaka Aizawa ,  Ichiro Sakata ,  Takafumi Sakai

IPC分类号： C12N15/87 ,  C12N5/06 ,  C12M1/00

CPC分类号： C12N15/87

摘要： There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.

摘要翻译： 提供了通过方便的方法将期望转移到细胞中的多种类型的物质可以连续转移到多种类型的细胞中的方法，其中期望转移到细胞中的物质已被该方法吸收的细胞 ，以及通过该方法将物质转移到细胞中的装置。 上述目的可以通过用不含待转移到细胞中的物质的电池进行电喷雾来实现，同时细胞与要转移到细胞中的物质保持接触，或者用不含物质的液体的第一电喷雾细胞 转移到细胞中，然后使细胞与待转移进细胞的物质接触。

公开(公告)号：US07927874B2

公开(公告)日：2011-04-19

申请号：US12227405

申请日：2007-05-16

申请人： Kazuto Ikemoto ,  Yusuke Okubo ,  Kanako Koike ,  Sayaka Aizawa ,  Ichiro Sakata ,  Takafumi Skai

发明人： Kazuto Ikemoto ,  Yusuke Okubo ,  Kanako Koike ,  Sayaka Aizawa ,  Ichiro Sakata ,  Takafumi Skai

IPC分类号： C12N15/87

CPC分类号： C12N15/87

摘要： There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.

摘要翻译： 提供了通过方便的方法将期望转移到细胞中的多种类型的物质可以连续转移到多种类型的细胞中的方法，其中期望转移到细胞中的物质已被该方法吸收的细胞 ，以及通过该方法将物质转移到细胞中的装置。 上述目的可以通过用不含待转移到细胞中的物质的电池进行电喷雾来实现，同时细胞与要转移到细胞中的物质保持接触，或者用不含物质的液体的第一电喷雾池 转移到细胞中，然后使细胞与待转移进细胞的物质接触。

公开(公告)号：US08968237B2

公开(公告)日：2015-03-03

申请号：US13145269

申请日：2010-01-08

申请人： Kazuto Ikemoto ,  Takafumi Sakai ,  Kanako Koike ,  Satoshi Sakahara

发明人： Kazuto Ikemoto ,  Takafumi Sakai ,  Kanako Koike ,  Satoshi Sakahara

IPC分类号： A61M5/30 ,  H01R13/645 ,  H01R9/03 ,  H01R13/625 ,  H01R13/74

CPC分类号： H01R13/6453 ,  H01R9/03 ,  H01R13/625 ,  H01R13/748

摘要： The present invention provides a method whereby a drug can be conveniently injected into the living body, without sticking a needle, from a device that is not in contact with the living body, and a device therefor. Specifically provided is a method for injecting a peptide drug such as insulin, and a device therefor. No special consumables are needed except the drug solution or suspension. The method and device comprise injecting a drug into the living body by electrospraying a solution or suspension of the drug from a nozzle to which high voltage is applied and which is not in contact with the living body.

摘要翻译： 本发明提供一种方法，其可以将药物方便地从不与生物体接触的装置注入到生物体中，而不会使针头与其接触的装置及其装置。 具体提供了一种注射胰岛素等肽药物的方法及其装置。 除药液或悬浮液外，不需要特殊的消耗品。 该方法和装置包括通过电喷雾从施加高压的喷嘴并且不与生物接触的药液的溶液或悬浮液将药物注入生物体。

公开(公告)号：US20110275982A1

公开(公告)日：2011-11-10

申请号：US13145269

申请日：2010-01-08

申请人： Kazuto Ikemoto ,  Takafumi Sakai ,  Kanako Koike ,  Satoshi Sakahara

发明人： Kazuto Ikemoto ,  Takafumi Sakai ,  Kanako Koike ,  Satoshi Sakahara

IPC分类号： A61M37/00 ,  A61K38/02

CPC分类号： H01R13/6453 ,  H01R9/03 ,  H01R13/625 ,  H01R13/748

摘要： The present invention provides a method whereby a drug can be conveniently injected into the living body, without sticking a needle, from a device that is not in contact with the living body, and a device therefor. Specifically provided is a method for injecting a peptide drug such as insulin, and a device therefor. No special consumables are needed except the drug solution or suspension. The method and device comprise injecting a drug into the living body by electrospraying a solution or suspension of the drug from a nozzle to which high voltage is applied and which is not in contact with the living body.

