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公开(公告)号：US08475633B2
公开(公告)日：2013-07-02
申请号：US13056922
申请日：2009-04-14
申请人： Kwang Hoe Chung , Sung Yu Hong , Hyun Ju Doh , Jin Sub Choi , Jae Hoon Lim , Sung Joong Kim
发明人： Kwang Hoe Chung , Sung Yu Hong , Hyun Ju Doh , Jin Sub Choi , Jae Hoon Lim , Sung Joong Kim
IPC分类号： C23C14/08
CPC分类号： G01N33/4905
摘要： Provided is a method for preparing an epoxy substrate having a nanopattern, including: (a) forming a titanium oxide film by anodizing a titanium substrate; (b) obtaining a titanium substrate having a concave shape formed on the surface by removing the titanium oxide film from the titanium substrate on which the titanium oxide film has been formed; (c) coating an epoxy resin onto the titanium substrate on which the concave shape has been formed; and (d) obtaining an epoxy substrate having a nanopattern of convex surfaces by removing the titanium substrate.
摘要翻译： 提供一种制备具有纳米图案的环氧树脂基板的方法，包括：（a）通过阳极氧化钛基板形成氧化钛膜; （b）从形成有氧化钛膜的钛基板除去氧化钛膜，得到表面形成有凹状的钛基板; （c）将环氧树脂涂布到已经形成凹形的钛基板上; 和（d）通过除去钛基材得到具有凸表面的纳米图案的环氧树脂基材。
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公开(公告)号：US20110155591A1
公开(公告)日：2011-06-30
申请号：US13056922
申请日：2009-04-14
申请人： Kwang Hoe Chung , Sung Yu Hong , Hyun Ju Doh , Jin Sub Choi , Jae Hoon Lim , Sung Joong Kim
发明人： Kwang Hoe Chung , Sung Yu Hong , Hyun Ju Doh , Jin Sub Choi , Jae Hoon Lim , Sung Joong Kim
CPC分类号： G01N33/4905
摘要： Provided is a method for preparing an epoxy substrate having a nanopattern, including: (a) forming a titanium oxide film by anodizing a titanium substrate; (b) obtaining a titanium substrate having a concave shape formed on the surface by removing the titanium oxide film from the titanium substrate on which the titanium oxide film has been formed; (c) coating an epoxy resin onto the titanium substrate on which the concave shape has been formed; and (d) obtaining an epoxy substrate having a nanopattern of convex surfaces by removing the titanium substrate. According to the presently disclosed method, an epoxy substrate having a nanopattern of convex surfaces is prepared by anodizing a titanium substrate, coating an epoxy resin onto a nanopattern formed with a concave shape on the surface of the titanium substrate, and removing the titanium substrate. This straightforward process makes it possible to efficiently prepare an epoxy substrate having a nanopattern, which is fine and on the nano scale, for the measurement of blood coagulation. Further, the epoxy substrate prepared in accordance with the present disclosure makes it possible to efficiently use an electrochemical technique to ascertain whether or not blood coagulation is present in a blood sample.
