公开(公告)号：US07321032B2

公开(公告)日：2008-01-22

申请号：US10543491

申请日：2004-08-12

申请人： Kazuyoshi Namba ,  Michiko Hatano ,  Tomoko Yaeshima ,  Norio Ishibashi ,  Yoshitaka Tamura

发明人： Kazuyoshi Namba ,  Michiko Hatano ,  Tomoko Yaeshima ,  Norio Ishibashi ,  Yoshitaka Tamura

IPC分类号： C07H21/04 ,  C12P19/34 ,  G01N31/22

CPC分类号： C12Q1/689

摘要： PCR primers for detecting Bifidobacterium infantis comprising: a pair of a first oligonucleotide having a sequence of 20 or more continuous nucleotides selected from a nucleotide sequence of SEQ ID NO: 1 and a second oligonucleotide having a nucleotide sequence of SEQ ID NO: 2 or a nucleotide sequence of SEQ ID NO: 2 in which 0 to 7 nucleotides has been deleted from its 5′-terminal side is used to perform PCR using a chromosomal DNA or 16S rRNA of test bacteria as a template, and whether an amplicon of Bifidobacterium infantis is present or not is determined.

摘要翻译： 用于检测婴儿双歧杆菌的PCR引物包含：一对具有选自SEQ ID NO：1的核苷酸序列的20个以上连续核苷酸序列的第一寡核苷酸和具有核苷酸序列SEQ ID NO：2的第二寡核苷酸或 使用其5'末端侧缺失了0〜7个核苷酸的序列号2的核苷酸序列进行PCR，使用测试细菌的染色体DNA或16S rRNA作为模板，以及婴儿双歧杆菌的扩增子 是否存在确定。

公开(公告)号：US20060281084A1

公开(公告)日：2006-12-14

申请号：US10543491

申请日：2004-08-12

申请人： Kazuyoshi Namba ,  Michiko Hatano ,  Tomoko Yaeshima

发明人： Kazuyoshi Namba ,  Michiko Hatano ,  Tomoko Yaeshima

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689

摘要： PCR primers for detecting Bifidobacterium infantis comprising: a pair of a first oligonucleotide having a sequence of 20 or more continuous nucleotides selected from a nucleotide sequence of SEQ ID NO: 1 and a second oligonucleotide having a nucleotide sequence of SEQ ID NO: 2 or a nucleotide sequence of SEQ ID NO: 2 in which 0 to 7 nucleotides has been deleted from its 5′-terminal side is used to perform PCR using a chromosomal DNA or 16S rRNA of test bacteria as a template, and whether an amplicon of Bifidobacterium infantis is present or not is determined.

摘要翻译： 用于检测婴儿双歧杆菌的PCR引物包含：一对具有选自SEQ ID NO：1的核苷酸序列的20个以上连续核苷酸序列的第一寡核苷酸和具有核苷酸序列SEQ ID NO：2的第二寡核苷酸或 使用其5'末端侧缺失了0〜7个核苷酸的序列号2的核苷酸序列进行PCR，使用测试细菌的染色体DNA或16S rRNA作为模板，以及婴儿双歧杆菌的扩增子 是否存在确定。

公开(公告)号：US4588595A

公开(公告)日：1986-05-13

申请号：US547827

申请日：1983-11-02

申请人： Shigeo Okonogi ,  Takuji Kawashima ,  Tsutomu Kudo ,  Hiroya Yuguchi ,  Akinori Hiramatsu ,  Susumu Teraguchi ,  Tomoko Yaeshima

发明人： Shigeo Okonogi ,  Takuji Kawashima ,  Tsutomu Kudo ,  Hiroya Yuguchi ,  Akinori Hiramatsu ,  Susumu Teraguchi ,  Tomoko Yaeshima

IPC分类号： A23C9/12 ,  A23C9/123 ,  C12N1/20 ,  C12R1/01 ,  C12R1/225 ,  C12R1/46 ,  A23C9/127 ,  A23C23/00

CPC分类号： A23C9/1234 ,  A23C9/1238 ,  C12N1/20 ,  Y10S435/885

摘要： A culture containing a viable mass of Bifodobacteria and lactic acid bacteria is obtained by incubating separately or together microorganisms of the genus Bifidobacterium and Lactobacillus, and the species Streptococcus thermophilus in a medium containing milk as the main ingredient. The Streptococcus thermophilus is a strain having high oxygen uptake. When incubated separately, the microorganisms are mixed together after incubating. In the presence of the high oxygen uptake Streptococcus thermophilus, a high survival fraction of the Bifidobacteria is obtained when the culture is stored under aerobic conditions. The culture is useful in preparing food, medicine or feed.

