公开(公告)号：US5225343A

公开(公告)日：1993-07-06

申请号：US603361

申请日：1990-10-26

申请人： Ko Sakata ,  Eitaroh Morita ,  Tetsuya Takezono

发明人： Ko Sakata ,  Eitaroh Morita ,  Tetsuya Takezono

IPC分类号： A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C12P17/18

CPC分类号： C12N5/04 ,  A01H4/00 ,  C12N5/0025 ,  C12P17/181

摘要： A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, culturing the differentiated organ on a solid medium to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound.

公开(公告)号：US5336605A

公开(公告)日：1994-08-09

申请号：US11671

申请日：1993-02-01

申请人： Ko Sakata ,  Eitaroh Morita ,  Tetsuya Takezono

发明人： Ko Sakata ,  Eitaroh Morita ,  Tetsuya Takezono

IPC分类号： A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C12P17/18 ,  C12N5/02

CPC分类号： C12N5/04 ,  A01H4/00 ,  C12N5/0025 ,  C12P17/181

摘要： A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, culturing the differentiated organ on a solid medium to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound.

摘要翻译： 一种制备属于Podophyllum属的植物的外来胚胎的方法，包括以下步骤：制备属于Podophyllum属植物的细胞，将无细胞培养基中的细胞或含有高达2％的培养基 重量糖，然后将细胞在含有超过2重量％糖的培养基中培养以产生外来胚胎; 一种生产鬼臼毒素化合物的方法，包括以下步骤：制备属于Podophyllum属植物的外来胚胎，培养外来胚胎以产生外来器官，培养外来器官以产生鬼臼毒素化合物，并回收鬼臼毒素 复合; 以及生产鬼臼毒素化合物的方法，包括以下步骤：在液体培养基中培养属于茯苓属的植物的去分化细胞或组织培养分化的细胞或组织以产生分化的器官，培养 分离的器官在固体培养基上产生鬼臼毒素化合物，并回收鬼臼毒素化合物。

公开(公告)号：US5318906A

公开(公告)日：1994-06-07

申请号：US893647

申请日：1992-06-04

申请人： Ko Sakata ,  Tetsuya Taekzono ,  Noritsugu Yabe ,  Hisao Matsui

发明人： Ko Sakata ,  Tetsuya Taekzono ,  Noritsugu Yabe ,  Hisao Matsui

IPC分类号： A61K35/14 ,  C12N5/02 ,  C12N5/0783 ,  C12N5/00 ,  A61K35/78 ,  C12P1/00 ,  C12Q1/00

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K36/258 ,  A61K2039/5158 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2501/23

摘要： An agent for stimulating growth of animal cells in vitro comprises ginseng or an active ingredient extracted from ginseng Serum-free culture media containing the agent are disclosed. The present serum-free media contain less impure protein than serum-containing media, so that isolation and purification of substances produced by animal cells cultured therein is facilitated. The present serum-free media enhance the growth of lymphocytes in vitro, and can be used for vital cell transplant therapy, particularly for adoptive immunotherapy.

摘要翻译： 用于刺激体内动物细胞生长的试剂包括人参或从人参提取的活性成分公开了含有该药剂的无血清培养基。 目前的无血清培养基含有比含血清培养基少的不纯的蛋白质，从而促进了在其中培养的动物细胞产生的物质的分离和纯化。 目前的无血清培养基体外增强淋巴细胞的生长，可用于重要的细胞移植治疗，特别是过继免疫治疗。

公开(公告)号：US4525457A

公开(公告)日：1985-06-25

申请号：US487574

申请日：1983-04-22

申请人： Ko Sakata ,  Hirosuke Imai

发明人： Ko Sakata ,  Hirosuke Imai

IPC分类号： C12N11/08 ,  C12N11/10

CPC分类号： C12N11/10 ,  C12N11/08

摘要： This invention discloses a method of immobilizing an enzymatically active material to a water-insoluble polyanion which comprises bringing a polycation-containing aqueous medium and the enzymatically active substance into contact with the water-insoluble polyanion having any desired form. This method is characterized in that the enzymatically active substance is adsorbed on the surfaces of the water-insoluble polyanion and, at the same time, a strong complex is formed by the interaction of the polycation and the polyanion to produce a water-insoluble film. As a result, the enzymatically active substance so adsorbed is securely fixed to the surfaces of the water-insoluble polyanion and scarcely released therefrom.

摘要翻译： 本发明公开了一种将酶活性物质固定在水不溶性聚阴离子中的方法，该方法包括使含聚阳离子的含水介质和酶活性物质与具有任何所需形式的水不溶性聚阴离子接触。 该方法的特征在于，酶活性物质被吸附在水不溶性聚阴离子的表面上，同时通过聚阳离子和聚阴离子的相互作用形成强络合物，从而产生水不溶性膜。 结果，如此吸附的酶活性物质牢固地固定在水不溶性聚阴离子的表面上，几乎不从其中释放出来。

公开(公告)号：US4520178A

公开(公告)日：1985-05-28

申请号：US398589

申请日：1982-07-15

申请人： Ko Sakata ,  Hirosuke Imai

发明人： Ko Sakata ,  Hirosuke Imai

IPC分类号： A61L15/60 ,  C08F2/18 ,  C08F2/20 ,  C12N11/04

CPC分类号： C12N11/04 ,  A61L15/60 ,  C08F2/18

摘要： A process for producing a water-insoluble polymer of uniform shape having high strength and a desired geometric form is disclosed. This process comprises the steps of dissolving or partially suspending in water (a) a water-soluble salt of alginic acid, (b) a hydrophilic, crosslinkable vinyl monomer having two or more radical-polymerizable vinyl groups per molecule, and (c) optionally a hydrophilic, radical-polymerizable vinyl monomer; bringing the resulting aqueous solution or suspension into contact with an aqueous solution capable of causing gelation of the water-soluble salt of alginic acid and thereby forming an water-insoluble gel of uniform shape; and subjecting the gel to radical polymerization to produce a water-insoluble polymer.