摘要翻译： 本发明提供一种方法，其可以将药物方便地从不与生物体接触的装置注入到生物体中，而不会使针头与其接触的装置及其装置。 具体提供了一种注射胰岛素等肽药物的方法及其装置。 除药液或悬浮液外，不需要特殊的消耗品。 该方法和装置包括通过电喷雾从施加高压的喷嘴并且不与生物接触的药液的溶液或悬浮液将药物注入生物体。

公开(公告)号：US09163014B2

公开(公告)日：2015-10-20

申请号：US13383472

申请日：2010-06-09

申请人： Junichi Edahiro ,  Hitoshi Sakamoto ,  Kazuto Ikemoto ,  Hajime Shimizu ,  Tatsuya Hasegawa

发明人： Junichi Edahiro ,  Hitoshi Sakamoto ,  Kazuto Ikemoto ,  Hajime Shimizu ,  Tatsuya Hasegawa

IPC分类号： C07D471/04 ,  A23L1/30

CPC分类号： C07D471/04 ,  A23L33/10 ,  A23L33/15 ,  A23V2002/00 ,  C07B2200/13

摘要： A crystal of pyrroloquinoline quinone disodium salt having peaks at 2θ of 9.1°, 10.3°, 13.8°, 17.7°, 18.3°, 24.0°, 27.4°, 31.2° and 39.5° (±0.2° for each) in powder X-ray diffractometry using Cu Kα radiation, or a crystal of pyrroloquinoline quinone trisodium salt having peaks at 2θ of 6.6°, 11.4°, 13.0°, 22.6°, 26.9°, 27.9°, 37.0°, 38.9° and 43.4° (±0.2° for each) in powder X-ray diffractometry using Cu Kα radiation.

摘要翻译： 吡咯并喹啉醌二钠的晶体，其峰值在2℃。 在使用CuKα辐射的粉末X射线衍射中，为9.1°，10.3°，13.8°，17.7°，18.3°，24.0°，27.4°，31.2°和39.5°（±0.2°），或吡咯并喹啉 喹啉三钠盐在2℃具有峰值; 在使用CuKα辐射的粉末X射线衍射中，为6.6°，11.4°，13.0°，22.6°，26.9°，27.9°，37.0°，38.9°和43.4°（±0.2°）。

公开(公告)号：US20130253001A1

公开(公告)日：2013-09-26

申请号：US13989974

申请日：2011-11-25

申请人： Kazuto Ikemoto

发明人： Kazuto Ikemoto

IPC分类号： C07D471/04

CPC分类号： C07D471/04 ,  A61K31/475

摘要： An object of the present invention is to provide a salt of pyrroloquinoline quinone having a high solubility in water and in an organic solvent and a method for producing the same.According to the present invention, there are provided an ammonium salt of pyrroloquinoline quinone having a high solubility in water and in an organic solvent, consisting of a pyrroloquinoline quinone ion and an ammonium salt having a hydroxyl group.

摘要翻译： 本发明的目的是提供一种在水和有机溶剂中具有高溶解度的吡咯并喹啉醌及其制备方法。 根据本发明，提供了由吡咯并喹啉醌离子和具有羟基的铵盐组成的在水中和在有机溶剂中具有高溶解度的吡咯并喹啉醌的铵盐。

公开(公告)号：US20130203869A1

公开(公告)日：2013-08-08

申请号：US13814820

申请日：2011-08-09

申请人： Tsuyoshi Mikekado ,  Kazuto Ikemoto ,  Hajime Shimizu

发明人： Tsuyoshi Mikekado ,  Kazuto Ikemoto ,  Hajime Shimizu

IPC分类号： A23L1/054 ,  A61K9/06 ,  A61K8/04

CPC分类号： A23L1/054 ,  A23L29/20 ,  A23L33/10 ,  A61K8/0216 ,  A61K8/042 ,  A61K8/494 ,  A61K9/06 ,  A61K31/4745 ,  A61K2800/48 ,  A61K2800/84 ,  A61Q19/00 ,  Y10S514/944

摘要： An object of the present invention is to provide a gel containing pyrroloquinoline quinone that is capable of easily forming a gel near room temperature and is useful even in the food sector, and a method for producing the gel. According to the present invention, there is provided a gel containing pyrroloquinoline quinone that uses pyrroloquinoline quinone itself as a gelling agent and a method for producing the gel by adding a salt of pyrroloquinoline quinone to water and subsequently adjusting the temperature or the pH to reduce solubility.