摘要翻译： 通过将含有双歧杆菌属和乳杆菌属的微生物和嗜热链球菌属物种在含有乳作为主要成分的培养基中分别或一起孵育来获得含有双歧杆菌和乳酸菌的活体的培养物。 嗜热链球菌是具有高氧摄取的菌株。 孵育后，将微生物混合在一起。 在高吸氧链嗜热链球菌的存在下，当培养物在有氧条件下储存时，获得双歧杆菌的高存活率。 该文化对于准备食物，药物或饲料是有用的。

公开(公告)号：US08338161B2

公开(公告)日：2012-12-25

申请号：US12162705

申请日：2007-11-02

申请人： Kanetada Shimizu ,  Tomoko Yaeshima

发明人： Kanetada Shimizu ,  Tomoko Yaeshima

IPC分类号： C12N1/20

CPC分类号： C12N1/38 ,  A23C9/1234 ,  A23L33/135 ,  A23V2002/00 ,  A23Y2220/49 ,  A23Y2300/55 ,  A61K35/744 ,  C12N1/20 ,  C12R1/46 ,  A23V2200/3204 ,  A23V2200/32

摘要： The present invention relates to bacteria of a genus Lactococcus having bacteriological properties of: (1) fermentability which curdles a 10% (W/W) reconstituted skim milk medium when cultivated at a temperature of 25° C. to 37° C. for 16 hours; (2) Bifidobacterium longum growth-promoting properties which lead to a viable count of Bifidobacterium longum of 5×108 CFU/g or more, when co-cultivated with Bifidobacterium longum in the 10% (W/W) reconstituted skim milk medium, until the pH thereof is 4.4 to 4.6; and (3) Bifidobacterium longum survivability-improving properties during storage, which lead to a survival rate of Bifidobacterium longum of 30% or more, after co-cultivation with Bifidobacterium longum in the 10% (W/W) reconstituted skim milk medium until the pH thereof is 4.4 to 4.6, rapid cooling, and two weeks storage at 10° C.

摘要翻译： 本发明涉及一种具有细菌学特性的细菌的细菌性质：（1）发酵能力，其在25℃至37℃的温度下培养16天时，将10％（W / W）重构脱脂乳培养基凝结为16 小时; （2）当在10％（W / W）重构的脱脂乳培养基中与长双歧杆菌共培养时，长双歧杆菌生长促进特性导致5×108CFU / g以上的长双歧杆菌的活菌计数直到 其pH为4.4〜4.6; 和（3）在长期保存双歧杆菌存活率方面，在10％（W / W）重构的脱脂乳培养基中与长双歧杆菌共同培养后，长寿双歧杆菌的存活率达30％以上，直至 pH值为4.4至4.6，快速冷却，并在10℃下储存两周。

公开(公告)号：US20100086988A1

公开(公告)日：2010-04-08

申请号：US12162705

申请日：2007-11-02

申请人： Kanetada Shimizu ,  Tomoko Yaeshima

发明人： Kanetada Shimizu ,  Tomoko Yaeshima

IPC分类号： C12N1/20

CPC分类号： C12N1/38 ,  A23C9/1234 ,  A23L33/135 ,  A23V2002/00 ,  A23Y2220/49 ,  A23Y2300/55 ,  A61K35/744 ,  C12N1/20 ,  C12R1/46 ,  A23V2200/3204 ,  A23V2200/32

摘要： The present invention relates to bacteria of a genus Lactococcus having bacteriological properties of: (1) fermentability which curdles a 10% (W/W) reconstituted skim milk medium when cultivated at a temperature of 25° C. to 37° C. for 16 hours; (2) Bifidobacterium longum growth-promoting properties which lead to a viable count of Bifidobacterium longum of 5×108 CFU/g or more, when co-cultivated with Bifidobacterium longum in the 10% (W/W) reconstituted skim milk medium, until the pH thereof is 4.4 to 4.6; and (3) Bifidobacterium longum survivability-improving properties during storage, which lead to a survival rate of Bifidobacterium longum of 30% or more, after co-cultivation with Bifidobacterium longum in the 10% (W/W) reconstituted skim milk medium until the pH thereof is 4.4 to 4.6, rapid cooling, and two weeks storage at 10° C.