摘要翻译： 提供一种制备具有纳米图案的环氧树脂基板的方法，包括：（a）通过阳极氧化钛基板形成氧化钛膜; （b）从形成有氧化钛膜的钛基板除去氧化钛膜，得到表面形成有凹状的钛基板; （c）将环氧树脂涂布到已经形成凹形的钛基板上; 和（d）通过除去钛基材得到具有凸表面的纳米图案的环氧树脂基材。 根据目前公开的方法，通过对钛基板进行阳极氧化，在钛基板的表面上将环氧树脂涂布在形成为凹形的纳米图案上，并除去钛基板，来制备具有凸面的纳米图案的环氧树脂基板。 这种简单的方法使得可以有效地制备具有细小且纳米尺度的纳米图案用于测量血液凝固的环氧树脂基材。 此外，根据本公开制备的环氧树脂基材使得可以有效地使用电化学技术来确定血液样品中是否存在凝血。
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3.发明申请Mutated Nucleotide Sequences of Batroxobin, Mutated Alpha Factor Secretion Signal Sequence and Processes for Preparing Batroxobin Using the Same 有权巴曲酶的突变核苷酸序列，突变的α因子分泌信号序列和使用其制备巴曲酶的过程公开(公告)号：US20110136207A1
公开(公告)日：2011-06-09
申请号：US12810902
申请日：2008-12-23
申请人： Young-Doug Sohn , Bum Joon Kim , Ok Hwan Kim , Kyoung Jun Kim , Ji Hun Shin , Sung Yu Hong , Jae Hoon Hwang , Kwang Hoe Chung
发明人： Young-Doug Sohn , Bum Joon Kim , Ok Hwan Kim , Kyoung Jun Kim , Ji Hun Shin , Sung Yu Hong , Jae Hoon Hwang , Kwang Hoe Chung
CPC分类号： C12N9/6418 , C07K2319/02
摘要： The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.
摘要翻译： 本发明涉及一种编码巴曲酶的核苷酸序列和/或突变的α因子分泌信号序列，以及使用其的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高出4-13倍的产量获得重组巴曲酶。 使用巴曲酶编码核苷酸序列以及本发明的突变型α因子分泌信号肽序列的蛋白质表达系统以比天然存在的巴曲酶编码序列高出约20倍的产量获得重组巴曲酶。 此外，使用本发明的序列制备的重组巴曲酶与天然存在的巴曲酶相比具有显着似乎合理的活性和稳定性。
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4.发明申请公开(公告)号：US20100120849A1
公开(公告)日：2010-05-13
申请号：US12528224
申请日：2007-05-23
申请人： Kwang Hoe Chung , Chwang Siek Pak , Sung Yu Hong , Soo Jung Kang , Young Doug Sohn , Jae Hoon Hwang , Eun Bok Choi , Gyu Hwan Yon , Hyeon Kyu Lee , Heui Cheol Yang
发明人： Kwang Hoe Chung , Chwang Siek Pak , Sung Yu Hong , Soo Jung Kang , Young Doug Sohn , Jae Hoon Hwang , Eun Bok Choi , Gyu Hwan Yon , Hyeon Kyu Lee , Heui Cheol Yang
CPC分类号： A61K31/47
摘要： The present invention relates to a pharmaceutical composition for preventing or treating hyperproliferative vascular disorders, and a pharmaceutical anticancer composition comprising the compound represented by the Formula 1. The present compounds exhibit IC50 values of less than 0.16 μM for vascular smooth muscle cells and cancer cells to effectively prevent proliferation of vascular smooth muscle cells and cancer cells, thereby ensuring prevention or treatment of hyperproliferative vascular disorders such as arteriosclerosis and restenosis, and cancers.
摘要翻译： 本发明涉及一种用于预防或治疗过度增殖性血管疾病的药物组合物，以及包含由式1表示的化合物的药物抗癌组合物。本发明化合物显示血管平滑肌细胞和癌细胞的IC 50值小于0.16μM 有效防止血管平滑肌细胞和癌细胞的增殖，从而确保预防或治疗过度增生性血管疾病如动脉硬化和再狭窄以及癌症。
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5.发明授权Mutated nucleotide sequences of batroxobin, mutated α factor secretion signal sequence and processes for preparing batroxobin using the same 有权巴曲酶的突变核苷酸序列，突变的α因子分泌信号序列和使用其制备巴曲酶的方法公开(公告)号：US08377676B2
公开(公告)日：2013-02-19
申请号：US12810902
申请日：2008-12-23
申请人： Young-Doug Sohn , Bum Joon Kim , Ok Hwan Kim , Kyoung Jun Kim , Ji Hun Shin , Sung Yu Hong , Jae Hoon Hwang , Kwang Hoe Chung
发明人： Young-Doug Sohn , Bum Joon Kim , Ok Hwan Kim , Kyoung Jun Kim , Ji Hun Shin , Sung Yu Hong , Jae Hoon Hwang , Kwang Hoe Chung
CPC分类号： C12N9/6418 , C07K2319/02
摘要： The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.
