利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.019 秒)

    Method for Identifying Within a Mammal a DNA Encoding A Physiologically Active Polypeptide
    1.
    发明申请
    Method for Identifying Within a Mammal a DNA Encoding A Physiologically Active Polypeptide 审中-公开
    用于在哺乳动物中识别编码生理活性多肽的DNA的方法

    公开(公告)号：US20100329988A1

    公开(公告)日：2010-12-30

    申请号：US12858960

    申请日：2010-08-18

    申请人： Kiyoshi Habu ,  Kou-ichi Jishage ,  Hiroshi Suzuki

    发明人： Kiyoshi Habu ,  Kou-ichi Jishage ,  Hiroshi Suzuki

    IPC分类号： A61K49/00 ,  A61P43/00

    CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/1034 ,  G01N33/5088

    摘要： The present inventors found that a physiological effect of a particular DNA can be detected independently within mice into which a pool of various DNAs in various quantities has been introduced. This finding suggests that it is possible to identify a DNA having a particular physiological effect by successively fractionating a pool of various DNAs in various quantities using the particular physiological effect seen within a mammal as an index. Such a method of screening will have the advantage of saving much time and effort as required in conventional screenings such as those utilizing transgenic and knockout mice. Furthermore, the method of screening has the additional advantage of enabling the identification of a DNA having a physiological activity, for example, even when the cells producing a physiologically active substance cannot be maintained in vitro or in immunodeficient animals, or when the cells change their characteristics during passage and stop producing the physiologically active substance.

    摘要翻译： 本发明人发现，在引入了各种量的各种DNA的小鼠中，可以独立地检测特定DNA的生理作用。 该发现表明，可以通过使用哺乳动物中看到的特定生理效应作为指标，以各种量依次分级各种DNA的池来鉴定具有特定生理效应的DNA。 这种筛选方法将具有如常规筛选（例如使用转基因和敲除小鼠的筛选）所需的大量时间和精力的优点。 此外，筛选方法具有能够鉴定具有生理活性的DNA的额外优点，例如即使当生产生理活性物质的细胞不能在体外或免疫缺陷动物中维持时，或者当细胞改变其 特性在通过和停止生产生理活性物质。

    Hcv gene transgenic animal
    5.
    发明申请
    Hcv gene transgenic animal 审中-公开
    Hcv基因转基因动物

    公开(公告)号：US20060174354A1

    公开(公告)日：2006-08-03

    申请号：US10494177

    申请日：2002-09-24

    申请人： Michinori Kohara ,  Hiroshi Suzuki ,  Otoya Ueda ,  Kou-ichi Jishage ,  Asao Katsume

    发明人： Michinori Kohara ,  Hiroshi Suzuki ,  Otoya Ueda ,  Kou-ichi Jishage ,  Asao Katsume

    IPC分类号： A01K67/027

    CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0337 ,  C07K14/005 ,  C12N2770/24222 ,  C12N2800/30

    摘要： The present invention provides a transgenic animal expressing the full-length human hepatitis C virus (HCV) for the purpose of constructing a system for screening for a remedy for human hepatitis C. The present invention is a transgenic hepatitis C model animal, which has the full-length DNA of the hepatitis C virus incorporated therein and can express the full-length HCV gene, and a method of generating a hepatitis C model animal carrying the full-length HCV gene, which comprises introducing a vector containing the full-length DNA of hepatitis C virus into an ES cell, and causing the ES cells to undergo ontogenesis in a pseudo-parent.

    摘要翻译： 本发明提供了表达全长人丙型肝炎病毒（HCV）的转基因动物，目的是构建用于筛选人丙型肝炎的药物的系统。本发明是转基因丙型肝炎模型动物，其具有 其中并入丙型肝炎病毒的全长DNA并能表达全长HCV基因，以及产生携带全长HCV基因的丙型肝炎模型动物的方法，其包括引入含有全长DNA的载体 的丙型肝炎病毒进入ES细胞，并使得ES细胞在假亲本中经历发生。