公开(公告)号：US20060018923A1

公开(公告)日：2006-01-26

申请号：US10895064

申请日：2004-07-21

申请人： Kwok Yuen ,  Chiu Woo ,  Kar Lau ,  Kwok Chan ,  Lit Poon ,  Joseph Peiris ,  Yi Guan

发明人： Kwok Yuen ,  Chiu Woo ,  Kar Lau ,  Kwok Chan ,  Lit Poon ,  Joseph Peiris ,  Yi Guan

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N15/86 ,  C07K14/01

CPC分类号： G01N33/56983 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20022

摘要： The present invention provides the complete genomic sequence of a novel human coronavirus, coined as coronavirus-HKU1 (“CoV-HKU1”), isolated in Hong Kong from a patient who had a recent history of visit to Schenzhen, China. The virus belongs to the order Nidovirales of the family Coronaviridae, being a single-stranded RNA virus of positive polarity. The invention also provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the CoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by CoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the CoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the CoV-HKU1.

摘要翻译： 本发明提供了一种新型人冠状病毒的完整基因组序列，其创造为冠状病毒HKU1（“CoV-HKU1”），在香港从最近访问中国的深圳的病人中分离出来。 该病毒属于冠状病毒科家族的Nidovirales，是正极性的单链RNA病毒。 本发明还提供了CoV-HKU1的完整基因组的推导的氨基酸序列。 CoV-HKU1的核苷酸序列和推导的氨基酸序列可用于预防，诊断和/或治疗CoV-HKU1感染。 此外，本发明基于CoV-HKU1的核苷酸序列和推定的氨基酸序列，提供使用重组和嵌合形式以及CoV-HKU1的亚基的免疫原性和疫苗制剂。

公开(公告)号：US20050181357A1

公开(公告)日：2005-08-18

申请号：US10807807

申请日：2004-03-24

申请人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls ,  Frederick Leung

发明人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls ,  Frederick Leung

IPC分类号： A61P11/00 ,  A61P31/14 ,  C12N15/50 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/02 ,  C07K14/165 ,  C07K16/10

CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  Y02A50/451

摘要： The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated coronavirus (SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.

摘要翻译： 本发明涉及用于引起人类严重急性呼吸综合征（SARS）的病毒（“hSARS病毒”）的高通量诊断测定法。 特别地，本发明涉及SARS相关冠状病毒（SARS-CoV）的高通量逆转录PCR诊断试验。 本试验是一种快速，可靠的测定法，可用于诊断和监测SARS的扩散，并且基于hSARS病毒的N（核衣壳）基因的核苷酸序列。 本方法消除假阴性结果并提供增加的检测灵敏度。 本发明还公开了hSARS病毒的S（尖峰）基因。 本发明还涉及hSARS病毒的N-基因和S-基因产物的推断氨基酸序列以及在诊断方法中使用N-基因和S-基因产物。 本发明还包括诊断测定和试剂盒，其包含针对N-基因或S-基因产物产生的抗体。

公开(公告)号：US20050009009A1

公开(公告)日：2005-01-13

申请号：US10808187

申请日：2004-03-24

申请人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls

发明人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls

IPC分类号： C07H21/04 ,  C07K16/08 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/701 ,  C07H21/04 ,  C12Q1/6844

摘要： The present invention relates to a diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a real-time quantitative PCR assay for the detection of hSARS virus using reverse transcription and polymerase chain reaction. Specifically, the quantitative assay is a TaqMan® assay using the primers and probes constructed based on the genome of the hSARS virus. The invention further relates to a diagnostic kit that comprises nucleic acid molecules for the detection of the hSARS virus.