摘要翻译： 本发明涉及一种具有细菌学特性的细菌的细菌性质：（1）发酵能力，其在25℃至37℃的温度下培养16天时，将10％（W / W）重构脱脂乳培养基凝结为16 小时; （2）当在10％（W / W）重构的脱脂乳培养基中与长双歧杆菌共培养时，长双歧杆菌生长促进特性导致5×108CFU / g以上的长双歧杆菌的活菌计数直到 其pH为4.4〜4.6; 和（3）在长期保存双歧杆菌存活率方面，在10％（W / W）重构的脱脂乳培养基中与长双歧杆菌共同培养后，长寿双歧杆菌的存活率达30％以上，直至 pH值为4.4至4.6，快速冷却，并在10℃下储存两周。

公开(公告)号：US20120149054A1

公开(公告)日：2012-06-14

申请号：US13390859

申请日：2011-03-25

申请人： Masimichi Muto ,  Fumiaki Abe ,  Tomoko Yaeshima

发明人： Masimichi Muto ,  Fumiaki Abe ,  Tomoko Yaeshima

IPC分类号： C12Q1/02

CPC分类号： C12Q1/045 ,  C12N1/20 ,  C12Q1/06 ,  G01N2333/36

摘要： There are provided a method of identifying the longum species alone, such as Bifidobacterium longum subsp. longum alone, and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains L-arabinose as a sole sugar source; and also a culture medium which is useful as a selective medium for the above measurement method and which is also easy to prepare.

摘要翻译： 提供了一种识别长单独物种的方法，例如长双歧杆菌（Bifidobacterium longum subsp。） 并且通过使用含有L-阿拉伯糖作为唯一糖源的培养基，测量其中包含属于双歧杆菌属的微生物的待测试细菌细胞中的活细胞数; 以及作为上述测定方法的选择培养基有用的培养基，也容易制备。

公开(公告)号：US4601985A

公开(公告)日：1986-07-22

申请号：US547825

申请日：1983-11-02

申请人： Shigeo Okonogi ,  Jyoji Ono ,  Tsutomu Kudo ,  Akinori Hiramatsu ,  Susumu Teraguchi ,  Tomoko Yaeshima

发明人： Shigeo Okonogi ,  Jyoji Ono ,  Tsutomu Kudo ,  Akinori Hiramatsu ,  Susumu Teraguchi ,  Tomoko Yaeshima

IPC分类号： A23C9/123 ,  A23C9/127 ,  A61K35/74 ,  A61P1/12 ,  C12N1/04 ,  C12N1/20 ,  C12P39/00

CPC分类号： C12R1/46 ,  A23C9/1234 ,  A23C9/1238 ,  C12N1/04 ,  C12N1/20 ,  C12P39/00 ,  Y10S435/822 ,  Y10S435/885

摘要： This invention relates to a new microorganism belonging to Streptococcus thermophilus having an oxygen uptake ability of at least 30 nano moles per milligram of dried cell of said microorganism per minute which is defined by the quantity of oxygen consumption as determined by Warburg's manometric method, and to a microbial composition which comprises as main ingredients viable cell mass of (a) said microorganism and (b) bifidobacteria.