摘要翻译： 公开了一种具有高强度和所需几何形状的具有均匀形状的水不溶性聚合物的方法。 该方法包括以下步骤：将水溶解或部分悬浮在水中（a）藻酸的水溶性盐，（b）每分子具有两个或更多个可自由基聚合的乙烯基的亲水性，可交联的乙烯基单体，和（c）任选地 亲水的，可自由基聚合的乙烯基单体; 使得到的水溶液或悬浮液与能够引起藻酸的水溶性盐的凝胶化的水溶液接触，从而形成均匀形状的水不溶性凝胶; 并使凝胶进行自由基聚合以产生水不溶性聚合物。

公开(公告)号：US4208540A

公开(公告)日：1980-06-17

申请号：US43186

申请日：1979-05-29

申请人： Masao Imaizumi ,  Mitsuo Yasuda ,  Ko Sakata ,  Noboru Hirano

发明人： Masao Imaizumi ,  Mitsuo Yasuda ,  Ko Sakata ,  Noboru Hirano

IPC分类号： C07C7/148 ,  C07C29/04 ,  C07C7/01

CPC分类号： C07C29/04 ,  C07C7/148

摘要： A process for the continuous separation and recovery of isobutene from an isobutene-containing hydrocarbon mixture, comprising the steps of contacting the mixture with water to obtain a tertiary butanol-containing mixture, separating this mixture into hydrocarbon and aqueous layer, distilling the hydrocarbon layer to obtain a tertiary butanol-rich stream, dehydrating the thus obtained stream to obtain an isobutene-containing gaseous mixture and then introducing the thus obtained mixture into a distillation means to recover isobutene as the product therefrom.

摘要翻译： 一种用于从含异丁烯的烃混合物中连续分离和回收异丁烯的方法，包括以下步骤：使混合物与水接触以获得含叔丁醇的混合物，将该混合物分离成烃和水层，将烃层蒸馏至 得到富含叔丁醇的物流，将由此得到的物流脱水得到含异丁烯的气体混合物，然后将由此获得的混合物引入蒸馏装置中以从其中回收异丁烯。

公开(公告)号：US4636229A

公开(公告)日：1987-01-13

申请号：US712001

申请日：1985-03-14

申请人： Hiroyuki Itoh ,  Ko Sakata ,  Hirosuke Imai

发明人： Hiroyuki Itoh ,  Ko Sakata ,  Hirosuke Imai

IPC分类号： B01D71/40 ,  B01D53/22

CPC分类号： B01D71/40

摘要： A selective gas separation membrane comprising a fumaric diester polymer having a repeating unit represented by the following general formula (1): ##STR1## wherein X and Y are each independently an alkyl, cycloalkyl, aryl, trialkylsilylalkyl or siloxane-containing alkyl group.

摘要翻译： 一种选择性气体分离膜，其包含具有由以下通式（1）表示的重复单元的富马酸二酯聚合物：其中X和Y各自独立地为烷基，环烷基，芳基，三烷基甲硅烷基烷基或含硅氧烷的烷基 组。

公开(公告)号：US4180688A

公开(公告)日：1979-12-25

申请号：US928294

申请日：1978-07-26

申请人： Masao Imaizumi ,  Mitsuo Yasuda ,  Ko Sakata ,  Noboru Hirano

发明人： Masao Imaizumi ,  Mitsuo Yasuda ,  Ko Sakata ,  Noboru Hirano

IPC分类号： C07C29/04

CPC分类号： C07C29/04

摘要： A continuous method for producing tert-butyl alcohol from water and isobutylene in the presence of a strongly acidic cation exchange resin as a catalyst is characterized by: said catalyst particles being filled in a reactor, a liquid isobutylene being continuously brought into contact with water such that said liquid isobutylene is filled in a continuous phase in the gaps between said catalyst particles, and that water streams downwardly along the surface of said catalyst particle and subsequently the reaction mixture being continuously taken out from the reactor. According to the method of this invention, tert-butyl alcohol is obtained in a high yield with little formation of by-products.

摘要翻译： 在强酸性阳离子交换树脂作为催化剂存在下，从水和异丁烯生产叔丁醇的连续方法的特征在于：所述催化剂颗粒填充在反应器中，液体异丁烯与水不断接触， 所述液体异丁烯在所述催化剂颗粒之间的间隙中以连续相填充，并且水沿着所述催化剂颗粒的表面向下流动，随后将反应混合物从反应器中连续取出。 根据本发明的方法，以高产率获得叔丁醇，几乎不形成副产物。