Stabilized lyophilized mammalian cells and method of making same
    1.
    发明授权
    Stabilized lyophilized mammalian cells and method of making same 失效
    稳定的LYOPHILIZAM MAMMALIAN细胞及其制备方法

    公开(公告)号:US5059518A

    公开(公告)日:1991-10-22

    申请号:US260260

    申请日:1988-10-20

    摘要: A method of lyophilizing mammalian cells to produce preserved human cells, hybridoma cell lines, tissue cells, and control cells for immunoassays and other hematological measurements. Prior to freezing and lyophilizing, the prepared mammalian cell pellet is suspended in a solution of trehalose in an isotonic fluid prepared at a specified optimal concentration and incubated at room temperature for a designated time period. The lyophilized cells when rehydrated, retain their optimal physiological characteristics suitable for use as an analytical control and retain said characteristics after storage at 2.degree.-8.degree. C. for a period in excess of five (5) months.

    摘要翻译: 冻干哺乳动物细胞以产生保存的人细胞,杂交瘤细胞系,组织细胞和用于免疫测定和其他血液学测量的对照细胞的方法。 在冷冻和冻干之前,将所制备的哺乳动物细胞沉淀物悬浮于海藻糖溶液中,所述溶液以规定的最佳浓度制备的等渗液体中,并在室温下温育指定的时间段。 当再次水化时,冻干细胞保留其适合用作分析对照的最佳生理特征,并在2℃-8℃下储存超过五(5)个月后保留所述特征。