摘要翻译： 本发明涉及一种编码巴曲酶的核苷酸序列和/或突变的α因子分泌信号序列，以及使用其的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高出4-13倍的产量获得重组巴曲酶。 使用巴曲酶编码核苷酸序列以及本发明的突变型α因子分泌信号肽序列的蛋白质表达系统以比天然存在的巴曲酶编码序列高出约20倍的产量获得重组巴曲酶。 此外，使用本发明的序列制备的重组巴曲酶与天然存在的巴曲酶相比具有显着似乎合理的活性和稳定性。
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6.发明授权公开(公告)号：US08106071B2
公开(公告)日：2012-01-31
申请号：US12528224
申请日：2007-05-23
申请人： Kwang Hoe Chung , Chwang Siek Pak , Sung Yu Hong , Soo Jung Kang , Young Doug Sohn , Jae Hoon Hwang , Eun Bok Choi , Gyu Hwan Yon , Hyeon Kyu Lee , Heui Cheol Yang
发明人： Kwang Hoe Chung , Chwang Siek Pak , Sung Yu Hong , Soo Jung Kang , Young Doug Sohn , Jae Hoon Hwang , Eun Bok Choi , Gyu Hwan Yon , Hyeon Kyu Lee , Heui Cheol Yang
IPC分类号： A01N43/42
CPC分类号： A61K31/47
摘要： The present invention relates to a method for treating or reducing the development of a hyperproliferative disorder, which comprises administering to a subject a composition, which comprises as an active ingredient the compound represented by the Formula 1: wherein R1, R2, R3, R4, R5, R6 and R7 independently represent hydrogen, halo, hydroxyl, cyano, amino, nitro, nitroso, carboxyl, C1-C12 alkyl, C2-C6 alkenyl, C3-C8 cycloalkyl, C5-C7 cycloalkenyl, C1-C6 alkylamino, C1-C6 alkoxy, aryl, heteroaryl, arylalkyl, arylalkenyl or alkylaryl; X and Y independently represent hydrogen, oxygen, or sulfur, bound to a carbon atom via a single or double bond; Z represents hydrogen, halo, hydroxyl, cyano, amino, nitro, nitroso, carboxyl, C1-C12 alkyl, C2-C6 alkenyl, C3-C8 cycloalkyl, C5-C7 cycloalkenyl, C1-C6 alkylamino, C1-C6 alkoxy, aryl, heteroaryl, arylalkyl, arylalkenyl, alkylaryl or —NH—R8; R8 represents hydrogen, halo, hydroxyl, cyano, amino, nitro, nitroso, carboxyl, C1-C12 alkyl, C2-C6 alkenyl, C3-C8 cycloalkyl, C5-C7 cycloalkenyl, C1-C6 alkylamino, C1-C6 alkoxy, aryl, heteroaryl, arylalkyl, arylalkenyl or alkylaryl; n is an integer of 0-3.