摘要翻译： 本发明涉及属于嗜热链球菌的新微生物，其具有每分钟至少30纳摩尔每毫克所述微生物干燥细胞的氧吸收能力，其由通过Warburg's测量方法确定的耗氧量定义，以及 一种微生物组合物，其包含（a）所述微生物和（b）双歧杆菌的主要成分活细胞块。

公开(公告)号：US08859226B2

公开(公告)日：2014-10-14

申请号：US13390859

申请日：2011-03-25

申请人： Masamichi Muto ,  Fumiaki Abe ,  Tomoko Yaeshima

发明人： Masamichi Muto ,  Fumiaki Abe ,  Tomoko Yaeshima

IPC分类号： C12N1/20 ,  C12Q1/06 ,  C12Q1/04

CPC分类号： C12Q1/045 ,  C12N1/20 ,  C12Q1/06 ,  G01N2333/36

摘要： There are provided a method of identifying the longum species alone, such as Bifidobacterium longum subsp. longum alone, and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains L-arabinose as a sole sugar source; and also a culture medium which is useful as a selective medium for the above measurement method and which is also easy to prepare.

摘要翻译： 提供了一种识别长单独物种的方法，例如长双歧杆菌（Bifidobacterium longum subsp。） 并且通过使用含有L-阿拉伯糖作为唯一糖源的培养基，测量其中包含属于双歧杆菌属的微生物的待测试细菌细胞中的活细胞数; 以及作为上述测定方法的选择培养基有用的培养基，也容易制备。

公开(公告)号：US20120244571A1

公开(公告)日：2012-09-27

申请号：US13391855

申请日：2011-03-25

申请人： Masamichi Muto ,  Fumiaki Abe ,  Tomoko Yaeshima

发明人： Masamichi Muto ,  Fumiaki Abe ,  Tomoko Yaeshima

IPC分类号： C12Q1/06

CPC分类号： C12Q1/06 ,  C12N1/20 ,  C12Q1/045 ,  G01N2333/195

摘要： There are provided a method of identifying a microorganism belonging to Bifidobacterium breve alone and measuring the viable cell count thereof, amongst the bacterial cells to be tested which include a microorganism belonging to the genus Bifidobacterium by using a culture medium that contains at least one sugar alcohol selected from sorbitol and mannitol as a sole sugar source; and also a culture medium which is useful as a selective medium to be used in the above measurement method and which is also easy to prepare.

摘要翻译： 提供一种通过使用含有至少一种糖醇的培养基来鉴定属于双歧杆菌的微生物在测定的细菌细胞中测定活细胞数的方法，所述细菌细胞包括双歧杆菌属的微生物 选自山梨糖醇和甘露醇作为唯一的糖源; 以及作为上述测定方法中使用的也易于制备的选择性培养基的培养基。

公开(公告)号：US07427499B2

公开(公告)日：2008-09-23

申请号：US10709674

申请日：2004-05-21

申请人： Kazuyoshi Sotoyama ,  Akinori Hiramatsu ,  Jin-zhong Xiao ,  Noritoshi Takahashi ,  Shizuki Kondo ,  Tomoko Yaeshima ,  Sachiko Takahashi

发明人： Kazuyoshi Sotoyama ,  Akinori Hiramatsu ,  Jin-zhong Xiao ,  Noritoshi Takahashi ,  Shizuki Kondo ,  Tomoko Yaeshima ,  Sachiko Takahashi

IPC分类号： C12N1/20

CPC分类号： A23C9/1234 ,  A23L29/065 ,  A23L33/135 ,  A23Y2220/13 ,  A23Y2300/55

摘要： 17It is intended to provide Bifidobacterium longum having the following bacteriological properties and microbial powders, foods and beverages containing this Bifidobacterium longum: (1) having such fermentation ability as to make a milk base medium to pH 4.6 or below; (2) showing a survival rate of 50% or more in the case of, after attaining pH 4.4 to 4.6 in a milk base medium, quenching and storing at 10° C. for two weeks; and (3) having such gastric juice tolerance as to yield a survival rate of 10% or more in the case of storing in artificial gastric juice (pH 3.0) at 37° C. for two hours.

摘要翻译： 本发明旨在提供具有以下细菌学特性的长双歧杆菌和含有这种长双歧杆菌的微生物粉末，食品和饮料：（1）具有如下发酵能力使得乳基培养基达到pH4.6或更低; （2）在乳基培养基中达到pH4.4〜4.6后，存活率为50％以上，在10℃下淬火储存2周; 和（3）具有这样的胃液耐受性，在将人造胃液（pH3.0）在37℃下储存2小时的情况下，生存率为10％以上。