公开(公告)号：US5015588A

公开(公告)日：1991-05-14

申请号：US114630

申请日：1987-10-29

申请人： Kyung Lee ,  Manford K. Patterson, Jr. ,  Paul J. Birckbichler

发明人： Kyung Lee ,  Manford K. Patterson, Jr. ,  Paul J. Birckbichler

IPC分类号： C12Q1/56 ,  G01N33/86

CPC分类号： G01N33/86 ,  C12Q1/56 ,  G01N2333/9108 ,  G01N2333/91085 ,  Y10T436/25125 ,  Y10T436/255

摘要： An assay method for the detection of Factor XIII in plasma is disclosed in which a primary amine derivative of biotin such as preferably 5-(biotinamido) pentylamine is incubated with a glutamine substrate and activated Factor XIII (Factor XIIIa) to form a biotinylated product which may be measured by convention detection assays. In a preferred embodiment, the biotinylated product is bound to a well in a microtiter plate or other solid support and the product is measured by a colorimetric assay which may be read by an automated spectrophotometric plate reader.

摘要翻译： 公开了一种用于检测血浆中因子XIII的测定方法，其中生物素的伯胺衍生物如优选5-（生物素酰氨基）戊胺与谷氨酰胺底物和活化因子XIII（因子XIIIa）一起孵育以形成生物素化产物， 可以通过常规检测测定来测量。 在优选的实施方案中，生物素化的产物在微量滴定板或其它固体支持物中与孔结合，并且通过比色测定法测量产物，其可以通过自动分光光度计读数器读取。

公开(公告)号：US5726051A

公开(公告)日：1998-03-10

申请号：US247902

申请日：1994-05-23

申请人： Bassam M. Fraij ,  Paul J. Birckbichler ,  Manford K. Patterson, Jr. ,  Robert A. Gonzales

发明人： Bassam M. Fraij ,  Paul J. Birckbichler ,  Manford K. Patterson, Jr. ,  Robert A. Gonzales

IPC分类号： C12N9/10 ,  C12N15/54

CPC分类号： C12N9/1044

摘要： The present invention relates to a 1.9 kb cDNA and new tissue transglutaminase protein encoded thereby. The cDNA was obtained from reverse transcription of mRNA isolated from retinoic-acid treated HEL cells. The invention also relates to vectors and expression systems to produce the new tissue transglutaminase as well as the recombinantly-produced enzyme protein.

摘要翻译： 本发明涉及由其编码的1.9kb cDNA和新的组织转谷氨酰胺酶蛋白。 cDNA从从视黄酸处理的HEL细胞中分离的mRNA的逆转录获得。 本发明还涉及产生新的组织转谷氨酰胺酶以及重组产生的酶蛋白质的载体和表达系统。