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公开(公告)号：US5066462A
公开(公告)日：1991-11-19
申请号：US401494
申请日：1989-08-29
CPC分类号： C12Q1/48 , G01N33/525 , Y10S435/805
摘要： A dry-type analytical element suitable for measuring the activity of alanine aminotransferase in a liquid sample, characterized by incorporating a dye capable of absorbing the electromagnetic waves of 400 to 500 nm into at least one water-permeable layer. The coloring sensitivity of the dry-type analytical element does not increase substantially even under a fluorescent light, and thereby, an accurate measured value can easily be obtained.
摘要翻译： 适用于测量液体样品中丙氨酸氨基转移酶活性的干型分析元件，其特征在于将能够吸收400-500nm电磁波的染料掺入到至少一个透水层中。 即使在荧光下，干式分析元件的着色灵敏度也不会显着增加，从而可以容易地获得准确的测量值。
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公开(公告)号：US5508173A
公开(公告)日：1996-04-16
申请号：US373885
申请日：1995-01-17
CPC分类号： G01N33/525 , G01N33/526
摘要： A multilayer analytical element for measuring enzyme activity in a liquid sample such as a body fluid which is less affected by intrinsic pyruvic acid contained in the liquid sample is disclosed. The analytical element comprises a support, a reagent layer, an optionally provided other layer, and a porous spreading layer. The reagent layer and/or the optionally provided other layer contain pyruvic acid oxidase. The characteristic feature of the analytical element lies in that the spreading layer has a specific void volume in the range of 3-15 .mu.l/cm.sup.2.
摘要翻译： 公开了一种用于测量液体样品中的酶活性的多层分析元件，所述液体样品例如体液中较少受到液体样品中所含的固有丙酮酸的影响。 分析元件包括载体，试剂层，任选提供的其它层和多孔扩散层。 试剂层和/或任选提供的其它层含有丙酮酸氧化酶。 分析元件的特征在于扩散层具有在3-15μml/ cm 2范围内的特定空隙体积。
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公开(公告)号：US4503145A
公开(公告)日：1985-03-05
申请号：US352970
申请日：1982-02-26
申请人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
发明人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
IPC分类号： G01N33/66 , C12Q1/00 , C12Q1/26 , C12Q1/28 , C12Q1/48 , C12Q1/52 , G01N31/22 , G01N33/92 , G01N33/52
CPC分类号： C12Q1/52 , C12Q1/26 , Y10S435/805
摘要： An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.
摘要翻译： 用于测定转氨酶活性的分析膜包括含有丙酮酸氧化酶，氢供体和能够形成阳离子染料的成色剂的组合的试剂层和阴离子聚合物。 通过与用于检测过氧化氢的颜色指示剂组合物的反应形成的阳离子染料用阴离子聚合物固定，从而阻止阳离子染料的移动和扩散，使得分析膜实现高检测灵敏度和高精度。
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4.发明授权Quantitative analysis film and a method for colorimetric analysis using the same 失效定量分析膜和使用其的比色分析方法
公开(公告)号：US4478942A
公开(公告)日：1984-10-23
申请号：US352969
申请日：1982-02-26
申请人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
发明人： Harumi Katsuyama , Yoshikazu Amano , Asaji Kondo
CPC分类号： G01N33/523 , C12Q1/28 , C12Q2326/00 , C12Q2326/96 , Y10S435/805 , Y10S436/904 , Y10T436/206664
摘要： A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.
摘要翻译： 一种定量分析膜，其包含试剂层，其包含用于检测过氧化氢的阳离子染料形成颜色指示剂和阴离子聚合物。 通过使用这种颜色指示剂和阴离子聚合物组合，形成阳离子染料; 然后将形成的阳离子染料结合并固定到阴离子聚合物以提供增强的颜色。 还公开了高灵敏度和高效率的过氧化氢或其前体的比色测定方法。
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5.发明授权Dry analytical element having a spread control area for assaying enzyme activity 失效具有用于测定酶活性的扩散控制区的干燥分析元件
公开(公告)号：US4889797A
公开(公告)日：1989-12-26
申请号：US54432
申请日：1987-05-26
CPC分类号： G01N33/52 , C12Q1/32 , C12Q1/40 , C12Q1/42 , C12Q1/48 , Y10S435/805 , Y10S435/81 , Y10S436/808 , Y10S436/81
摘要： A dry analytical element for assaying an enzyme in a liquid is disclosed, comprising a support having provided thereon at least a porous liquid-spreading layer composed of water non-absorbent fibers, wherein said porous liquid-spreading layer contains a substrate for an enzyme to be assayed and a hydrophilic polymer in an amount effective to decrease a spreading area by at least 20%. The analytical element exhibits high sensitivity and high reproducibility.