摘要翻译： 本发明涉及在人类中引起严重急性呼吸综合征（SARS）的病毒的诊断测定法（“hSARS病毒”）。 特别地，本发明涉及使用逆转录和聚合酶链反应检测hSARS病毒的实时定量PCR测定。 具体地，定量测定是使用基于hSARS病毒基因组构建的引物和探针的TaqMan测定法。 本发明还涉及包含用于检测hSARS病毒的核酸分子的诊断试剂盒。

公开(公告)号：US20060282921A1

公开(公告)日：2006-12-14

申请号：US11402181

申请日：2006-04-10

申请人： Eric Lam ,  Lit Poon ,  Yuanxiang Zhou ,  Mee Chye ,  Joseph Peiris

发明人： Eric Lam ,  Lit Poon ,  Yuanxiang Zhou ,  Mee Chye ,  Joseph Peiris

IPC分类号： A01H1/00 ,  C07H21/02 ,  A61K48/00 ,  C12N5/04 ,  C12N5/06 ,  C12N15/82

CPC分类号： C12N15/8258 ,  A61K9/0019 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/8218 ,  C12N15/8257 ,  C12N2310/14 ,  C12N2330/30 ,  C12N2760/16111

摘要： The present invention provides plant-derived agents to interfere with the nonstructural NS1 gene from the influenza A virus subtype H1N1. More particularly, the siRNAs that exhibit strong inhibitory activity towards NS1, which effectively suppress replication of the influenza virus in mammalian cells. The invention further provides methods for production of siRNAs for the suppression of a broad range of influenza viral subtypes with sequence homologies.

摘要翻译： 本发明提供干扰来自甲型流感病毒亚型H1N1的非结构NS1基因的植物衍生试剂。 更具体地，对NS1表现出强抑制活性的siRNA，其有效地抑制了哺乳动物细胞中流感病毒的复制。 本发明还提供了用于抑制具有序列同源性的广泛流感病毒亚型的siRNA的制备方法。

公开(公告)号：US20060053516A1

公开(公告)日：2006-03-09

申请号：US11004399

申请日：2004-12-03

申请人： Mee Chye ,  Hong Li ,  Sathiskumar Ramalingam ,  Leo Poon ,  Joseph Peiris

发明人： Mee Chye ,  Hong Li ,  Sathiskumar Ramalingam ,  Leo Poon ,  Joseph Peiris

IPC分类号： A61K39/12 ,  C12N5/04 ,  A01H1/00 ,  C12N15/82 ,  A61K36/18

CPC分类号： C12N15/8257 ,  A61K31/7088 ,  A61K39/12 ,  A61K39/215 ,  A61K2039/517 ,  A61K2039/542 ,  C12N2770/20034

摘要： The invention relates to genetically modified plants and progeny thereof which constitute edible plant-derived mucosal vaccines and injectable plant-derived mucosal vaccines against Severe Acute Respiratory Syndrome (SARS). The invention relates to a recombinant vector that transforms specifically, but not limited to, the nuclei and/or plastids of tobacco, tomato and lettuce plants for antigen production. In specific embodiments, the plastid transformation vector expressing the nucleotide sequences are pCV1, pCV6 and pCV8, and their derivatives containing an S1 with nucleotide (but not amino acid) changes to optimize codon usage for expression in plants. The present invention relates to methods of making the modified plants which comprises transformation of plants with vectors for nuclear expression and/or plastid expression of nucleotide sequences of the SARS-CoV virus, fragments, derivatives, analogs, or variants thereof. The present invention also relates to methods of immunization against SARS and methods of antibody detection using the SARS-CoV antigens generated by the plastid and/or nuclear vector(s) transformed plants.

摘要翻译： 本发明涉及构成食用植物来源的粘膜疫苗和针对严重急性呼吸综合征（SARS）的可注射植物来源的粘膜疫苗的转基因植物及其后代。 本发明涉及一种特异性转化，但不限于烟草，番茄和莴苣植物的核和/或质体用于抗原生产的重组载体。 在具体实施方案中，表达核苷酸序列的质体转化载体是pCV1，pCV6和pCV8，并且其含有具有核苷酸（但不是氨基酸）的S1的衍生物改变以优化用于在植物中表达的密码子使用。 本发明涉及制备修饰植物的方法，其包括用SARS-CoV病毒，其片段，衍生物，类似物或变体的核表达和/或质体表达的载体转化植物。 本发明还涉及使用由质体和/或核载体转化的植物产生的SARS-CoV抗原的SARS免疫方法和抗体检测方法。