摘要翻译： 本发明的目的是提供一种含有吡咯并喹啉醌的凝胶，其能够在室温附近容易地形成凝胶，并且甚至在食品部门中也是有用的，以及凝胶的制造方法。 根据本发明，提供一种使用吡咯并喹啉醌本身作为胶凝剂的吡咯并喹啉醌的凝胶，以及通过向吡啶并喹啉醌添加吡啶并喹啉醌的盐并随后调节温度或pH以降低溶解度来制备凝胶的方法 。

公开(公告)号：US08399256B2

公开(公告)日：2013-03-19

申请号：US13124434

申请日：2009-10-13

申请人： Kazuto Ikemoto

发明人： Kazuto Ikemoto

IPC分类号： C12N15/00 ,  C12N5/00 ,  C07D295/00

CPC分类号： C08G75/22 ,  C08L81/06

摘要： The present invention provides a method for transferring a gene into cells, which is practically useful and can achieve high transfer efficiency, and also a composition for use in the method. By bringing a mixture of a composition comprising a diallylamine sulfur dioxide copolymer having a repeating unit represented by the formula (1) or a salt thereof and a gene into contact with a cell, a gene such as DNA and RNA can be transferred into a target cell safely and conveniently, and into a cell at a specific site with high gene transfer efficiency.

摘要翻译： 本发明提供了将基因转移到细胞中的方法，其实际上是有用的并且可以实现高转移效率，以及用于该方法的组合物。 通过将包含具有式（1）表示的重复单元的二烯丙基胺二氧化硫共聚物或其盐和基因的组合物的混合物与细胞接触，可以将基因如DNA和RNA转移到靶 细胞安全方便地进入具有高基因转移效率的特定位点的细胞中。

公开(公告)号：US20110020935A1

公开(公告)日：2011-01-27

申请号：US12934139

申请日：2009-03-24

申请人： Kazuto Ikemoto

发明人： Kazuto Ikemoto

IPC分类号： C12N15/87 ,  C12M1/42

CPC分类号： C12M35/02 ,  C12M35/04 ,  C12N15/87

摘要： The present invention provides a method whereby, in transferring a gene into a cell by contacting the cell with a gene to be transferred into the cell in a container and then electrospraying a spray liquid free from the gene on the cell and gene in the container, the gene can be rapidly and conveniently transferred into the cell with high transfer efficiency while minimizing the degradation of the gene, and an apparatus therefor. A nozzle for electrospraying comprising a tube portion for applying a high voltage, which is made of an electrically conductive substance and located on the side of the spray liquid suction port, and another tube portion for spraying, which is made of an insulating substance and located on the side of the spray liquid ejection port. By using this nozzle, electrospraying can be performed while preventing a discharge phenomenon that causes degradation of the gene. Thus, transfer efficiency of the gene into the cell can be remarkably improved, and the gene can be rapidly and conveniently transferred into the cell.

摘要翻译： 本发明提供了一种方法，其通过使细胞与待转移到容器中的细胞中的基因接触而将基因转移到细胞中，然后电喷雾不含细胞上的基因和容器中的基因的喷雾液， 该基因可以快速方便地转移到具有高转移效率的细胞中，同时最小化基因的降解及其装置。 一种用于电喷雾的喷嘴，包括用于施加高电压的管部分，该管部分由导电物质制成并位于喷雾液体吸入口侧，另一个用于喷涂的管部分由绝缘物质制成并位于 在喷射液体喷出口侧。 通过使用该喷嘴，可以进行电喷雾，同时防止引起基因降解的放电现象。 因此，可以显着提高基因向细胞的转移效率，并且可以将基因快速方便地转移入细胞。

公开(公告)号：US07186844B2

公开(公告)日：2007-03-06

申请号：US11025934

申请日：2005-01-03

申请人： Kazuto Ikemoto

发明人： Kazuto Ikemoto

IPC分类号： C07D263/00 ,  C07D221/22

CPC分类号： C07D263/22 ,  C07D265/10

摘要： A method for producing a cyclic carbamate ester produces the cyclic carbamate ester by reacting an organic compound having at least two halogen atoms per molecule, an amine having at least two hydrogen atoms on a nitrogen atom, and carbon dioxide.

摘要翻译： 环状氨基甲酸酯的制造方法通过使每分子具有至少两个卤素原子的有机化合物，氮原子上具有至少2个氢原子的胺和二氧化碳反应，生成环状氨基甲酸酯。