摘要翻译： 本发明涉及一种治疗或减轻过度增殖性疾病发展的方法，其包括向受试者施用组合物，其包含作为活性成分的由式1表示的化合物：其中R1，R2，R3，R4， R5，R6和R7独立地表示氢，卤素，羟基，氰基，氨基，硝基，亚硝基，羧基，C1-C12烷基，C2-C6烯基，C3-C8环烷基，C5-C7环烯基，C1-C6烷基氨基，C1- C6烷氧基，芳基，杂芳基，芳基烷基，芳基烯基或烷基芳基; X和Y独立地表示通过单键或双键与碳原子结合的氢，氧或硫; Z代表氢，卤素，羟基，氰基，氨基，硝基，亚硝基，羧基，C1-C12烷基，C2-C6烯基，C3-C8环烷基，C5-C7环烯基，C1-C6烷基氨基，C1-C6烷氧基， 杂芳基，芳基烷基，芳基烯基，烷基芳基或-NH-R8; R8代表氢，卤素，羟基，氰基，氨基，硝基，亚硝基，羧基，C1-C12烷基，C2-C6烯基，C3-C8环烷基，C5-C7环烯基，C1-C6烷基氨基，C1-C6烷氧基， 杂芳基，芳基烷基，芳基烯基或烷基芳基; n为0-3的整数。
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公开(公告)号：US09982021B2
公开(公告)日：2018-05-29
申请号：US13882792
申请日：2011-11-01
申请人： Ji Hoe Heo , Il Kwon , Young Dae Kim , Sung Yu Hong
发明人： Ji Hoe Heo , Il Kwon , Young Dae Kim , Sung Yu Hong
CPC分类号： C07K14/001 , A61K38/06 , A61K38/1703 , C07K7/06 , C07K14/46 , C12N9/6489
摘要： The present invention relates to: a composition for dissolving thrombi comprising a peptide comprising the Arg-Gly-Asp motif; a pharmaceutical composition for treating stenotic or occlusive vascular diseases which comprises the same; and a thrombus dissolving method and a method for treating stenotic or occlusive vascular diseases, comprising the step of administering the same. The compositions and methods of the present invention have the advantage that they effectively break down already formed thrombi by adopting the principle of targeting integrin within the thrombus such as platelet surface GPIIb-IIIa, which is not the same as the existing principle of plasminogen activation. Also, the compositions and methods of the present invention have a nerve-protecting function as they effectively open as far as the microvasculature, without the occurrence of restenosis after penetration.
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8.发明申请COMPOSITION FOR DISSOLVING THROMBI, AND A PHARMACEUTICAL COMPOSITION FOR TREATING STENOTIC OR OCCLUSIVE VASCULAR DISEASES WHICH COMPRISES THE SAME 有权用于溶解THROMBI的组合物和用于治疗包含其的STEOTIC或OCCLUSIVE血管疾病的药物组合物公开(公告)号：US20130316951A1
公开(公告)日：2013-11-28
申请号：US13882792
申请日：2011-11-01
申请人： Ji Hoe Heo , Il Kwon , Young Dae Kim , Sung Yu Hong
发明人： Ji Hoe Heo , Il Kwon , Young Dae Kim , Sung Yu Hong
CPC分类号： C07K14/001 , A61K38/06 , A61K38/1703 , C07K7/06 , C07K14/46 , C12N9/6489
摘要： The present invention relates to: a composition for dissolving thrombi comprising a peptide comprising the Arg-Gly-Asp motif; a pharmaceutical composition for treating stenotic or occlusive vascular diseases which comprises the same; and a thrombus dissolving method and a method for treating stenotic or occlusive vascular diseases, comprising the step of administering the same. The compositions and methods of the present invention have the advantage that they effectively break down already formed thrombi by adopting the principle of targeting integrin within the thrombus such as platelet surface GPIIb-IIIa, which is not the same as the existing principle of plasminogen activation. Also, the compositions and methods of the present invention have a nerve-protecting function as they effectively open as far as the microvasculature, without the occurrence of restenosis after penetration.
摘要翻译： 本发明涉及：用于溶解血栓的组合物，其包含含有Arg-Gly-Asp基序的肽; 用于治疗狭窄或闭塞性血管疾病的药物组合物，其包含其; 和血栓溶解方法以及治疗狭窄或闭塞性血管疾病的方法，包括施用该血管疾病的步骤。 本发明的组合物和方法的优点在于，通过采用靶向血栓内的整联蛋白如血小板表面GPIIb-IIIa的原理，有效地分解了已经形成的血栓，这与现有的纤溶酶原激活原理不同。 此外，本发明的组合物和方法具有神经保护功能，因为它们有效地开放至微血管，而不会在穿透后再发生再狭窄。
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