-
公开(公告)号：US20090054258A1
公开(公告)日：2009-02-26
申请号：US12256256
申请日：2008-10-22
申请人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
发明人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.
摘要翻译： 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。 在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。 在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。
-
公开(公告)号：US07824863B2
公开(公告)日：2010-11-02
申请号：US12256256
申请日：2008-10-22
申请人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
发明人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.
摘要翻译： 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。 在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。 在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。
-
公开(公告)号：US07504215B2
公开(公告)日：2009-03-17
申请号：US10983046
申请日：2004-11-04
申请人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
发明人： Kyle B. Cole , Vivi Truong , Glenn H. McGall , Anthony D. Barone
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule.In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: providing the sample comprising RNA which may or may not have said RNA of interest; treating the sample with a fragmenting reagent to provide RNA fragments; removing phosphate groups from said fragments to provide fragments with free 3′ OH groups; ligating said fragment with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to said RNA of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of said RNA of interest.
摘要翻译： 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在本发明的一个方面，T4 RNA连接酶用于将3'标记的AMP或CMP供体连接到RNA受体分子上。 在另一个实施方案中，将焦磷酸盐分子3'- AppN-3'-接头可检测部分用作供体分子。 在本发明的另一方面，提供了检测样品中目的RNA存在的方法，该方法具有以下步骤：提供包含可能具有或不具有所述目的RNA的RNA的样品; 用碎裂试剂处理样品以提供RNA片段; 从所述片段中除去磷酸基以提供具有游离的3'OH基团的片段; 将所述片段与根据本发明的标记试剂连接; 提供具有针对所述感兴趣的RNA的探针的核酸阵列; 将标记的核酸片段与所述核酸阵列杂交; 以及确定与所述探针的杂交程度，以确定所述目的RNA的存在。
-
4.发明申请Methods for Analyzing Global Regulation of Coding and Non-Coding RNA Transcripts Involving Low Molecular Weight RNAs 审中-公开分析涉及低分子量RNA的编码和非编码RNA转录本的全局调控方法公开(公告)号：US20080261817A1
公开(公告)日：2008-10-23
申请号：US11554895
申请日：2006-10-31
申请人： Philipp Kapranov , Dione Kampa , Thomas R. Gingeras , Stefan Bekiranov , Simon Cawley , Kyle B. Cole
发明人： Philipp Kapranov , Dione Kampa , Thomas R. Gingeras , Stefan Bekiranov , Simon Cawley , Kyle B. Cole
IPC分类号： C40B30/00
CPC分类号： C12Q1/6837 , C12Q1/6876 , C12Q2600/158 , C12Q2600/178 , C12Q2565/507
摘要： In some embodiments of the invention, methods are provided to interrogate the transcriptional activity relating to RNAs of small molecular weight. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs of small molecular weight.
摘要翻译： 在本发明的一些实施方案中，提供了用于询问与小分子量的RNA相关的转录活性的方法。 该方法使用大量寡核苷酸探针与衍生自小分子量RNA的核酸杂交。
-
-
-
搜索结果
4 条记录 (耗时 0.02 秒)