摘要翻译： 公开了一种用于测定液体中的酶的干燥分析元件，其包括至少在由非水吸收性纤维构成的多孔液体扩散层上提供的支撑体，其中所述多孔液体扩散层含有用于酶的底物 并且将亲水性聚合物的量有效地将扩散面积减少至少20％。 分析元件显示出高灵敏度和高再现性。
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6.发明授权Analytical element having a polyacrylamide or derivative to minimize background for assaying enzyme activity 失效具有聚丙烯酰胺或衍生物的分析元件以最小化测定酶活性的背景
公开(公告)号：US5006458A
公开(公告)日：1991-04-09
申请号：US135670
申请日：1987-12-21
CPC分类号： G01N33/525 , Y10S435/805 , Y10S436/81 , Y10S436/904
摘要： A dry-type analytical element suitable for measuring enzyme activity of in a liquid sample, characterized by incorporating a polyacrylamide, polymethacrylamide or their derivatives into at least one water-permeable layer. The background concentration of the dry-type analytical element exhibits minimal increase even under a fluorescent light, and allows an accurate measured value to be easily obtained.
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公开(公告)号：US4604347A
公开(公告)日：1986-08-05
申请号：US577090
申请日：1984-02-06
CPC分类号： G01N33/525 , G01N31/221 , Y10S435/805
摘要： An analytical element containing at least one reagent for dry analysis of analyte in a liquid sample, which contains a nondiffusive acid and a nondiffusive base in different phases, being capable of adjusting the surrounding pH of the reaction area of at least one reagent under fluid contact therebetween, and said at least one reagent is contained in said nondiffusive acid-containing phase or nondiffusive base-containing phase.
摘要翻译： 包含至少一种用于干燥分析液体样品中的分析物的分析元件，其包含不同相中的非扩散性酸和非扩散性碱，能够调节流体接触下至少一种试剂的反应区域的周围pH值 并且所述至少一种试剂包含在所述非扩散性含酸相或非扩散性含碱相中。
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8.依法登记的发明
公开(公告)号：USH600H
公开(公告)日：1989-03-07
申请号：US171326
申请日：1988-03-21
IPC分类号： G01N33/52
CPC分类号： G01N33/525
摘要： A multilayer analytical element for determining an assay of a component in whole blood sample is disclosed, which comprises at least two water-permeable layers, wherein one layer is a sample spreading layer and the other layer is a reagent layer, wherein one layer or more of said water-permeable layers contain a composition which interacts with said component in whole blood sample, wherein the composition forms a dye capable of being detected by a spectrophotometry at a wavelength of 600 n.m. or longer, when the composition interacts with said component in whole blood sample.
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公开(公告)号：USH622H
公开(公告)日：1989-04-04
申请号：US195238
申请日：1988-05-18
IPC分类号： C12Q1/50
CPC分类号： C12Q1/50
摘要： In a dry analytical element for the determination of creatine kinase enzymatic activity is disclosed, comprising at least one porous medium and at least one water permeable layer having a liquid contact relation with the porous medium, the porous medium or the water permeable layer contains creatine phosphoric acid, ADP, a thiol compound as a creatine kinase activation agent, glucose, hexokinase, G6PDH, NAD.sup.+, an electron transmission agent and a formazane dye forming tetrazolium salt, the improvement wherein at least one water permeable layer having a liquid contact relation with the porous medium contains a formazane dye forming tetrazolium salt and not more than 16 g/m.sup.2 of gelatin.
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10.发明授权
公开(公告)号：US4900665A
公开(公告)日：1990-02-13
申请号：US185294
申请日：1988-04-19
CPC分类号： G01N33/526 , C12Q1/42 , C12Q2334/10 , Y10S435/805
摘要： An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing one or more compounds functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.
摘要翻译： 一种用于测量碱性磷酸酶活性的整体多层分析元件，其包含含有碱性磷酸酶敏感的自显影底物的机织织物或编织物的多孔扩散层，含有一种或多种用作磷酸盐的化合物的缓冲层 酸受体和缓冲剂和层叠形式的支撑层。
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